##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064123_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	797032
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.288320920615483	32.0	32.0	32.0	32.0	32.0
2	30.94991167230425	32.0	32.0	32.0	32.0	32.0
3	30.981231619307632	32.0	32.0	32.0	32.0	32.0
4	30.98548113501089	32.0	32.0	32.0	32.0	32.0
5	30.990108301799676	32.0	32.0	32.0	32.0	32.0
6	34.635450019572616	36.0	36.0	36.0	32.0	36.0
7	34.567337321462624	36.0	36.0	36.0	32.0	36.0
8	34.52467027672665	36.0	36.0	36.0	32.0	36.0
9	34.587612542532796	36.0	36.0	36.0	32.0	36.0
10	34.365922572744886	36.0	36.0	36.0	32.0	36.0
11	34.57943595740196	36.0	36.0	36.0	32.0	36.0
12	34.436988728181554	36.0	36.0	36.0	32.0	36.0
13	34.48258790111313	36.0	36.0	36.0	32.0	36.0
14	34.43343805518474	36.0	36.0	36.0	32.0	36.0
15	34.415576789890494	36.0	36.0	36.0	32.0	36.0
16	34.39133435044013	36.0	36.0	36.0	32.0	36.0
17	34.36474445191661	36.0	36.0	36.0	32.0	36.0
18	34.364291521544935	36.0	36.0	36.0	32.0	36.0
19	34.28829331821056	36.0	36.0	36.0	32.0	36.0
20	34.24987704383262	36.0	36.0	36.0	32.0	36.0
21	34.2177440805388	36.0	36.0	36.0	32.0	36.0
22	34.1714862138534	36.0	36.0	36.0	32.0	36.0
23	34.17450491322808	36.0	36.0	36.0	32.0	36.0
24	34.13276380371177	36.0	36.0	36.0	32.0	36.0
25	33.78809257344749	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	8.0
5	13.0
6	24.0
7	11.0
8	49.0
9	47.0
10	77.0
11	28.0
12	50.0
13	37.0
14	70.0
15	142.0
16	242.0
17	287.0
18	411.0
19	554.0
20	753.0
21	1065.0
22	1713.0
23	2533.0
24	3751.0
25	5531.0
26	7833.0
27	10510.0
28	14244.0
29	19322.0
30	25358.0
31	34747.0
32	49173.0
33	71325.0
34	162736.0
35	384384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46179529179925	19.773690057048178	12.035722578904098	25.728792072248474
2	11.795646522086097	20.784042601245716	47.73053507525986	19.68977580140833
3	18.864693143693113	26.79023560321097	30.131439942385242	24.213631310710674
4	10.439120604465234	17.617772602829103	40.67492929754254	31.268177495163123
5	10.25003670182932	41.12788172098328	37.31026409005186	11.311817487135539
6	29.036075184554516	40.13042730817059	19.39607413049507	11.437423376779824
7	26.069774738030997	32.214971450084704	23.267616238940832	18.447637572943464
8	22.12004412888748	43.51201181790684	20.308826907074554	14.059117146131129
9	27.236540836923446	14.456737866417619	20.69198827061931	37.61473302603962
10	14.768179330223308	29.68612691510036	36.47039032645211	19.07530342822422
11	33.98403173566999	20.305305179642975	28.965075698611564	16.74558738607547
12	25.53639447497997	23.656722601510065	34.901609748381084	15.905273175128873
13	30.91475204017577	23.217451349654738	26.182925298179537	19.68487131198996
14	18.432831417196393	25.52741371106153	28.02992161771593	28.009833254026145
15	19.73284958351903	39.27311987245074	22.598188449008543	18.395842095021685
16	18.124065441387874	27.616607177159043	34.79602610926065	19.46330127219243
17	18.293610738659474	31.317198226293247	31.1285033470429	19.26068768800438
18	18.377720018583393	27.276653984756592	37.30616139927926	17.039464597380746
19	23.926347245241324	25.34026417558134	27.88722314298629	22.84616543619105
20	23.794240481374608	29.057391767308815	30.015846586956574	17.132521164359996
21	21.457289082197423	26.425245848944783	26.990397074924914	25.12706799393288
22	22.863557485365284	32.56064209423978	27.272556046379055	17.30324437401588
23	19.110690871465685	31.93662250383301	30.201501561458166	18.751185063243135
24	23.637167241537345	27.451152733952693	31.027823573506648	17.883856451003314
25	20.84684705046251	30.106364411429748	31.17201753361635	17.87477100449139
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	118.0
2	269.0
3	420.0
4	420.0
5	420.0
6	1309.0
7	2198.0
8	2198.0
9	2198.0
10	2222.0
11	2246.0
12	2246.0
13	2246.0
14	2661.0
15	3076.0
16	3076.0
17	3076.0
18	5744.5
19	8413.0
20	8413.0
21	8413.0
22	15714.0
23	23015.0
24	23015.0
25	23015.0
26	38808.5
27	54602.0
28	54602.0
29	54602.0
30	76296.5
31	97991.0
32	97991.0
33	97991.0
34	101998.5
35	106006.0
36	106006.0
37	106006.0
38	105588.0
39	105170.0
40	105170.0
41	105170.0
42	104685.5
43	104201.0
44	104201.0
45	104201.0
46	104570.0
47	104939.0
48	104939.0
49	104939.0
50	92323.5
51	79708.0
52	79708.0
53	79708.0
54	62192.0
55	44676.0
56	44676.0
57	44676.0
58	35947.0
59	27218.0
60	27218.0
61	27218.0
62	21452.0
63	15686.0
64	15686.0
65	15686.0
66	12308.5
67	8931.0
68	8931.0
69	8931.0
70	6522.5
71	4114.0
72	4114.0
73	4114.0
74	3201.5
75	2289.0
76	2289.0
77	2289.0
78	1611.0
79	933.0
80	933.0
81	933.0
82	680.0
83	427.0
84	427.0
85	427.0
86	307.0
87	187.0
88	187.0
89	187.0
90	137.0
91	87.0
92	87.0
93	87.0
94	68.5
95	50.0
96	50.0
97	50.0
98	190.5
99	331.0
100	331.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04228186572182798
2	0.0075279286151622524
3	0.001756516676871192
4	0.0037639643075811262
5	0.00865711790743659
6	0.014177598891888906
7	0.022834716799325496
8	0.03450300615282698
9	0.05018619076774834
10	0.05871784319826556
11	0.056710395567555624
12	0.06398739322887914
13	0.0667476337211053
14	0.06913147778257335
15	0.05984703249053991
16	0.06687309919802467
17	0.0637364622750404
18	0.07803952664384868
19	0.07427556233626754
20	0.08105069808991358
21	0.07628300996697748
22	0.0800469742745586
23	0.08293268024370415
24	0.07791406116692931
25	0.07766313021309057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	797032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.664850655623304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.31679123427294	40.185733823676905
2	12.859488150712888	13.03048093327252
3	3.5857948327022076	5.450212790416894
4	1.3934148771519699	2.82388626608913
5	0.6688406183999465	1.6943355021824011
6	0.39818026637102094	1.2104246237822451
7	0.2743360635664021	0.9729436983030344
8	0.1985329656035294	0.8046914442016653
9	0.14972635053172076	0.6827276870011024
>10	0.9547169744946268	9.425710896080645
>50	0.0995922417878398	3.5814019121474425
>100	0.0832002893229425	8.094063201146223
>500	0.009189285686217461	3.2724803990632836
>1k	0.007202413105413686	5.835275349950426
>5k	9.934362904018878E-4	2.9356314726860617
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	6868	0.861696895482239	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5649	0.708754479117526	No Hit
GTCCTACAGTGGACATTTCTAAATT	5440	0.6825321944413775	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5373	0.6741260074877796	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3603	0.4520521133404932	No Hit
ATTTAGAAATGTCCACTGTAGGACG	3000	0.3763964307581126	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2722	0.3415170281745275	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2692	0.33775306386694637	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2363	0.29647492196047337	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2147	0.2693743789458892	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1999	0.25080548836182237	No Hit
GATATACACTGTTCTACAAATCCCG	1721	0.21592608577823724	No Hit
GAATATGGCAAGAAAACTGAAAATC	1714	0.2150478274398017	No Hit
TTGTAGAACAGTGTATATCAATGAG	1617	0.20287767617862268	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1566	0.19647893685573475	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1552	0.1947224201788636	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1489	0.18681809513294323	No Hit
GTGTATATCAATGAGTTACAATGAA	1272	0.15959208664143976	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1267	0.1589647592568429	No Hit
GCCATATTCCACGTCCTACAGTGGA	1265	0.15871382830300415	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1238	0.15532626042618114	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1199	0.15043310682632566	No Hit
CTGTAGGACATGGAATATGGCAAGA	1183	0.14842565919561573	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1130	0.14177598891888907	No Hit
GTGTATATCAATGAGTTACAATGAG	1119	0.140395868672776	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1104	0.13851388651898544	No Hit
GTCCACTGTAGGACGTGGAATATGG	1097	0.13763562818054983	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1091	0.1368828353190336	No Hit
GACCTGGAATATGGCGAGAAAACTG	1076	0.13500085316524307	No Hit
ATACACACTTTAGGACGTGAAATAT	1066	0.13374619839604934	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1052	0.13198968171917816	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1021	0.12810025193467767	No Hit
CCACTGTAGGACGTGGAATATGGCA	1009	0.1265946662116452	No Hit
GATATACACTGTTCTACAATGCCGG	996	0.12496361501169338	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	943	0.11831394473496672	No Hit
GTACATGGGGTGGTATCAACGCAAA	942	0.11818847925804735	No Hit
GTGTATTTCTCATTTTCCGTGATTT	942	0.11818847925804735	No Hit
CACTTTAGGACGTGAAATATGGCGA	934	0.11718475544269238	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	911	0.11429904947354685	No Hit
GAAATACACACTTTAGGACGTGAAA	909	0.11404811851970813	No Hit
CTGTAGAACATATTAGATGAGTGAG	872	0.1094058958736914	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	840	0.10539100061227154	No Hit
CTACAGTGGACATTTCTAAATTTTC	836	0.10488913870459404	No Hit
GTAGGACGTGGAATATGGCAAGAAA	802	0.10062331248933544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2546547691937086E-4	0.0
3	0.0	0.0	0.0	1.2546547691937086E-4	0.0
4	0.0	0.0	0.0	1.2546547691937086E-4	0.0
5	0.0	0.0	0.0	1.2546547691937086E-4	0.0
6	0.0	0.0	0.0	1.2546547691937086E-4	0.0
7	0.0	0.0	0.0	1.2546547691937086E-4	0.0
8	0.0	0.0	0.0	1.2546547691937086E-4	0.0
9	0.0	0.0	0.0	1.2546547691937086E-4	0.0
10	0.0	0.0	0.0	1.2546547691937086E-4	0.0
11	0.0	0.0	0.0	1.2546547691937086E-4	0.0
12	0.0	0.0	0.0	1.2546547691937086E-4	0.0
13	0.0	0.0	0.0	1.2546547691937086E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTT	25	0.0060269074	19.001444	12
GTTCCGA	30	7.717528E-4	19.000252	6
AGCTCGT	25	0.006029093	19.000252	11
TCCGACC	35	0.0021669418	16.287973	8
CGACCAT	35	0.0021669418	16.287973	10
CCGACCA	35	0.0021669418	16.287973	9
TTCCGAC	35	0.0021678507	16.286951	7
AGTTCCG	45	6.75975E-4	14.777046	5
GTTCTAA	65	3.373265E-6	14.612825	1
GCAATTT	40	0.005273456	14.251082	13
AATCGTA	40	0.005273456	14.251082	13
CCAACGT	75	9.64741E-7	13.934392	19
ATAATAC	55	1.9643106E-4	13.813162	3
TAGGACC	955	0.0	13.727069	4
CACCTTT	670	0.0	12.761363	14
GGCGAGG	1385	0.0	12.759092	19
CCACCTT	680	0.0	12.714202	13
TTCCACC	710	0.0	12.711436	11
TTAAGGT	60	4.089846E-4	12.6660385	4
TTGTAGA	385	0.0	12.335502	1
>>END_MODULE