FastQCFastQC Report
Thu 2 Feb 2017
SRR4064123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797032
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA29560.3708759497736603No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA25430.31905870780596013No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA22710.28493209808389125No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC19930.2500526955003061No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA15450.19384416184042796No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA15280.19171124873279868No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT9910.12433628762709653No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG9610.12057232331951541No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA9170.11505184233506309No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA8550.10727298276606209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATA250.00235302135.1978411
TCACGCT407.0273795E-427.50176427
TAGGACC11250.026.598934
CTAGGAC2200.024.001543
AGGACCT22950.022.14665
GGACCTG21850.021.9524446
GTCCTAT2050.021.4620971
TGTAGGA36700.020.7398742
CGCGGTA856.1123683E-620.70461343
TACGGAA755.472219E-520.53207637
GATATAC8700.020.4815041
ATAGGAC2150.020.466433
TATAGGA2300.020.0857262
CTGTAGG35450.020.0439051
AGGCGTG550.00447900420.0025397
AGAGTGT2750.020.0025396
CAGTCCG550.00447900420.0025399
ATTACGT550.00448388619.99877242
CTCTAGG2100.019.9035421
TTAGGAC23650.019.8152243
Produced by FastQC (version 0.11.5)