##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	797032
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33405936022644	32.0	32.0	32.0	32.0	32.0
2	31.472996065402643	32.0	32.0	32.0	32.0	32.0
3	31.533727629505464	32.0	32.0	32.0	32.0	32.0
4	31.60269976613235	32.0	32.0	32.0	32.0	32.0
5	31.586554868562366	32.0	32.0	32.0	32.0	32.0
6	35.16744873480613	36.0	36.0	36.0	36.0	36.0
7	35.191203113551275	36.0	36.0	36.0	36.0	36.0
8	35.139399672786034	36.0	36.0	36.0	36.0	36.0
9	35.24132155296149	36.0	36.0	36.0	36.0	36.0
10	35.12534377540676	36.0	36.0	36.0	36.0	36.0
11	35.238893795983095	36.0	36.0	36.0	36.0	36.0
12	35.178657820514104	36.0	36.0	36.0	36.0	36.0
13	35.209923817362416	36.0	36.0	36.0	36.0	36.0
14	35.15851810215901	36.0	36.0	36.0	36.0	36.0
15	35.12981912896847	36.0	36.0	36.0	36.0	36.0
16	35.14314732658162	36.0	36.0	36.0	36.0	36.0
17	35.15039170321894	36.0	36.0	36.0	36.0	36.0
18	35.13917383492758	36.0	36.0	36.0	36.0	36.0
19	35.10054552389364	36.0	36.0	36.0	36.0	36.0
20	35.08175079545112	36.0	36.0	36.0	36.0	36.0
21	35.05737410794046	36.0	36.0	36.0	36.0	36.0
22	35.026493290106295	36.0	36.0	36.0	36.0	36.0
23	35.01897916269359	36.0	36.0	36.0	36.0	36.0
24	34.99887708398157	36.0	36.0	36.0	36.0	36.0
25	34.94176896285218	36.0	36.0	36.0	36.0	36.0
26	34.920118640154975	36.0	36.0	36.0	36.0	36.0
27	34.847171757219286	36.0	36.0	36.0	36.0	36.0
28	34.79214008973291	36.0	36.0	36.0	32.0	36.0
29	34.756935731564106	36.0	36.0	36.0	32.0	36.0
30	34.68977908038824	36.0	36.0	36.0	32.0	36.0
31	34.6815497997571	36.0	36.0	36.0	32.0	36.0
32	34.63662186712704	36.0	36.0	36.0	32.0	36.0
33	34.68333266418412	36.0	36.0	36.0	32.0	36.0
34	34.63928926316635	36.0	36.0	36.0	32.0	36.0
35	34.61545709582551	36.0	36.0	36.0	32.0	36.0
36	34.544168866494694	36.0	36.0	36.0	32.0	36.0
37	34.432342741571226	36.0	36.0	36.0	32.0	36.0
38	34.38672725812766	36.0	36.0	36.0	32.0	36.0
39	34.17855995744211	36.0	36.0	36.0	32.0	36.0
40	34.28227599393751	36.0	36.0	36.0	32.0	36.0
41	34.26907075249174	36.0	36.0	36.0	32.0	36.0
42	34.239317869295085	36.0	36.0	36.0	32.0	36.0
43	34.25212663983378	36.0	36.0	36.0	32.0	36.0
44	34.10933814451616	36.0	36.0	36.0	32.0	36.0
45	34.09377164279477	36.0	36.0	36.0	32.0	36.0
46	33.882241867327785	36.0	36.0	36.0	32.0	36.0
47	33.911809061618605	36.0	36.0	36.0	32.0	36.0
48	33.845067701171345	36.0	36.0	36.0	32.0	36.0
49	33.78317683606179	36.0	36.0	36.0	27.0	36.0
50	33.33178592578466	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	1.0
21	22.0
22	83.0
23	211.0
24	594.0
25	1388.0
26	3005.0
27	5828.0
28	9818.0
29	14893.0
30	21296.0
31	29728.0
32	42359.0
33	67853.0
34	138377.0
35	461571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67295554869617	20.607005101874368	12.495075047492854	26.224964301936605
2	11.520659850519868	21.584660031190836	46.74760893267372	20.14707118561558
3	18.550117310515287	27.34351279123747	30.385807309637023	23.720562588610218
4	10.25642634172781	18.341798070842827	40.831610274116976	30.570165313312387
5	9.91152174567646	41.701336960122056	36.91696192875568	11.470179365445803
6	29.32567324774915	40.08759999598511	19.189317367433176	11.39740938883257
7	26.099077577813688	32.33144967830652	23.33469672484919	18.234776019030605
8	22.21968503146674	43.51243111945317	20.142227664635797	14.125656184444288
9	26.78722437887225	14.36655132004547	20.697650492209714	38.14857380887257
10	14.681829701916877	30.19838750555189	35.98163673456073	19.138146057970506
11	33.78333617721748	20.354515251583376	29.15190356221582	16.71024500898333
12	25.54332961118151	23.98793019083347	34.66268521887507	15.806054979109947
13	30.82999489353587	23.29777210114061	26.27269522188026	19.599537783443264
14	18.607533950958054	25.566602093767877	27.989465918557848	27.836398036716215
15	19.82539369856448	39.20235183997852	22.597814535384877	18.37443992607212
16	18.16304381920266	27.907405664816036	34.3764624698096	19.553088046171702
17	18.482193939515604	31.403381545533932	30.710937578415926	19.40348693653454
18	18.581460445076793	27.22930475728046	37.093161997463085	17.09607280017967
19	24.215589838300094	25.17050758313342	28.19096347449036	22.42293910407612
20	23.972307120954344	29.164345543694825	29.889163241441956	16.974184093908875
21	21.43841150668982	26.36431159602124	27.288866695440085	24.90841020184886
22	23.14401823768461	32.13965328826608	27.613856926518544	17.102471547530772
23	19.304765465618438	31.780519079678704	30.141174817383625	18.773540637319233
24	23.822751521903875	27.462648740069355	30.83756154112528	17.87703819690149
25	21.10170246455238	29.9564252384818	30.861169279103063	18.080703017862767
26	16.928672971212976	31.918553659953503	32.55080672178231	18.60196664705122
27	18.983927025433193	30.97554548990577	30.609417934980364	19.431109549680674
28	17.81472475464179	31.852024722772203	28.547875904331004	21.785374618255002
29	20.65030143723769	28.050287635740855	27.836238966644288	23.463171960377156
30	18.202874256906473	29.16113554979511	30.437035605754538	22.198954587543884
31	22.803697978358194	26.8700688602919	29.657229326855518	20.66900383449439
32	19.40924352456425	31.328261055040574	29.635563830908445	19.62693158948673
33	18.03315712935998	31.538670979544364	32.451100574283586	17.97707131681207
34	20.035683232268482	30.76499379558426	31.76184077298614	17.437482199161117
35	18.63743069400722	31.9340183109601	30.982586143945678	18.445964851087002
36	17.50722739181498	30.494950863773703	32.51033918551309	19.487482558898225
37	18.645405429821103	30.42536693575707	27.898033389961935	23.03119424445989
38	19.833218523635114	33.21526128303288	27.93740299207876	19.014117201253246
39	22.730763053821292	28.122929757292276	28.845130789151423	20.30117639973501
40	23.91523371580209	28.270986116948865	30.21925559744798	17.59452456980107
41	22.93579017630851	26.615157613687124	32.00089835535726	18.448153854647106
42	20.510423776448388	32.220610671698715	30.22844983338017	17.04051571847272
43	17.5341238584081	32.60069031884902	31.331341755507687	18.5338440672352
44	17.380841552419177	32.11421946809832	29.714426236501396	20.790512742981104
45	17.91502826786848	32.18371479690546	29.846628307014395	20.05462862821166
46	19.73669682871869	27.99048465047652	29.908735954069364	22.364082566735423
47	23.09806918028303	28.417641235654358	28.887642578156857	19.59664700590576
48	18.20635263179026	30.019045850564098	28.742421809129965	23.03217970851568
49	21.622120093195875	28.543180880041053	28.843169211737056	20.991529815026016
50	18.906826562478436	33.36608035693001	27.30499523850749	20.422097842084067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	17.5
2	13.0
3	45.0
4	77.0
5	100.0
6	123.0
7	125.5
8	128.0
9	188.0
10	248.0
11	385.0
12	522.0
13	996.0
14	1470.0
15	2013.0
16	2556.0
17	2977.0
18	3398.0
19	3557.5
20	3717.0
21	4249.5
22	4782.0
23	5508.5
24	6235.0
25	7848.5
26	9462.0
27	14352.5
28	19243.0
29	25089.0
30	30935.0
31	38515.5
32	46096.0
33	59227.0
34	72358.0
35	85751.5
36	99145.0
37	96462.0
38	93779.0
39	82727.0
40	71675.0
41	63197.0
42	54719.0
43	48859.5
44	43000.0
45	40798.0
46	38596.0
47	37657.0
48	36718.0
49	35354.0
50	33990.0
51	32124.0
52	30258.0
53	27351.0
54	24444.0
55	21868.5
56	19293.0
57	17138.5
58	14984.0
59	12932.0
60	10880.0
61	9258.0
62	7636.0
63	6452.0
64	5268.0
65	4552.0
66	3836.0
67	3309.5
68	2783.0
69	2303.0
70	1823.0
71	1399.0
72	975.0
73	823.0
74	671.0
75	554.5
76	438.0
77	350.5
78	263.0
79	215.0
80	167.0
81	140.5
82	114.0
83	94.5
84	75.0
85	64.0
86	53.0
87	36.5
88	20.0
89	19.0
90	18.0
91	14.0
92	10.0
93	7.5
94	5.0
95	6.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00878258338435596
2	3.763964307581126E-4
3	2.509309538387417E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.001756516676871192
10	7.527928615162252E-4
11	0.0
12	2.509309538387417E-4
13	3.763964307581126E-4
14	0.0
15	0.004893153599855464
16	8.78258338435596E-4
17	0.0
18	1.2546547691937086E-4
19	0.0
20	5.018619076774835E-4
21	0.0
22	1.2546547691937086E-4
23	7.527928615162252E-4
24	5.018619076774835E-4
25	0.0013801202461130797
26	0.005144084553694205
27	0.005269550030613576
28	0.00426582621525861
29	0.002132913107629305
30	0.0032621023999036425
31	0.007026066707484768
32	0.0025093095383874175
33	0.004893153599855464
34	0.0023838440614680463
35	0.00288570596914553
36	0.008029790522839735
37	0.0032621023999036425
38	0.007653394092081623
39	0.0030111714460649007
40	0.002132913107629305
41	0.0025093095383874175
42	0.001003723815354967
43	0.0011291892922743378
44	6.273273845968544E-4
45	0.001756516676871192
46	5.018619076774835E-4
47	0.0016310511999518213
48	0.001003723815354967
49	1.2546547691937086E-4
50	0.0016310511999518213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	797032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.9282813979571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0918418139843	51.02970240749028
2	12.265557874742704	15.437009548894748
3	3.2600620459298577	6.154503054032213
4	1.198749502921059	3.017409841819104
5	0.5801349426206318	1.8253447459009406
6	0.33610144268843545	1.2690171698254327
7	0.21756347523696415	0.9583626914140404
8	0.14366011973595028	0.7232227552326471
9	0.1181391210900058	0.669086267045329
>10	0.6823974788871099	8.078677284460364
>50	0.061227750603412755	2.7036217335288466
>100	0.03914443313555076	4.7055191450671785
>500	0.00421555432977822	1.807045672503145
>1k	0.0012044440942223487	1.621477682785765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2956	0.3708759497736603	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	2543	0.31905870780596013	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	2271	0.28493209808389125	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1993	0.2500526955003061	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1545	0.19384416184042796	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1528	0.19171124873279868	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	991	0.12433628762709653	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	961	0.12057232331951541	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	917	0.11505184233506309	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	855	0.10727298276606209	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2546547691937086E-4	0.0
3	0.0	0.0	0.0	1.2546547691937086E-4	0.0
4	0.0	0.0	0.0	1.2546547691937086E-4	0.0
5	0.0	0.0	0.0	1.2546547691937086E-4	0.0
6	0.0	0.0	0.0	1.2546547691937086E-4	0.0
7	0.0	0.0	0.0	1.2546547691937086E-4	0.0
8	0.0	0.0	0.0	1.2546547691937086E-4	0.0
9	0.0	0.0	0.0	1.2546547691937086E-4	0.0
10	0.0	0.0	0.0	1.2546547691937086E-4	0.0
11	0.0	0.0	0.0	1.2546547691937086E-4	0.0
12	0.0	0.0	0.0	1.2546547691937086E-4	0.0
13	0.0	0.0	0.0	1.2546547691937086E-4	0.0
14	0.0	0.0	0.0	1.2546547691937086E-4	0.0
15	0.0	0.0	0.0	1.2546547691937086E-4	0.0
16	0.0	0.0	0.0	2.509309538387417E-4	0.0
17	0.0	0.0	0.0	2.509309538387417E-4	0.0
18	0.0	0.0	0.0	2.509309538387417E-4	0.0
19	0.0	0.0	0.0	2.509309538387417E-4	0.0
20	0.0	0.0	0.0	2.509309538387417E-4	0.0
21	0.0	0.0	0.0	3.763964307581126E-4	0.0
22	0.0	0.0	0.0	0.0011291892922743378	0.0
23	0.0	0.0	0.0	0.001881982153790563	0.0
24	0.0	0.0	0.0	0.0032621023999036425	0.0
25	0.0	0.0	0.0	0.003889429784500497	0.0
26	0.0	0.0	0.0	0.004767688122936093	0.0
27	0.0	0.0	0.0	0.005520480984452318	0.0
28	0.0	0.0	0.0	0.006273273845968544	0.0
29	0.0	0.0	0.0	0.009535376245872185	0.0
30	0.0	0.0	0.0	0.015557719138001988	0.0
31	0.0	0.0	0.0	0.03362474781439139	0.0
32	0.0	0.0	0.0	0.05269550030613576	0.0
33	0.0	0.0	0.0	0.07038613255176705	0.0
34	0.0	0.0	0.0	0.08995874695118891	0.0
35	0.0	0.0	0.0	0.11354625661203063	0.0
36	0.0	0.0	0.0	0.1407722651035341	0.0
37	0.0	0.0	0.0	0.18945287014825002	0.0
38	0.0	0.0	0.0	0.2592116753154202	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATA	25	0.002353021	35.19784	11
TCACGCT	40	7.0273795E-4	27.501764	27
TAGGACC	1125	0.0	26.59893	4
CTAGGAC	220	0.0	24.00154	3
AGGACCT	2295	0.0	22.1466	5
GGACCTG	2185	0.0	21.952444	6
GTCCTAT	205	0.0	21.462097	1
TGTAGGA	3670	0.0	20.739874	2
CGCGGTA	85	6.1123683E-6	20.704613	43
TACGGAA	75	5.472219E-5	20.532076	37
GATATAC	870	0.0	20.481504	1
ATAGGAC	215	0.0	20.46643	3
TATAGGA	230	0.0	20.085726	2
CTGTAGG	3545	0.0	20.043905	1
AGGCGTG	55	0.004479004	20.002539	7
AGAGTGT	275	0.0	20.002539	6
CAGTCCG	55	0.004479004	20.002539	9
ATTACGT	55	0.004483886	19.998772	42
CTCTAGG	210	0.0	19.903542	1
TTAGGAC	2365	0.0	19.815224	3
>>END_MODULE