FastQCFastQC Report
Thu 2 Feb 2017
SRR4064122_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064122_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955982
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26070.27270387936174534No Hit
TATCAACGCAGAGTACTTTTTTTTT19220.20104981056128673No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15990.1672625635210705No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9890.10345383072066211No Hit
GGTATCAACGCAGAGTACTTTTTTT9660.1010479276806467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT453.5505647E-516.881121
TTAAGGT755.851507E-815.1969854
TACTTAT456.769128E-414.7748467
CGAACGT400.005284080314.2471744
TCGAACG400.005284080314.2471743
TTGCACC551.9622066E-413.8154414
CGTTATT551.96744E-413.8111052
TCTTATG500.001504608113.2931872
GTATAAA957.419476E-812.994021
GCGCCGA751.4714482E-512.67144919
TCGCACG604.0836647E-412.66879713
CTCTTAT751.4841753E-512.6608411
ATTAACC1457.2759576E-1212.4458073
CGGACAT701.0920725E-412.2118635
GTATTAG701.0947435E-412.2086691
GTATAAT1103.8309736E-812.0853491
TGAGTTA802.8587285E-511.87699612
CGCGGTC802.8633276E-511.87513110
GTCCTAG802.8771648E-511.8695391
AACCCGG658.0177136E-411.69243711
Produced by FastQC (version 0.11.5)