##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064122_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 955982 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.211673441550154 32.0 32.0 32.0 32.0 32.0 2 30.754226544014426 32.0 32.0 32.0 32.0 32.0 3 30.774458096491358 32.0 32.0 32.0 32.0 32.0 4 30.80799638486917 32.0 32.0 32.0 32.0 32.0 5 30.689871775828415 32.0 32.0 32.0 32.0 32.0 6 34.35706843852709 36.0 36.0 36.0 32.0 36.0 7 34.27405013901935 36.0 36.0 36.0 32.0 36.0 8 34.22802521386386 36.0 36.0 36.0 32.0 36.0 9 34.402323474709775 36.0 36.0 36.0 32.0 36.0 10 34.05432529064355 36.0 36.0 36.0 32.0 36.0 11 34.385088840584864 36.0 36.0 36.0 32.0 36.0 12 34.16810149145068 36.0 36.0 36.0 32.0 36.0 13 34.273740509758554 36.0 36.0 36.0 32.0 36.0 14 34.14923293534815 36.0 36.0 36.0 32.0 36.0 15 34.077110238477296 36.0 36.0 36.0 32.0 36.0 16 34.07738116408049 36.0 36.0 36.0 32.0 36.0 17 34.00415698203523 36.0 36.0 36.0 32.0 36.0 18 33.998671523103994 36.0 36.0 36.0 32.0 36.0 19 34.01242805826888 36.0 36.0 36.0 32.0 36.0 20 33.99855855026559 36.0 36.0 36.0 32.0 36.0 21 33.97133000412142 36.0 36.0 36.0 32.0 36.0 22 33.95554623413412 36.0 36.0 36.0 32.0 36.0 23 33.89528150111613 36.0 36.0 36.0 32.0 36.0 24 33.87309698299759 36.0 36.0 36.0 32.0 36.0 25 33.472367680563025 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 12.0 5 10.0 6 44.0 7 10.0 8 44.0 9 68.0 10 77.0 11 25.0 12 61.0 13 54.0 14 90.0 15 195.0 16 289.0 17 402.0 18 466.0 19 614.0 20 1017.0 21 1583.0 22 2315.0 23 3698.0 24 5588.0 25 8146.0 26 11546.0 27 15134.0 28 20685.0 29 27995.0 30 36803.0 31 49725.0 32 69910.0 33 97231.0 34 203006.0 35 399136.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.95174958507657 16.859286017760606 10.986942207915881 26.202022189246943 2 16.74536739676675 19.90800697113423 37.24158997093966 26.10503566115936 3 18.904372968019718 24.025381708934646 28.561745002949902 28.508500320095738 4 12.69049233009465 15.250893357769913 35.77845564175307 36.28015867038237 5 14.600662206623113 36.60224187803054 33.781116126771245 15.015979788575104 6 34.38809030611177 35.186324357123425 16.903521436193586 13.522063900571213 7 30.26037007392062 30.39387709193256 20.964891630177192 18.380861203969637 8 28.298372396242094 32.71720044619406 19.653257823569177 19.331169333994673 9 27.80621455504845 13.830634484909437 18.28177212460925 40.081378835432865 10 15.746771426747314 26.84883505520418 32.311688801018995 25.092704717029513 11 37.309388599214216 20.8071771406563 22.55530739587934 19.328126864250144 12 24.830752934871803 23.568464990873007 29.084223703381173 22.516558370874016 13 29.840070335559233 19.483787234933327 24.983672102321492 25.69247032718595 14 23.48272256350621 19.439196381750072 25.607847775594816 31.4702332791489 15 25.235782160414555 27.036319506152683 22.46312420389832 25.264774129534445 16 25.536798738939027 25.54988245588694 23.949900877760403 24.96341792741363 17 24.135289081291095 25.623452228686023 25.334674473469914 24.906584216552965 18 24.695538755608222 24.887835577319652 26.222189189924777 24.19443647714735 19 25.587042924030108 24.69563160302259 25.535961580130195 24.181363892817103 20 25.774844863538537 24.35370275770239 25.141842041352973 24.7296103374061 21 26.669772164619303 24.140082382928835 24.732988239235002 24.45715721321686 22 25.83688282541314 24.29087795365021 25.127682269776752 24.744556951159897 23 24.631747543849546 24.231121532993807 25.420123422541625 25.71700750061502 24 24.758633756624945 24.959096233503715 25.267379259304235 25.014890750567105 25 24.950984035398267 24.597273529494892 25.285433596321997 25.166308838784847 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 248.0 1 248.0 2 378.5 3 509.0 4 509.0 5 509.0 6 1327.5 7 2146.0 8 2146.0 9 2146.0 10 2153.0 11 2160.0 12 2160.0 13 2160.0 14 2392.0 15 2624.0 16 2624.0 17 2624.0 18 4383.5 19 6143.0 20 6143.0 21 6143.0 22 9800.0 23 13457.0 24 13457.0 25 13457.0 26 20017.5 27 26578.0 28 26578.0 29 26578.0 30 33227.5 31 39877.0 32 39877.0 33 39877.0 34 49058.5 35 58240.0 36 58240.0 37 58240.0 38 66984.5 39 75729.0 40 75729.0 41 75729.0 42 87340.0 43 98951.0 44 98951.0 45 98951.0 46 112250.0 47 125549.0 48 125549.0 49 125549.0 50 128671.5 51 131794.0 52 131794.0 53 131794.0 54 123298.0 55 114802.0 56 114802.0 57 114802.0 58 105131.0 59 95460.0 60 95460.0 61 95460.0 62 83258.0 63 71056.0 64 71056.0 65 71056.0 66 58123.5 67 45191.0 68 45191.0 69 45191.0 70 33948.0 71 22705.0 72 22705.0 73 22705.0 74 17095.5 75 11486.0 76 11486.0 77 11486.0 78 8935.5 79 6385.0 80 6385.0 81 6385.0 82 4359.5 83 2334.0 84 2334.0 85 2334.0 86 1791.0 87 1248.0 88 1248.0 89 1248.0 90 889.0 91 530.0 92 530.0 93 530.0 94 389.5 95 249.0 96 249.0 97 249.0 98 390.0 99 531.0 100 531.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04016812032025707 2 0.004184179200026779 3 0.0018828806400120503 4 0.00397497024002544 5 0.009100589760058245 6 0.012761746560081675 7 0.02374521696015197 8 0.035983941120230296 9 0.04717662048030193 10 0.055335769920354154 11 0.05272065792033741 12 0.06004297152038428 13 0.058787717760376244 14 0.06307650144040369 15 0.057009441600364856 16 0.062344270080399 17 0.06025218048038561 18 0.07196788224046059 19 0.06726068064043048 20 0.07259550912046461 21 0.07186327776045993 22 0.0712356508804559 23 0.0760474569604867 24 0.0722816956804626 25 0.0712356508804559 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 955982.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.211693677683506 #Duplication Level Percentage of deduplicated Percentage of total 1 78.36702525665477 46.40244293928329 2 13.274936470806672 15.720629438002273 3 3.928912369676056 6.979126671591608 4 1.572222132701703 3.7237574125923003 5 0.8034422775177882 2.378658901204183 6 0.4691781105834301 1.666849833848423 7 0.3058039560410459 1.2675019119368343 8 0.21509235531882534 1.0188786124439797 9 0.16228402799379676 0.8648200938914379 >10 0.7702579380424688 8.052504117994802 >50 0.06569428957616098 2.686345671374224 >100 0.06018018085615693 6.861537706171444 >500 0.004438066277756269 1.7328070658086663 >1k 5.325679533307524E-4 0.6441396238565669 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2607 0.27270387936174534 No Hit TATCAACGCAGAGTACTTTTTTTTT 1922 0.20104981056128673 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1599 0.1672625635210705 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 989 0.10345383072066211 No Hit GGTATCAACGCAGAGTACTTTTTTT 966 0.1010479276806467 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.0460448000066946E-4 0.0 6 0.0 0.0 0.0 1.0460448000066946E-4 1.0460448000066946E-4 7 0.0 0.0 0.0 1.0460448000066946E-4 3.1381344000200843E-4 8 0.0 0.0 0.0 1.0460448000066946E-4 3.1381344000200843E-4 9 0.0 0.0 0.0 1.0460448000066946E-4 3.1381344000200843E-4 10 0.0 0.0 0.0 1.0460448000066946E-4 3.1381344000200843E-4 11 0.0 0.0 0.0 1.0460448000066946E-4 3.1381344000200843E-4 12 0.0 0.0 0.0 1.0460448000066946E-4 5.230224000033473E-4 13 0.0 0.0 0.0 1.0460448000066946E-4 5.230224000033473E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTAT 45 3.5505647E-5 16.88112 1 TTAAGGT 75 5.851507E-8 15.196985 4 TACTTAT 45 6.769128E-4 14.774846 7 CGAACGT 40 0.0052840803 14.247174 4 TCGAACG 40 0.0052840803 14.247174 3 TTGCACC 55 1.9622066E-4 13.815441 4 CGTTATT 55 1.96744E-4 13.811105 2 TCTTATG 50 0.0015046081 13.293187 2 GTATAAA 95 7.419476E-8 12.99402 1 GCGCCGA 75 1.4714482E-5 12.671449 19 TCGCACG 60 4.0836647E-4 12.668797 13 CTCTTAT 75 1.4841753E-5 12.660841 1 ATTAACC 145 7.2759576E-12 12.445807 3 CGGACAT 70 1.0920725E-4 12.211863 5 GTATTAG 70 1.0947435E-4 12.208669 1 GTATAAT 110 3.8309736E-8 12.085349 1 TGAGTTA 80 2.8587285E-5 11.876996 12 CGCGGTC 80 2.8633276E-5 11.875131 10 GTCCTAG 80 2.8771648E-5 11.869539 1 AACCCGG 65 8.0177136E-4 11.692437 11 >>END_MODULE