FastQCFastQC Report
Thu 2 Feb 2017
SRR4064122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955982
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1105.2496034E-922.0002351
ACCGTGT1306.91216E-1121.9990848
GTATCAA20700.020.6185761
GGATCGT755.4728534E-520.5324786
TAACGGC851.4297459E-418.11878836
TTAACGG851.4297459E-418.11878835
TAATACC1002.4830204E-517.5992684
AACGCAG24200.017.5447246
TTATACT1551.4386387E-817.031554
TAGGACG1702.4665496E-916.8228284
TAAGACC1053.774358E-516.7612084
CCTATAG1053.776051E-516.760333
ACGAACG1105.6187575E-516.00017215
GTATAAG1805.2605174E-915.8890591
TTACACT1805.2641553E-915.8882284
GTTTAAG1401.5851056E-615.7144541
CTAGGAC1401.5868336E-615.712813
GTATAGC1158.2139144E-515.3045111
ATAGACT1158.2175815E-515.3037124
GTAGGAC2303.6379788E-1115.3029113