##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064122_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	955982
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.283639231701017	32.0	32.0	32.0	32.0	32.0
2	31.396327545916137	32.0	32.0	32.0	32.0	32.0
3	31.466434514457386	32.0	32.0	32.0	32.0	32.0
4	31.547190219062703	32.0	32.0	32.0	32.0	32.0
5	31.503926852179227	32.0	32.0	32.0	32.0	32.0
6	35.07970861376051	36.0	36.0	36.0	36.0	36.0
7	35.09628110152702	36.0	36.0	36.0	36.0	36.0
8	35.044963189683486	36.0	36.0	36.0	36.0	36.0
9	35.147755920090546	36.0	36.0	36.0	36.0	36.0
10	35.008619409152054	36.0	36.0	36.0	36.0	36.0
11	35.15499036592739	36.0	36.0	36.0	36.0	36.0
12	35.05677721965476	36.0	36.0	36.0	36.0	36.0
13	35.09594532114622	36.0	36.0	36.0	36.0	36.0
14	35.03714609689304	36.0	36.0	36.0	36.0	36.0
15	35.00888196639686	36.0	36.0	36.0	36.0	36.0
16	35.025711781184164	36.0	36.0	36.0	36.0	36.0
17	34.987919228604724	36.0	36.0	36.0	36.0	36.0
18	34.982130416681485	36.0	36.0	36.0	36.0	36.0
19	34.97410411493104	36.0	36.0	36.0	36.0	36.0
20	34.97171913278702	36.0	36.0	36.0	36.0	36.0
21	34.97311978677423	36.0	36.0	36.0	36.0	36.0
22	34.95161833591009	36.0	36.0	36.0	36.0	36.0
23	34.89813197319615	36.0	36.0	36.0	32.0	36.0
24	34.87134171982318	36.0	36.0	36.0	32.0	36.0
25	34.844891431010204	36.0	36.0	36.0	32.0	36.0
26	34.786896615208235	36.0	36.0	36.0	32.0	36.0
27	34.783556594161816	36.0	36.0	36.0	32.0	36.0
28	34.763746597739285	36.0	36.0	36.0	32.0	36.0
29	34.72589232851664	36.0	36.0	36.0	32.0	36.0
30	34.70856878058373	36.0	36.0	36.0	32.0	36.0
31	34.691092510110025	36.0	36.0	36.0	32.0	36.0
32	34.659982091713026	36.0	36.0	36.0	32.0	36.0
33	34.62615718706001	36.0	36.0	36.0	32.0	36.0
34	34.606480038327085	36.0	36.0	36.0	32.0	36.0
35	34.569830812714045	36.0	36.0	36.0	32.0	36.0
36	34.543377385766675	36.0	36.0	36.0	32.0	36.0
37	34.53254663790741	36.0	36.0	36.0	32.0	36.0
38	34.48541395130871	36.0	36.0	36.0	32.0	36.0
39	34.491737292124746	36.0	36.0	36.0	32.0	36.0
40	34.45643223407972	36.0	36.0	36.0	32.0	36.0
41	34.43222675740757	36.0	36.0	36.0	32.0	36.0
42	34.37083020391597	36.0	36.0	36.0	32.0	36.0
43	34.353391591055065	36.0	36.0	36.0	32.0	36.0
44	34.29192181442747	36.0	36.0	36.0	32.0	36.0
45	34.25211562560801	36.0	36.0	36.0	32.0	36.0
46	34.24143341611035	36.0	36.0	36.0	32.0	36.0
47	34.18020213769715	36.0	36.0	36.0	32.0	36.0
48	34.135357151076064	36.0	36.0	36.0	32.0	36.0
49	34.11269877466312	36.0	36.0	36.0	32.0	36.0
50	33.51798360220172	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	9.0
21	35.0
22	139.0
23	349.0
24	918.0
25	2032.0
26	4058.0
27	7471.0
28	11958.0
29	17694.0
30	25035.0
31	35119.0
32	49982.0
33	79142.0
34	162964.0
35	559074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99490316857205	17.762645832984617	11.538904139397085	26.703546859046245
2	15.990903584903885	20.669971474328463	36.809518180800666	26.52960675996699
3	18.175380238080294	24.51097303290864	28.768279671122826	28.54536705788824
4	12.272302198158542	16.019653089702526	35.80747336246917	35.90057134966976
5	14.226732302491051	37.08322960055733	33.40355780757378	15.286480289377833
6	34.36072474243735	35.555099944454966	16.705039116886216	13.379136196221472
7	30.075043253952483	30.636037080196072	20.93292551533397	18.35599415051748
8	28.16873121042028	33.07217081493166	19.3905324577241	19.368565516923958
9	27.745675623655824	13.68182883252299	18.182046411206976	40.39044913261421
10	15.559958283340638	27.162796438811554	32.20812721698207	25.069118060865737
11	37.51357243128008	20.805935676613156	22.385044906703264	19.29544698540349
12	24.725177592441693	23.676830397256847	29.15444972232712	22.443542287974342
13	29.87697402761782	19.525678965079702	25.026648130655865	25.570698876646613
14	23.59808782610515	19.267767108098496	25.561518023389613	31.572627042406744
15	25.191484910298655	27.064804644594382	22.378890109315343	25.36482033579162
16	25.544810272235154	25.510708962054444	23.941421062266272	25.00305970344413
17	24.222631806875025	25.590963009763783	25.33876160848217	24.847643574879026
18	24.74319546434026	24.82698382811356	26.313835017468985	24.1159856900772
19	25.595932969308983	24.768928220255653	25.56486537375259	24.070273436682776
20	25.818538618285892	24.256045156134277	25.220822367182304	24.704593858397526
21	26.71744865489099	24.07639474383409	24.77630331951857	24.42985328175635
22	25.81170546276547	24.24451950404872	25.20866000474905	24.73511502843676
23	24.62033486266363	24.061114632824744	25.54556923096235	25.772981273549277
24	24.87180667337533	24.77379186550318	25.401839791292268	24.952561669829223
25	24.96514008299432	24.36428377907652	25.43638967372373	25.234186464205425
26	24.833905376015633	25.066558151163633	25.651223780795895	24.448312692024842
27	25.232315222701455	24.864607012504173	25.002484514933766	24.900593249860606
28	24.645531404756376	24.66530256146319	25.75177469668535	24.937391337095082
29	24.701556550483282	24.784823632787983	25.589669023808526	24.923950792920206
30	24.651656150099587	24.99926774566087	25.73947227475857	24.609603829480978
31	25.15074609378269	24.710642820678057	24.979182184134103	25.15942890140515
32	24.87923059059327	24.84889440286876	24.86563160988918	25.406243396648794
33	24.65262050143157	24.597387082702628	25.318769006101828	25.43122340976397
34	25.17749229306339	24.673808549966157	25.463802242151058	24.68489691481939
35	25.633166622905616	24.450362937771786	25.4804388100202	24.4360316293024
36	24.7503177583781	25.087690851174006	25.237390353744843	24.924601036703052
37	25.418280265038106	24.91458651084066	25.02955212576548	24.637581098355753
38	24.770872541214697	24.90456727575533	25.445309124613324	24.87925105841665
39	25.166564672284203	24.791016125573393	24.96236668811164	25.080052514030765
40	25.380900226892578	24.831713493988758	25.375879091048887	24.41150718806978
41	24.581148765414017	25.210157874696115	25.523250218631137	24.685443141258727
42	25.483099241288627	25.271689385467848	25.073146136409047	24.17206523683448
43	24.916550117525468	24.526158439186453	25.40893319092894	25.148358252359138
44	24.71894832749803	25.127541547110088	24.819370624176877	25.334139501215002
45	24.915661138221587	25.087843174174786	25.064829779332925	24.931665908270702
46	24.653467521504314	25.02283019045557	25.03151246219812	25.292189825841998
47	24.776037591373733	24.831583616119435	25.46361578469482	24.92876300781201
48	25.314212609091797	25.298626199185946	24.611987309105707	24.77517388261655
49	24.619160044310597	25.512275897273895	24.709015574609904	25.159548483805604
50	24.709848770930375	25.60632323082282	24.761733407115518	24.922094591131284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	126.0
1	86.5
2	47.0
3	128.0
4	209.0
5	229.0
6	249.0
7	222.0
8	195.0
9	280.0
10	365.0
11	588.5
12	812.0
13	1412.5
14	2013.0
15	2584.5
16	3156.0
17	3235.0
18	3314.0
19	3295.5
20	3277.0
21	3517.5
22	3758.0
23	4250.0
24	4742.0
25	5445.5
26	6149.0
27	7233.0
28	8317.0
29	9700.0
30	11083.0
31	12867.0
32	14651.0
33	17250.5
34	19850.0
35	22765.5
36	25681.0
37	29500.0
38	33319.0
39	36466.5
40	39614.0
41	43747.5
42	47881.0
43	50072.5
44	52264.0
45	56974.5
46	61685.0
47	65369.0
48	69053.0
49	72145.5
50	75238.0
51	73465.0
52	71692.0
53	71244.0
54	70796.0
55	70986.5
56	71177.0
57	68207.5
58	65238.0
59	59913.5
60	54589.0
61	48025.5
62	41462.0
63	35541.5
64	29621.0
65	25201.5
66	20782.0
67	17642.0
68	14502.0
69	12836.0
70	11170.0
71	8803.5
72	6437.0
73	5433.5
74	4430.0
75	3510.5
76	2591.0
77	2144.0
78	1697.0
79	1432.0
80	1167.0
81	950.0
82	733.0
83	584.5
84	436.0
85	314.5
86	193.0
87	138.0
88	83.0
89	65.0
90	47.0
91	40.0
92	33.0
93	25.5
94	18.0
95	20.0
96	22.0
97	16.5
98	11.0
99	9.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00983282112006293
2	1.0460448000066946E-4
3	2.0920896000133893E-4
4	0.0
5	0.0
6	1.0460448000066946E-4
7	0.0
8	0.0
9	6.276268800040168E-4
10	9.414403200060251E-4
11	0.0
12	1.0460448000066946E-4
13	5.230224000033473E-4
14	2.0920896000133893E-4
15	0.003347343360021423
16	7.322313600046863E-4
17	0.0
18	2.0920896000133893E-4
19	2.0920896000133893E-4
20	0.0010460448000066947
21	0.0
22	1.0460448000066946E-4
23	8.368358400053557E-4
24	4.1841792000267786E-4
25	0.001359858240008703
26	0.005334828480034143
27	0.006380873280040837
28	0.004602597120029457
29	0.0023012985600147284
30	0.003138134400020084
31	0.007322313600046863
32	0.002928925440018745
33	0.00407957472002611
34	0.0026151120000167368
35	0.0034519478400220926
36	0.007008500160044854
37	0.004602597120029457
38	0.007636127040048871
39	0.004916410560031465
40	0.0024059030400153977
41	0.003556552320022762
42	0.00198748512001272
43	0.00198748512001272
44	0.00198748512001272
45	0.001778276160011381
46	0.0011506492800073642
47	0.0018828806400120503
48	0.0021966940800140587
49	3.138134400020084E-4
50	0.001569067200010042
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	955982.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.81456073762277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75967060356743	50.10068673540571
2	12.60131007035551	15.830915135759275
3	3.682434403339807	6.939314984727001
4	1.4546891384730003	3.6550263697188967
5	0.726761430697736	2.2825600015162277
6	0.4282640411397447	1.6140730574347364
7	0.2583072675431265	1.1357820282239965
8	0.18947192745339053	0.9521276716076363
9	0.1371412891025863	0.7753022850573261
>10	0.6564359068715384	7.287753453838591
>50	0.054859431527915656	2.4142952981790295
>100	0.04763534837424867	5.819605397503663
>500	0.0030191415540328503	1.1925575810279652
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.0460448000066946E-4	0.0
6	0.0	0.0	0.0	1.0460448000066946E-4	2.0920896000133893E-4
7	0.0	0.0	0.0	1.0460448000066946E-4	2.0920896000133893E-4
8	0.0	0.0	0.0	1.0460448000066946E-4	3.1381344000200843E-4
9	0.0	0.0	0.0	1.0460448000066946E-4	4.1841792000267786E-4
10	0.0	0.0	0.0	1.0460448000066946E-4	4.1841792000267786E-4
11	0.0	0.0	0.0	1.0460448000066946E-4	4.1841792000267786E-4
12	0.0	0.0	0.0	1.0460448000066946E-4	7.322313600046863E-4
13	0.0	0.0	0.0	1.0460448000066946E-4	8.368358400053557E-4
14	0.0	0.0	0.0	1.0460448000066946E-4	8.368358400053557E-4
15	0.0	0.0	0.0	1.0460448000066946E-4	8.368358400053557E-4
16	0.0	0.0	0.0	1.0460448000066946E-4	8.368358400053557E-4
17	0.0	0.0	0.0	1.0460448000066946E-4	9.414403200060252E-4
18	0.0	0.0	0.0	1.0460448000066946E-4	9.414403200060252E-4
19	0.0	0.0	0.0	1.0460448000066946E-4	9.414403200060252E-4
20	0.0	0.0	0.0	3.1381344000200843E-4	9.414403200060252E-4
21	0.0	0.0	0.0	5.230224000033473E-4	9.414403200060252E-4
22	0.0	0.0	0.0	0.0010460448000066947	0.0010460448000066947
23	0.0	0.0	0.0	0.0015690672000100421	0.0010460448000066947
24	0.0	0.0	0.0	0.0024059030400153977	0.0010460448000066947
25	0.0	0.0	0.0	0.002928925440018745	0.0012552537600080337
26	0.0	0.0	0.0	0.003556552320022762	0.0012552537600080337
27	0.0	0.0	0.0	0.004602597120029457	0.0012552537600080337
28	0.0	0.0	0.0	0.008263753920052888	0.0012552537600080337
29	0.0	0.0	0.0	0.01579527648010109	0.0012552537600080337
30	0.0	0.0	0.0	0.02772018720017741	0.0012552537600080337
31	0.0	0.0	0.0	0.054185120640346786	0.0012552537600080337
32	0.0	0.0	0.0	0.08441581536054026	0.0012552537600080337
33	0.0	0.0	0.0	0.11349586080072638	0.0012552537600080337
34	0.0	0.0	0.0	0.14299432416091518	0.0012552537600080337
35	0.0	0.0	0.0	0.17489869056111934	0.0012552537600080337
36	0.0	0.0	0.0	0.2213430796814166	0.0012552537600080337
37	0.0	0.0	0.0	0.29331096192187717	0.0012552537600080337
38	0.0	0.0	0.0	0.39530032992252995	0.001359858240008703
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	110	5.2496034E-9	22.000235	1
ACCGTGT	130	6.91216E-11	21.999084	8
GTATCAA	2070	0.0	20.618576	1
GGATCGT	75	5.4728534E-5	20.532478	6
TAACGGC	85	1.4297459E-4	18.118788	36
TTAACGG	85	1.4297459E-4	18.118788	35
TAATACC	100	2.4830204E-5	17.599268	4
AACGCAG	2420	0.0	17.544724	6
TTATACT	155	1.4386387E-8	17.03155	4
TAGGACG	170	2.4665496E-9	16.822828	4
TAAGACC	105	3.774358E-5	16.761208	4
CCTATAG	105	3.776051E-5	16.76033	3
ACGAACG	110	5.6187575E-5	16.000172	15
GTATAAG	180	5.2605174E-9	15.889059	1
TTACACT	180	5.2641553E-9	15.888228	4
GTTTAAG	140	1.5851056E-6	15.714454	1
CTAGGAC	140	1.5868336E-6	15.71281	3
GTATAGC	115	8.2139144E-5	15.304511	1
ATAGACT	115	8.2175815E-5	15.303712	4
GTAGGAC	230	3.6379788E-11	15.302911	3
>>END_MODULE