FastQCFastQC Report
Thu 2 Feb 2017
SRR4064121_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064121_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT240.6609749380335995No Hit
TATCAACGCAGAGTACTTTTTTTTT220.6058936931974662No Hit
GTACTTTTTTTTTTTTTTTTTTTTT110.3029468465987331No Hit
GAGTACTTTTTTTTTTTTTTTTTTT110.3029468465987331No Hit
GTCCTAAAGTGTGTATTTCTCATTT110.3029468465987331No Hit
CTTTAGGACGTGAAATATGGCGAGG100.27540622418066646No Hit
ACGCAGAGTACTTTTTTTTTTTTTT100.27540622418066646No Hit
CTGTAGGACGTGGAATATGGCAAGA80.2203249793445332No Hit
GATATACACTGTTCTACAAATCCCG70.19278435692646653No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG60.16524373450839988No Hit
GGTATCAACGCAGAGTACTTTTTTT60.16524373450839988No Hit
GTCTTGCGCCGGTCCAAGAATTTCA50.13770311209033323No Hit
GTCCTACAGTGGACATTTCTAAATT50.13770311209033323No Hit
GTACATGGGTACCTGGTTGATCCTG50.13770311209033323No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG50.13770311209033323No Hit
CTGAAGGACCTGGAATATGGCGAGA40.1101624896722666No Hit
GTTCTAAGTTGCTTGTTAAGCGCAC40.1101624896722666No Hit
GTATCAACGCAGAGTACATGGGAAA40.1101624896722666No Hit
ACAGTGGACATTTCTAAATTTTCCA40.1101624896722666No Hit
ATTTAGAAATGTCCACTGTAGGACG40.1101624896722666No Hit
CTGTAGAACATATTAGATGAGTGAG40.1101624896722666No Hit
GCAGAGTACTTTTTTTTTTTTTTTT40.1101624896722666No Hit
TTGTAGAACAGTGTATATCAATGAG40.1101624896722666No Hit
GTGAAATATGGCGAGGAAAACTGAA40.1101624896722666No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)