##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064121_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3631
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.754062241806665	32.0	32.0	32.0	14.0	32.0
2	27.22693472872487	32.0	21.0	32.0	14.0	32.0
3	27.570641696502342	32.0	21.0	32.0	14.0	32.0
4	27.21481685486092	32.0	21.0	32.0	14.0	32.0
5	27.215918479757644	32.0	21.0	32.0	14.0	32.0
6	30.807766455521893	36.0	21.0	36.0	14.0	36.0
7	30.602038006058937	36.0	21.0	36.0	14.0	36.0
8	30.445331864500137	36.0	21.0	36.0	14.0	36.0
9	30.436794271550536	36.0	21.0	36.0	14.0	36.0
10	29.543927292756816	36.0	21.0	36.0	14.0	36.0
11	31.325530156981547	36.0	32.0	36.0	14.0	36.0
12	30.10217570917103	36.0	21.0	36.0	14.0	36.0
13	30.54833379234371	36.0	21.0	36.0	14.0	36.0
14	30.093638116221427	36.0	21.0	36.0	14.0	36.0
15	29.986780501239327	36.0	21.0	36.0	14.0	36.0
16	30.03717984026439	36.0	21.0	36.0	14.0	36.0
17	29.746075461305427	36.0	21.0	36.0	14.0	36.0
18	29.785733957587443	36.0	21.0	36.0	14.0	36.0
19	29.936656568438448	36.0	21.0	36.0	14.0	36.0
20	29.787661801156705	36.0	21.0	36.0	14.0	36.0
21	30.047920683007437	36.0	21.0	36.0	14.0	36.0
22	29.572018727623245	36.0	21.0	36.0	14.0	36.0
23	29.349215092261087	36.0	21.0	36.0	14.0	36.0
24	29.51198017075186	36.0	21.0	36.0	14.0	36.0
25	28.62076562930322	36.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0
15	13.0
16	28.0
17	69.0
18	69.0
19	129.0
20	140.0
21	131.0
22	101.0
23	91.0
24	135.0
25	106.0
26	114.0
27	125.0
28	121.0
29	132.0
30	182.0
31	173.0
32	231.0
33	301.0
34	521.0
35	713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24731478931424	19.08565133572019	11.759845772514458	26.907188102451112
2	14.844395483337925	19.829248141007987	41.696502340952904	23.629854034701182
3	19.28374655647383	24.40771349862259	28.81542699724518	27.493112947658403
4	11.212121212121213	14.325068870523417	37.52066115702479	36.94214876033058
5	13.164417515835858	38.72211511980171	34.04020930873038	14.073258055632056
6	33.50785340314136	37.03499586662993	16.423257095618627	13.033893634610086
7	28.34298318169286	30.962227736421283	21.80865729252826	18.886131789357595
8	27.11490768806834	36.07054284926977	17.966381923394874	18.848167539267017
9	25.98511986773216	13.99834665197024	19.399283549187103	40.6172499311105
10	15.596583080738496	25.68200606227611	34.720308624965554	24.001102232019843
11	36.97988426563792	20.94240837696335	24.414439239459906	17.663268117938827
12	25.027563395810365	23.84233737596472	30.264608599779493	20.865490628445425
13	28.921974083264406	21.064240419079127	25.50317066446099	24.51061483319548
14	22.27122381477398	20.97574421168688	27.23263506063947	29.52039691289967
15	22.953981813171673	30.283824745108845	23.86332322953982	22.898870212179663
16	23.48401323042999	26.323042998897467	26.295479603087102	23.897464167585447
17	20.914852576467347	27.032240286580322	27.721135298980435	24.331771837971893
18	21.25137816979052	26.405733186328558	30.59536934950386	21.747519294377067
19	25.744211686879826	24.28335170893054	26.681367144432194	23.29106945975744
20	25.475599669148057	25.64102564102564	27.377998345740277	21.50537634408602
21	25.44101433296582	24.448732083792724	26.157662624035282	23.952590959206173
22	23.793768955059278	26.082161566032536	27.267714364488562	22.856355114419628
23	21.367521367521366	26.1373035566584	27.874276261373037	24.6208988144472
24	23.594266813671442	24.972436604189635	28.858875413450935	22.574421168687984
25	23.594266813671442	24.91730981256891	28.5832414553473	22.90518191841235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	9.5
7	16.0
8	16.0
9	16.0
10	16.5
11	17.0
12	17.0
13	17.0
14	16.5
15	16.0
16	16.0
17	16.0
18	25.0
19	34.0
20	34.0
21	34.0
22	46.5
23	59.0
24	59.0
25	59.0
26	102.0
27	145.0
28	145.0
29	145.0
30	204.0
31	263.0
32	263.0
33	263.0
34	289.5
35	316.0
36	316.0
37	316.0
38	323.0
39	330.0
40	330.0
41	330.0
42	365.5
43	401.0
44	401.0
45	401.0
46	450.5
47	500.0
48	500.0
49	500.0
50	464.5
51	429.0
52	429.0
53	429.0
54	385.5
55	342.0
56	342.0
57	342.0
58	300.0
59	258.0
60	258.0
61	258.0
62	233.5
63	209.0
64	209.0
65	209.0
66	163.0
67	117.0
68	117.0
69	117.0
70	97.5
71	78.0
72	78.0
73	78.0
74	58.5
75	39.0
76	39.0
77	39.0
78	34.0
79	29.0
80	29.0
81	29.0
82	20.0
83	11.0
84	11.0
85	11.0
86	10.5
87	10.0
88	10.0
89	10.0
90	5.5
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	4.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.02754062241806665
4	0.02754062241806665
5	0.0
6	0.0550812448361333
7	0.1101624896722666
8	0.0550812448361333
9	0.0550812448361333
10	0.0550812448361333
11	0.0550812448361333
12	0.08262186725419994
13	0.1101624896722666
14	0.08262186725419994
15	0.0550812448361333
16	0.08262186725419994
17	0.0550812448361333
18	0.08262186725419994
19	0.08262186725419994
20	0.1101624896722666
21	0.08262186725419994
22	0.1101624896722666
23	0.1101624896722666
24	0.08262186725419994
25	0.08262186725419994
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3631.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.55356651060315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.43430656934306	85.51363260809694
2	4.045012165450122	7.325805563205728
3	0.7907542579075426	2.1481685486091986
4	0.2737226277372263	0.9914624070503993
5	0.12165450121654502	0.5508124483613329
6	0.06082725060827251	0.33048746901679976
7	0.030413625304136254	0.19278435692646653
8	0.030413625304136254	0.2203249793445332
9	0.0	0.0
>10	0.21289537712895376	2.726521619388598
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	24	0.6609749380335995	No Hit
TATCAACGCAGAGTACTTTTTTTTT	22	0.6058936931974662	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	11	0.3029468465987331	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	11	0.3029468465987331	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	11	0.3029468465987331	No Hit
CTTTAGGACGTGAAATATGGCGAGG	10	0.27540622418066646	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	10	0.27540622418066646	No Hit
CTGTAGGACGTGGAATATGGCAAGA	8	0.2203249793445332	No Hit
GATATACACTGTTCTACAAATCCCG	7	0.19278435692646653	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	6	0.16524373450839988	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6	0.16524373450839988	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	5	0.13770311209033323	No Hit
GTCCTACAGTGGACATTTCTAAATT	5	0.13770311209033323	No Hit
GTACATGGGTACCTGGTTGATCCTG	5	0.13770311209033323	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	5	0.13770311209033323	No Hit
CTGAAGGACCTGGAATATGGCGAGA	4	0.1101624896722666	No Hit
GTTCTAAGTTGCTTGTTAAGCGCAC	4	0.1101624896722666	No Hit
GTATCAACGCAGAGTACATGGGAAA	4	0.1101624896722666	No Hit
ACAGTGGACATTTCTAAATTTTCCA	4	0.1101624896722666	No Hit
ATTTAGAAATGTCCACTGTAGGACG	4	0.1101624896722666	No Hit
CTGTAGAACATATTAGATGAGTGAG	4	0.1101624896722666	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4	0.1101624896722666	No Hit
TTGTAGAACAGTGTATATCAATGAG	4	0.1101624896722666	No Hit
GTGAAATATGGCGAGGAAAACTGAA	4	0.1101624896722666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE