##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064121_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3631
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.085375929496006	32.0	32.0	32.0	21.0	32.0
2	29.808317267970256	32.0	32.0	32.0	21.0	32.0
3	30.667860093638115	32.0	32.0	32.0	32.0	32.0
4	31.11952630129441	32.0	32.0	32.0	32.0	32.0
5	30.811071330212062	32.0	32.0	32.0	32.0	32.0
6	34.40870283668411	36.0	36.0	36.0	32.0	36.0
7	34.502340952905534	36.0	36.0	36.0	32.0	36.0
8	34.25144588267695	36.0	36.0	36.0	32.0	36.0
9	34.62351969154503	36.0	36.0	36.0	32.0	36.0
10	34.245937758193335	36.0	36.0	36.0	32.0	36.0
11	34.593225006885156	36.0	36.0	36.0	32.0	36.0
12	34.58799228862572	36.0	36.0	36.0	32.0	36.0
13	34.61388047369871	36.0	36.0	36.0	32.0	36.0
14	34.608923161663455	36.0	36.0	36.0	32.0	36.0
15	34.35031671715781	36.0	36.0	36.0	32.0	36.0
16	34.49628201597356	36.0	36.0	36.0	32.0	36.0
17	34.41393555494354	36.0	36.0	36.0	32.0	36.0
18	34.472321674469846	36.0	36.0	36.0	32.0	36.0
19	34.4288074910493	36.0	36.0	36.0	32.0	36.0
20	34.59487744423024	36.0	36.0	36.0	32.0	36.0
21	34.3974111814927	36.0	36.0	36.0	32.0	36.0
22	34.3888735885431	36.0	36.0	36.0	32.0	36.0
23	34.44092536491325	36.0	36.0	36.0	32.0	36.0
24	34.32966125034426	36.0	36.0	36.0	32.0	36.0
25	34.3291104378959	36.0	36.0	36.0	32.0	36.0
26	34.17653538969981	36.0	36.0	36.0	32.0	36.0
27	34.242357477278986	36.0	36.0	36.0	32.0	36.0
28	34.22335444781052	36.0	36.0	36.0	32.0	36.0
29	33.9586890663729	36.0	36.0	36.0	32.0	36.0
30	34.20132194987607	36.0	36.0	36.0	32.0	36.0
31	33.93335169374828	36.0	36.0	36.0	32.0	36.0
32	33.8190581107133	36.0	36.0	36.0	32.0	36.0
33	33.90581107133021	36.0	36.0	36.0	32.0	36.0
34	33.81713026714404	36.0	36.0	36.0	32.0	36.0
35	33.71467915174883	36.0	36.0	36.0	32.0	36.0
36	33.6196640044065	36.0	36.0	36.0	27.0	36.0
37	33.54144863673919	36.0	36.0	36.0	21.0	36.0
38	33.52382263839163	36.0	36.0	36.0	21.0	36.0
39	33.45910217570917	36.0	36.0	36.0	21.0	36.0
40	33.54089782429083	36.0	36.0	36.0	21.0	36.0
41	33.5370421371523	36.0	36.0	36.0	21.0	36.0
42	33.415863398512805	36.0	36.0	36.0	21.0	36.0
43	33.26852106857615	36.0	36.0	36.0	21.0	36.0
44	33.244836133296616	36.0	36.0	36.0	21.0	36.0
45	33.18149270173506	36.0	36.0	36.0	21.0	36.0
46	33.083723492150924	36.0	36.0	36.0	14.0	36.0
47	33.4687413935555	36.0	36.0	36.0	21.0	36.0
48	32.90278160286422	36.0	36.0	36.0	14.0	36.0
49	33.14568989259157	36.0	36.0	36.0	14.0	36.0
50	32.32222528229138	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	3.0
25	20.0
26	17.0
27	41.0
28	62.0
29	116.0
30	177.0
31	257.0
32	344.0
33	543.0
34	871.0
35	1179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.231404958677686	20.05509641873278	13.168044077134985	24.545454545454547
2	16.965023409529056	20.903332415312587	37.95097769209584	24.180666483062517
3	21.151198017075185	24.869182043514186	29.08289727347838	24.89672266593225
4	13.908014321123657	17.047645276783253	37.17984026438997	31.86450013770311
5	16.634535940512254	36.353621591847975	32.19498760671991	14.816854860919856
6	34.53594051225558	37.37262462131644	14.651611126411456	13.439823740016523
7	28.697328559625447	31.616634535940513	19.498760671991185	20.18727623244285
8	26.521619388598182	36.51886532635638	18.700082621867253	18.25943266317819
9	26.163591297163315	13.219498760671991	17.76370145965299	42.853208482511704
10	14.761773616083723	28.118975488846047	31.534012668686312	25.585238226383915
11	38.77719636463784	19.774166896171856	22.583310382814652	18.865326356375654
12	25.392453869457448	23.519691545028916	29.68879096667585	21.399063618837786
13	30.48746901679978	19.939410630680253	23.684935279537317	25.88818507298265
14	22.225282291379784	19.526301294409254	25.640319471220053	32.60809694299091
15	23.106582208757917	30.515009639217844	21.86725419994492	24.51115395207932
16	24.373450839988983	24.759019553841917	26.30129440925365	24.56623519691545
17	22.362985403470116	27.43045992839438	26.659322500688514	23.547232167446985
18	22.99641971908565	24.979344533186453	29.275681630404847	22.748554117323053
19	25.172128890112916	25.419994491875514	24.759019553841917	24.64885706416965
20	26.21867254199945	25.034425778022584	25.97080694023685	22.77609473974112
21	24.64885706416965	25.943266317818782	24.15312586064445	25.254750757367116
22	24.951803910768383	27.29275681630405	25.998347562654917	21.757091710272654
23	22.913797851831454	26.76948499036078	25.419994491875514	24.89672266593225
24	24.924263288350318	25.860644450564585	26.82456623519692	22.390526025888185
25	23.244285320848252	26.934728724869185	26.328835031671716	23.49215092261085
26	23.636363636363637	26.391184573002757	26.611570247933887	23.360881542699723
27	22.58953168044077	27.713498622589533	26.30853994490358	23.388429752066113
28	23.471074380165287	25.3168044077135	26.942148760330582	24.269972451790633
29	22.83746556473829	26.391184573002757	25.564738292011018	25.206611570247933
30	24.380165289256198	26.005509641873275	26.225895316804408	23.388429752066113
31	25.041322314049587	25.730027548209367	24.931129476584022	24.297520661157023
32	23.608815426997246	26.639118457300277	24.931129476584022	24.820936639118457
33	22.865013774104685	27.107438016528924	26.749311294765842	23.27823691460055
34	24.187327823691458	26.060606060606062	26.30853994490358	23.443526170798897
35	23.443526170798897	28.29201101928375	25.206611570247933	23.05785123966942
36	22.61707988980716	27.079889807162534	26.72176308539945	23.581267217630856
37	24.40771349862259	25.3168044077135	26.225895316804408	24.049586776859506
38	24.297520661157023	26.170798898071624	25.399449035812673	24.132231404958677
39	23.939393939393938	26.446280991735538	25.785123966942148	23.829201101928373
40	26.328835031671716	25.943266317818782	25.722941338474246	22.004957312035252
41	24.538694574497384	24.814100798678048	27.210134949049845	23.43706967777472
42	25.123966942148762	27.685950413223143	24.793388429752067	22.396694214876035
43	21.481685486091987	27.705866152575048	26.05342880749105	24.759019553841917
44	22.19774166896172	27.29275681630405	25.089507022858715	25.419994491875514
45	23.02396034150372	28.118975488846047	25.006885155604518	23.850179014045718
46	23.65739465711925	26.05342880749105	25.447535114293586	24.841641421096117
47	24.428532084825115	25.22721013494905	27.017350592123385	23.32690718810245
48	24.042963370972185	26.604241255852383	25.06196640044065	24.290828972734786
49	24.621316441751585	26.549160011016248	25.53015698154778	23.299366565684384
50	24.23574772789865	26.521619388598182	24.51115395207932	24.73147893142385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	2.5
6	4.0
7	2.5
8	1.0
9	3.0
10	5.0
11	5.0
12	5.0
13	6.0
14	7.0
15	9.0
16	11.0
17	9.0
18	7.0
19	12.5
20	18.0
21	17.0
22	16.0
23	16.5
24	17.0
25	23.0
26	29.0
27	38.5
28	48.0
29	74.0
30	100.0
31	93.5
32	87.0
33	115.0
34	143.0
35	166.5
36	190.0
37	201.0
38	212.0
39	202.5
40	193.0
41	198.0
42	203.0
43	192.0
44	181.0
45	180.0
46	179.0
47	196.0
48	213.0
49	204.0
50	195.0
51	200.5
52	206.0
53	208.5
54	211.0
55	207.5
56	204.0
57	196.5
58	189.0
59	191.0
60	193.0
61	172.5
62	152.0
63	133.0
64	114.0
65	99.0
66	84.0
67	77.0
68	70.0
69	63.5
70	57.0
71	42.5
72	28.0
73	26.5
74	25.0
75	16.5
76	8.0
77	6.5
78	5.0
79	5.0
80	5.0
81	6.0
82	7.0
83	5.5
84	4.0
85	3.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02754062241806665
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.02754062241806665
27	0.02754062241806665
28	0.02754062241806665
29	0.02754062241806665
30	0.02754062241806665
31	0.02754062241806665
32	0.02754062241806665
33	0.02754062241806665
34	0.02754062241806665
35	0.02754062241806665
36	0.02754062241806665
37	0.02754062241806665
38	0.02754062241806665
39	0.02754062241806665
40	0.0
41	0.0
42	0.02754062241806665
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3631.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.08923161663454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.87589567211235	93.08730377306527
2	2.5508741759816567	4.902230790415863
3	0.3725995987388937	1.0740842743045993
4	0.14330753797649756	0.5508124483613329
5	0.0	0.0
6	0.0	0.0
7	0.05732301519059903	0.38556871385293306
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	7	0.19278435692646653	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	7	0.19278435692646653	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG	4	0.1101624896722666	No Hit
AAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGCTTGGGTACG	4	0.1101624896722666	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	4	0.1101624896722666	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	4	0.1101624896722666	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	4	0.1101624896722666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.027540622418066648	0.0
33	0.0	0.0	0.0	0.027540622418066648	0.0
34	0.0	0.0	0.0	0.027540622418066648	0.0
35	0.0	0.0	0.0	0.055081244836133296	0.0
36	0.0	0.0	0.0	0.08262186725419994	0.0
37	0.0	0.0	0.0	0.11016248967226659	0.0
38	0.0	0.0	0.0	0.11016248967226659	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE