FastQCFastQC Report
Thu 2 Feb 2017
SRR4064120_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064120_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095854
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT16080.14673487526623072No Hit
GTCCTACAGTGGACATTTCTAAATT15840.1445448025010631No Hit
CTGTAGGACGTGGAATATGGCAAGA15280.13943463271567197No Hit
CTTTAGGACGTGAAATATGGCGAGG14710.13423320989839888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT250.00603423218.9982191
GGTATCA4850.015.4727751
GATAATC456.7625334E-414.7770687
CTTGCGC456.7812914E-414.771673
ACGGACC400.00527626814.2506178
ATTAGAC551.9662028E-413.8124713
GTATCAA15400.012.8299671
GTCTAGA1051.9936124E-812.6654791
GACGTGG3900.011.6917457
GTGCTAA752.0745526E-411.39893151
GTGTATA2000.011.3989311
ATTTAGA2950.011.270131
GCATTAG855.3243763E-511.1754241
GTAGGAC8200.011.1173543
CGAAATC600.005869891511.08431913
CAAGACG600.005889880511.0792584
TCTAGAC951.365308E-510.9954543
TTCTATA1303.28273E-810.9565092
TAGGACC3400.010.8930534
TGTAGGA9000.010.8672322
Produced by FastQC (version 0.11.5)