##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064120_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1095854
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38464977998894	32.0	32.0	32.0	32.0	32.0
2	31.127872873576223	32.0	32.0	32.0	32.0	32.0
3	31.120805326256963	32.0	32.0	32.0	32.0	32.0
4	31.139894547996356	32.0	32.0	32.0	32.0	32.0
5	31.139284977743387	32.0	32.0	32.0	32.0	32.0
6	34.79630863235431	36.0	36.0	36.0	36.0	36.0
7	34.74562761097737	36.0	36.0	36.0	36.0	36.0
8	34.725668747844146	36.0	36.0	36.0	32.0	36.0
9	34.79137549345077	36.0	36.0	36.0	32.0	36.0
10	34.61936079076227	36.0	36.0	36.0	32.0	36.0
11	34.76658751987035	36.0	36.0	36.0	36.0	36.0
12	34.650789247472744	36.0	36.0	36.0	32.0	36.0
13	34.676205954442835	36.0	36.0	36.0	32.0	36.0
14	34.62408131010153	36.0	36.0	36.0	32.0	36.0
15	34.60798883792914	36.0	36.0	36.0	32.0	36.0
16	34.59927964856632	36.0	36.0	36.0	32.0	36.0
17	34.56639479346701	36.0	36.0	36.0	32.0	36.0
18	34.55349891500145	36.0	36.0	36.0	32.0	36.0
19	34.54922553551842	36.0	36.0	36.0	32.0	36.0
20	34.52174103484588	36.0	36.0	36.0	32.0	36.0
21	34.51003053326447	36.0	36.0	36.0	32.0	36.0
22	34.49331115276305	36.0	36.0	36.0	32.0	36.0
23	34.45304575244513	36.0	36.0	36.0	32.0	36.0
24	34.455713991097355	36.0	36.0	36.0	32.0	36.0
25	34.076647071598956	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	13.0
5	22.0
6	47.0
7	19.0
8	80.0
9	67.0
10	117.0
11	22.0
12	93.0
13	56.0
14	88.0
15	135.0
16	236.0
17	320.0
18	392.0
19	470.0
20	695.0
21	1015.0
22	1486.0
23	2417.0
24	3653.0
25	5859.0
26	8514.0
27	11920.0
28	16888.0
29	23261.0
30	31412.0
31	43264.0
32	61043.0
33	88871.0
34	208188.0
35	585186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15987420752108	18.74280550091058	12.126833142392886	25.970487149175458
2	14.779345602152244	21.18287694860128	40.458720954638956	23.579056494607524
3	18.26073704728462	24.92197709137897	29.574971345972738	27.24231451536367
4	11.821548379089691	17.57339816244511	38.19944077590518	32.40561268256002
5	12.82251124129239	38.17813946229798	35.45978642685243	13.539562869557203
6	30.673363366871598	38.04231954321763	18.8618956176346	12.422421472276172
7	28.375976786237505	31.887158769466122	21.960975102162646	17.775889342133727
8	25.642025157910208	36.02899759996567	20.695434822498793	17.633542419625325
9	26.970270524440693	15.395944096424262	20.02441467327342	37.60937070586162
10	14.411007887561613	29.71422883973037	34.28039468854267	21.59436858416534
11	33.804585232585325	23.06632407082749	24.46355262388967	18.665538072697522
12	23.797582791889067	25.622679932241464	30.818535885997633	19.761201389871836
13	28.793471233467145	22.335840276433398	25.753178728362546	23.117509761736912
14	21.48073468724288	22.184720163718403	27.535064576900986	28.799480572137735
15	22.749109451005424	30.871208489367746	23.633586001784295	22.74609605784254
16	22.05983610000566	28.62478517119641	27.287413086795404	22.027965642002524
17	21.61482470406569	28.72437085137217	27.377542140333546	22.2832623042286
18	21.555306336040854	27.70010977429601	29.361153780820366	21.383430108842767
19	23.54667728454952	26.971309798649873	27.53897688418715	21.94303603261346
20	24.473186943858664	26.998912262211476	27.472639878421784	21.05526091550807
21	24.103463769598317	26.818643423878896	26.182291742770726	22.895601063752064
22	23.911540551524478	27.95404419319339	26.647975049430805	21.486440205851327
23	22.431949117557874	27.994019632556288	27.237523243792218	22.33650800609362
24	23.01272655040618	27.319844194510278	27.78415551324027	21.883273741843272
25	22.622404956473773	27.698881797890724	27.5014338266184	22.177279419017108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	24.0
2	110.5
3	197.0
4	197.0
5	197.0
6	397.0
7	597.0
8	597.0
9	597.0
10	1048.0
11	1499.0
12	1499.0
13	1499.0
14	2591.0
15	3683.0
16	3683.0
17	3683.0
18	6598.0
19	9513.0
20	9513.0
21	9513.0
22	15965.0
23	22417.0
24	22417.0
25	22417.0
26	33023.0
27	43629.0
28	43629.0
29	43629.0
30	58312.5
31	72996.0
32	72996.0
33	72996.0
34	87497.5
35	101999.0
36	101999.0
37	101999.0
38	115528.5
39	129058.0
40	129058.0
41	129058.0
42	139396.0
43	149734.0
44	149734.0
45	149734.0
46	155744.5
47	161755.0
48	161755.0
49	161755.0
50	154683.5
51	147612.0
52	147612.0
53	147612.0
54	128653.0
55	109694.0
56	109694.0
57	109694.0
58	91051.0
59	72408.0
60	72408.0
61	72408.0
62	56038.0
63	39668.0
64	39668.0
65	39668.0
66	28678.0
67	17688.0
68	17688.0
69	17688.0
70	12298.5
71	6909.0
72	6909.0
73	6909.0
74	4622.0
75	2335.0
76	2335.0
77	2335.0
78	1700.5
79	1066.0
80	1066.0
81	1066.0
82	748.0
83	430.0
84	430.0
85	430.0
86	335.5
87	241.0
88	241.0
89	241.0
90	193.5
91	146.0
92	146.0
93	146.0
94	120.5
95	95.0
96	95.0
97	95.0
98	278.0
99	461.0
100	461.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036409959720911725
2	0.006205206167974931
3	0.002007566701403654
4	0.004562651594099214
5	0.01067660473019216
6	0.017338076057577013
7	0.02710215046894933
8	0.04133762344253888
9	0.05575560247989239
10	0.0649721586999728
11	0.06259957987104121
12	0.07199864215488559
13	0.06953481029407202
14	0.07327618460123338
15	0.06780100268831432
16	0.07263741337805948
17	0.069626063325954
18	0.08030266805614616
19	0.0772000649721587
20	0.08404404236330752
21	0.07866011348227045
22	0.08112394534308402
23	0.08559534390530125
24	0.08203647566190386
25	0.08048517411991013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1095854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.620083980090534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.881344515951646	32.9756036867452
2	18.148705316762992	18.736753851624425
3	7.9429629968890385	12.300492508504913
4	4.101335447958022	8.468451210164615
5	2.1415264153185563	5.527288670216306
6	1.230848226219677	3.812189328252312
7	0.7766387362738983	2.806310975205501
8	0.49488377745691536	2.0436753722168324
9	0.3120727070367011	1.4498297410615832
>10	0.9073961462044006	6.94175700489944
>50	0.036063362525399024	1.285599832609593
>100	0.02354660341736361	2.4158529138211917
>500	0.001962215189326384	0.6661179843904628
>1k	7.135327961186851E-4	0.5700769202877184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	1608	0.14673487526623072	No Hit
GTCCTACAGTGGACATTTCTAAATT	1584	0.1445448025010631	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1528	0.13943463271567197	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1471	0.13423320989839888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.125303188198428E-5	0.0
8	0.0	0.0	0.0	9.125303188198428E-5	0.0
9	0.0	0.0	0.0	1.8250606376396856E-4	0.0
10	0.0	0.0	0.0	1.8250606376396856E-4	0.0
11	0.0	0.0	0.0	4.562651594099214E-4	0.0
12	0.0	0.0	0.0	4.562651594099214E-4	0.0
13	0.0	0.0	0.0	8.212772869378585E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	25	0.006034232	18.998219	1
GGTATCA	485	0.0	15.472775	1
GATAATC	45	6.7625334E-4	14.777068	7
CTTGCGC	45	6.7812914E-4	14.77167	3
ACGGACC	40	0.005276268	14.250617	8
ATTAGAC	55	1.9662028E-4	13.812471	3
GTATCAA	1540	0.0	12.829967	1
GTCTAGA	105	1.9936124E-8	12.665479	1
GACGTGG	390	0.0	11.691745	7
GTGCTAA	75	2.0745526E-4	11.3989315	1
GTGTATA	200	0.0	11.398931	1
ATTTAGA	295	0.0	11.27013	1
GCATTAG	85	5.3243763E-5	11.175424	1
GTAGGAC	820	0.0	11.117354	3
CGAAATC	60	0.0058698915	11.084319	13
CAAGACG	60	0.0058898805	11.079258	4
TCTAGAC	95	1.365308E-5	10.995454	3
TTCTATA	130	3.28273E-8	10.956509	2
TAGGACC	340	0.0	10.893053	4
TGTAGGA	900	0.0	10.867232	2
>>END_MODULE