FastQCFastQC Report
Thu 2 Feb 2017
SRR4064120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095854
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.032.588641
AAGTCCG353.2199576E-431.4247575
CTCGATA300.005745625629.3324512
GTATCAA17750.026.8925131
CGTCGTA508.3424355E-526.39920610
CCGTCGT551.5933171E-424.0003749
GTCGTAG602.873124E-421.99933811
ACCGTCG602.874898E-421.9973328
CGCGGTT654.9327576E-420.30708310
TACCGTC654.935794E-420.305237
TAGGACG10800.020.164224
GCACGAC550.00448768219.9966616
TTAGGAC7600.019.971263
GACGTGA6500.019.9668067
TAGGACC4750.019.9133744
CGAACTC1001.1240554E-619.79850220
AGGACGT11000.019.597625
ATGTCGA808.999123E-519.24678617
GACCGCG708.129289E-418.8548557
GTCCTAC9000.018.8199391
Produced by FastQC (version 0.11.5)