##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064120_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1095854
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.350971936042576	32.0	32.0	32.0	32.0	32.0
2	31.497931293767234	32.0	32.0	32.0	32.0	32.0
3	31.53842847678614	32.0	32.0	32.0	32.0	32.0
4	31.59914185648818	32.0	32.0	32.0	32.0	32.0
5	31.592296966566714	32.0	32.0	32.0	32.0	32.0
6	35.185021909852956	36.0	36.0	36.0	36.0	36.0
7	35.185138713733764	36.0	36.0	36.0	36.0	36.0
8	35.14000678922557	36.0	36.0	36.0	36.0	36.0
9	35.21899632615294	36.0	36.0	36.0	36.0	36.0
10	35.11422963277955	36.0	36.0	36.0	36.0	36.0
11	35.219141418473626	36.0	36.0	36.0	36.0	36.0
12	35.15484818233086	36.0	36.0	36.0	36.0	36.0
13	35.17715863609568	36.0	36.0	36.0	36.0	36.0
14	35.13396218839371	36.0	36.0	36.0	36.0	36.0
15	35.12404845901005	36.0	36.0	36.0	36.0	36.0
16	35.141381972416035	36.0	36.0	36.0	36.0	36.0
17	35.13003648296215	36.0	36.0	36.0	36.0	36.0
18	35.107821844880796	36.0	36.0	36.0	36.0	36.0
19	35.095541011850116	36.0	36.0	36.0	36.0	36.0
20	35.091964805530665	36.0	36.0	36.0	36.0	36.0
21	35.09295855104786	36.0	36.0	36.0	36.0	36.0
22	35.06596499168685	36.0	36.0	36.0	36.0	36.0
23	35.0265035305798	36.0	36.0	36.0	36.0	36.0
24	35.02641045248728	36.0	36.0	36.0	36.0	36.0
25	34.99193323198163	36.0	36.0	36.0	36.0	36.0
26	34.94540513608565	36.0	36.0	36.0	36.0	36.0
27	34.923294526460644	36.0	36.0	36.0	36.0	36.0
28	34.90545729631867	36.0	36.0	36.0	36.0	36.0
29	34.888775329560325	36.0	36.0	36.0	36.0	36.0
30	34.872440124323134	36.0	36.0	36.0	36.0	36.0
31	34.86056627981465	36.0	36.0	36.0	36.0	36.0
32	34.83678117705461	36.0	36.0	36.0	32.0	36.0
33	34.83957078223924	36.0	36.0	36.0	32.0	36.0
34	34.83469878286706	36.0	36.0	36.0	36.0	36.0
35	34.80192434393633	36.0	36.0	36.0	32.0	36.0
36	34.79071025884835	36.0	36.0	36.0	32.0	36.0
37	34.76797091583368	36.0	36.0	36.0	32.0	36.0
38	34.750273302830486	36.0	36.0	36.0	32.0	36.0
39	34.7265876658752	36.0	36.0	36.0	32.0	36.0
40	34.73555145119697	36.0	36.0	36.0	32.0	36.0
41	34.70939103201704	36.0	36.0	36.0	32.0	36.0
42	34.68158805826324	36.0	36.0	36.0	32.0	36.0
43	34.67817701993149	36.0	36.0	36.0	32.0	36.0
44	34.64142121121974	36.0	36.0	36.0	32.0	36.0
45	34.62289775827802	36.0	36.0	36.0	32.0	36.0
46	34.602403239847646	36.0	36.0	36.0	32.0	36.0
47	34.59906429141108	36.0	36.0	36.0	32.0	36.0
48	34.56829103146952	36.0	36.0	36.0	32.0	36.0
49	34.550633569800354	36.0	36.0	36.0	32.0	36.0
50	34.00219281035612	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	2.0
19	3.0
20	13.0
21	26.0
22	113.0
23	303.0
24	823.0
25	1862.0
26	3602.0
27	6748.0
28	11160.0
29	17068.0
30	24515.0
31	34660.0
32	49030.0
33	75473.0
34	158116.0
35	712334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3018758065311	19.66986754181185	12.627060019603153	26.401196632053896
2	14.176223184033567	21.938423998196832	39.82793249799927	24.057420319770333
3	17.545316503748694	25.482183660507662	29.79847570100963	27.174024134734015
4	11.463753383206157	18.337935527907913	38.167493114958745	32.030817973927185
5	12.54272923217874	38.6205644182528	35.27458949823608	13.562116851332384
6	30.85432078937595	38.261336146362694	18.60641892662611	12.27792413763525
7	28.313012785473962	32.05064912903464	21.94810800353697	17.688230081954423
8	25.584247536624403	36.32564191945277	20.53950617509267	17.550604368830154
9	26.886315520513943	15.388286611184116	19.929277996787853	37.79611987151409
10	14.34458617209415	29.88733777314613	34.24818541359064	21.519890641169074
11	33.9165015745725	23.173363580699235	24.3886725683098	18.52146227641846
12	23.69145075379773	25.772025576469794	30.78338204737688	19.753141622355592
13	28.87394761672717	22.338996537834696	25.750424785964448	23.036631059473685
14	21.601659349674364	22.243534933125034	27.430188958170408	28.724616759030198
15	22.73383500913493	31.06687935871158	23.521026762329416	22.678258869824074
16	22.138227956219968	28.766555762600817	27.11458403674982	21.980632244429398
17	21.686830544944854	28.788141486000875	27.255455562511067	22.269572406543205
18	21.56968849694483	27.895109540739227	29.307531701476847	21.227670260839094
19	23.596756502234786	27.02458539184964	27.495451036360684	21.883207069554885
20	24.504605130479458	27.035350866866874	27.43194052432693	21.02810347832673
21	24.18789364276628	26.69233310276734	26.344020280073803	22.775752974392574
22	23.94789432860337	28.035680065702422	26.51996167358671	21.496463932107496
23	22.528703955497235	28.054318049499837	27.103542298981058	22.313435696021873
24	23.07358880718641	27.420002445599106	27.62377195335841	21.882636793856072
25	22.737389637944013	27.786097232678575	27.411311112632035	22.065202016745374
26	21.829386931151358	28.29682614718836	27.60434253298606	22.269444388674227
27	22.553617498546686	27.864398674540574	27.125284388800214	22.456699438112526
28	21.794944332907466	28.210987406461037	27.05219930644278	22.94186895418872
29	22.684406968361305	27.62258055684836	26.747976382767085	22.94503609202325
30	22.21175792054857	27.639353926713994	27.40162567793617	22.74726247480126
31	23.133771935828605	27.130384311062805	27.02944915505593	22.70639459805266
32	22.186540365670588	27.863332101351073	27.315784430481976	22.63434310249636
33	21.9219411053963	27.99683880458629	27.899192179509175	22.182027910508236
34	22.208047015449758	27.94270904627627	27.954024876575318	21.89521906169865
35	22.46293820524637	27.845282691719785	27.65145258508585	22.040326517947992
36	21.96845248525203	27.61073754453595	28.0426815022487	22.37812846796332
37	22.23217944166768	27.103693286816288	27.099769209499186	23.56435806201685
38	21.97784346597624	27.830096126028863	27.530301191239964	22.66175921675493
39	22.971399368486374	26.724981291865156	27.464910839767104	22.838708499881363
40	23.066835674530278	26.608700254698135	28.081131075088955	22.243332995682632
41	22.86987985891522	26.378109044118652	28.27107260025826	22.48093849670787
42	22.188295629796592	27.36053949973992	28.19780440396777	22.253360466495717
43	21.74900189355052	27.15077681199097	28.366664385280497	22.733556909178017
44	21.495167620731912	27.09225915898076	28.296909120608266	23.11566409967906
45	21.656683348116676	27.091934135649026	28.089992225057216	23.161390291177085
46	21.640131442666306	26.453429746522982	28.47890976685444	23.427529043956273
47	22.117703744870276	26.29886870003166	28.51447182427025	23.06895573082781
48	21.12112587114572	26.81271770087797	28.60424136291586	23.461915065060452
49	21.88486682411	26.397644190231457	28.345001660820333	23.372487324838207
50	21.21306694262087	27.379055586886338	28.163572219880017	23.244305250612776
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	6.5
4	12.0
5	17.5
6	23.0
7	29.5
8	36.0
9	66.0
10	96.0
11	142.5
12	189.0
13	367.0
14	545.0
15	726.0
16	907.0
17	1080.0
18	1253.0
19	1543.5
20	1834.0
21	2414.0
22	2994.0
23	4117.5
24	5241.0
25	7051.0
26	8861.0
27	11783.0
28	14705.0
29	18879.5
30	23054.0
31	28091.0
32	33128.0
33	40273.0
34	47418.0
35	55041.0
36	62664.0
37	67310.5
38	71957.0
39	74934.5
40	77912.0
41	80086.0
42	82260.0
43	84598.0
44	86936.0
45	88677.5
46	90419.0
47	90661.5
48	90904.0
49	89725.5
50	88547.0
51	83972.0
52	79397.0
53	73222.0
54	67047.0
55	60173.5
56	53300.0
57	46741.5
58	40183.0
59	33630.5
60	27078.0
61	21640.5
62	16203.0
63	12941.5
64	9680.0
65	7387.5
66	5095.0
67	3869.0
68	2643.0
69	1989.5
70	1336.0
71	1017.5
72	699.0
73	557.5
74	416.0
75	318.0
76	220.0
77	223.0
78	226.0
79	175.0
80	124.0
81	107.5
82	91.0
83	79.0
84	67.0
85	59.0
86	51.0
87	46.5
88	42.0
89	35.0
90	28.0
91	21.0
92	14.0
93	12.5
94	11.0
95	6.5
96	2.0
97	3.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01022033957078224
2	4.562651594099214E-4
3	5.475181912919057E-4
4	0.0
5	0.0
6	9.125303188198428E-5
7	9.125303188198428E-5
8	0.0
9	0.00127754244634778
10	0.0010950363825838114
11	9.125303188198428E-5
12	2.7375909564595285E-4
13	7.300242550558742E-4
14	2.7375909564595285E-4
15	0.005475181912919057
16	0.0010950363825838114
17	0.0
18	5.475181912919057E-4
19	0.0
20	0.001003783350701827
21	0.0
22	3.650121275279371E-4
23	0.0010950363825838114
24	7.300242550558742E-4
25	0.0026463379245775438
26	0.008030266805614616
27	0.007574001646204695
28	0.004927663721627151
29	0.004015133402807308
30	0.005931447072328978
31	0.009216556220080413
32	0.005383928881037073
33	0.0060227001042109625
34	0.004015133402807308
35	0.004471398562217229
36	0.01003783350701827
37	0.005018916753509135
38	0.00958156834760835
39	0.006752724359266837
40	0.004288892498453261
41	0.005383928881037073
42	0.0021900727651676228
43	0.0026463379245775438
44	0.0015513015419937328
45	0.0023725788289315915
46	0.0013687954782297643
47	0.0031938561158694496
48	0.0024638318608135755
49	9.125303188198428E-4
50	0.0018250606376396855
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1095854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.67699720584258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.89962625369932	40.42823177171913
2	16.358225355902107	19.197030870016224
3	6.758811092373245	11.89760218746008
4	3.3290550495242335	7.813558153561184
5	1.7832861185485276	5.231893729764491
6	1.0120123130460743	3.562910619892968
7	0.6157263611408279	2.529028178055679
8	0.38450500539655647	1.8049279301828987
9	0.22981211550871095	1.2136216373616064
>10	0.6094941426406558	5.038949288960771
>50	0.013492535450108117	0.5329979855749089
>100	0.005326956978616642	0.49202147854476364
>500	6.2669979104105E-4	0.25722616890528566
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.125303188198428E-5	0.0
4	0.0	0.0	0.0	9.125303188198428E-5	0.0
5	0.0	0.0	0.0	9.125303188198428E-5	0.0
6	0.0	0.0	0.0	9.125303188198428E-5	0.0
7	0.0	0.0	0.0	1.8250606376396856E-4	0.0
8	0.0	0.0	0.0	1.8250606376396856E-4	0.0
9	0.0	0.0	0.0	5.475181912919057E-4	0.0
10	0.0	0.0	0.0	5.475181912919057E-4	0.0
11	0.0	0.0	0.0	8.212772869378585E-4	0.0
12	0.0	0.0	0.0	8.212772869378585E-4	0.0
13	0.0	0.0	0.0	8.212772869378585E-4	0.0
14	0.0	0.0	0.0	8.212772869378585E-4	0.0
15	0.0	0.0	0.0	8.212772869378585E-4	0.0
16	0.0	0.0	0.0	0.001003783350701827	0.0
17	0.0	0.0	0.0	0.001003783350701827	0.0
18	0.0	0.0	0.0	0.0014600485101117484	0.0
19	0.0	0.0	0.0	0.0015513015419937328	0.0
20	0.0	0.0	0.0	0.0015513015419937328	0.0
21	0.0	0.0	0.0	0.0018250606376396855	0.0
22	0.0	0.0	0.0	0.002281325797049607	0.0
23	0.0	0.0	0.0	0.0024638318608135755	0.0
24	0.0	0.0	0.0	0.0028288439883415125	0.0
25	0.0	0.0	0.0	0.0030113500521054813	0.0
26	0.0	0.0	0.0	0.0034676152115154027	0.0
27	0.0	0.0	0.0	0.004562651594099214	0.0
28	0.0	0.0	0.0	0.010767857762074146	0.0
29	0.0	0.0	0.0	0.022995764034260038	0.0
30	0.0	0.0	0.0	0.05210548120461302	0.0
31	0.0	0.0	0.0	0.13961713877943593	0.0
32	0.0	0.0	0.0	0.23689287076563117	0.0
33	0.0	0.0	0.0	0.34046506195168336	0.0
34	0.0	0.0	0.0	0.45115498962453027	0.0
35	0.0	0.0	0.0	0.5811905600563578	0.0
36	0.0	0.0	0.0	0.7639703829159724	0.0
37	0.0	0.0	0.0	1.0429309013791983	0.0
38	0.0	0.0	0.0	1.4424366749585256	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	675	0.0	32.58864	1
AAGTCCG	35	3.2199576E-4	31.424757	5
CTCGATA	30	0.0057456256	29.33245	12
GTATCAA	1775	0.0	26.892513	1
CGTCGTA	50	8.3424355E-5	26.399206	10
CCGTCGT	55	1.5933171E-4	24.000374	9
GTCGTAG	60	2.873124E-4	21.999338	11
ACCGTCG	60	2.874898E-4	21.997332	8
CGCGGTT	65	4.9327576E-4	20.307083	10
TACCGTC	65	4.935794E-4	20.30523	7
TAGGACG	1080	0.0	20.16422	4
GCACGAC	55	0.004487682	19.99666	16
TTAGGAC	760	0.0	19.97126	3
GACGTGA	650	0.0	19.966806	7
TAGGACC	475	0.0	19.913374	4
CGAACTC	100	1.1240554E-6	19.798502	20
AGGACGT	1100	0.0	19.59762	5
ATGTCGA	80	8.999123E-5	19.246786	17
GACCGCG	70	8.129289E-4	18.854855	7
GTCCTAC	900	0.0	18.819939	1
>>END_MODULE