FastQCFastQC Report
Thu 2 Feb 2017
SRR4064119_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064119_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869205
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35540.19013430843593934No Hit
TATCAACGCAGAGTACTTTTTTTTT23460.1255079030924912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG400.005283137314.2485745
CGTTATT2050.012.9701452
GTTATTC2300.012.8006693
ACAACGC751.4780406E-512.6674348
GCACTAT751.4796689E-512.6660781
GCGTTAT2300.012.3907291
GTATCAA24700.012.1917411
TATTCCC2750.012.08975
CGAACGA1603.6379788E-1211.87635516
ACTCTAA2500.011.78071410
GTATTAG2200.011.6585481
AATCGCT1552.5465852E-1111.64649115
ACCGTCG3350.011.6275698
CCGTCGT3300.011.5158499
CGTCGTA3300.011.51584910
CGATAAC1657.2759576E-1211.51584910
ATAACGA1657.2759576E-1211.51554112
CGGACCA3550.011.507749
AATTCCG1900.011.498855
TGAACTG2000.011.398865
Produced by FastQC (version 0.11.5)