##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064119_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1869205
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.247174600966723	32.0	32.0	32.0	32.0	32.0
2	30.776636056505307	32.0	32.0	32.0	32.0	32.0
3	30.793556084003626	32.0	32.0	32.0	32.0	32.0
4	30.83175307149296	32.0	32.0	32.0	32.0	32.0
5	30.70925125922518	32.0	32.0	32.0	32.0	32.0
6	34.39198482777437	36.0	36.0	36.0	32.0	36.0
7	34.322896097538795	36.0	36.0	36.0	32.0	36.0
8	34.27990990822301	36.0	36.0	36.0	32.0	36.0
9	34.45022349073537	36.0	36.0	36.0	32.0	36.0
10	34.08355851819356	36.0	36.0	36.0	32.0	36.0
11	34.422688790154105	36.0	36.0	36.0	32.0	36.0
12	34.20199175585343	36.0	36.0	36.0	32.0	36.0
13	34.30971134787249	36.0	36.0	36.0	32.0	36.0
14	34.1826637527719	36.0	36.0	36.0	32.0	36.0
15	34.112224715855135	36.0	36.0	36.0	32.0	36.0
16	34.10837281090089	36.0	36.0	36.0	32.0	36.0
17	34.02776634986532	36.0	36.0	36.0	32.0	36.0
18	34.024574083634484	36.0	36.0	36.0	32.0	36.0
19	34.04011331020407	36.0	36.0	36.0	32.0	36.0
20	34.029325836384984	36.0	36.0	36.0	32.0	36.0
21	34.019642040332656	36.0	36.0	36.0	32.0	36.0
22	33.99052484879935	36.0	36.0	36.0	32.0	36.0
23	33.924841844527485	36.0	36.0	36.0	32.0	36.0
24	33.91244299046921	36.0	36.0	36.0	32.0	36.0
25	33.49252703689537	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	15.0
5	14.0
6	64.0
7	34.0
8	104.0
9	106.0
10	163.0
11	54.0
12	111.0
13	90.0
14	168.0
15	283.0
16	502.0
17	686.0
18	922.0
19	1282.0
20	2004.0
21	2994.0
22	4554.0
23	7403.0
24	10836.0
25	15735.0
26	22387.0
27	29252.0
28	39145.0
29	52964.0
30	69503.0
31	94223.0
32	134002.0
33	188522.0
34	397306.0
35	793767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.272984604054955	16.532912598964185	10.70887069108082	26.485232105900042
2	17.384056001523735	19.513004175289392	36.86447751100535	26.238462312181532
3	19.489495610620967	23.478001745158682	27.805267682839073	29.227234961381278
4	12.995274299798679	15.034820808061028	35.25662322960119	36.7132816625391
5	15.131313542046765	36.07090920569833	33.186323659551064	15.611453592703839
6	34.75871279245961	34.74110957016035	16.73493934646566	13.765238290914382
7	30.890356606038168	29.79249395204336	20.52848519354264	18.78866424837584
8	28.492253944740455	32.384173086926644	19.202392853549572	19.921180114783333
9	27.94168494498307	13.93637326088376	17.902312969736673	40.219628824396494
10	16.22945510701678	26.062098369255395	31.155331568678356	26.553114955049473
11	37.90001509440426	20.616708113831077	22.068661340447328	19.414615451317335
12	25.409592981862605	23.09142999688449	28.292294630726623	23.206682390526286
13	30.035421945118202	19.09193057362995	24.639905441553793	26.23274203969806
14	24.189184559281713	19.041314863629257	24.66022411182893	32.109276465260095
15	25.67634262173632	26.495019634544807	21.734284870052285	26.094352873666587
16	26.3066410996802	25.064318957791986	23.168903777982628	25.460136164545183
17	24.693136426753686	25.170574928242367	24.529279575867164	25.607009069136776
18	25.479064229299798	24.346399629940553	25.392278007161334	24.782258133598315
19	26.26615163463778	24.21110959421151	24.685988398394944	24.83675037275577
20	26.423285913223932	23.73479004140225	24.218521201452212	25.62340284392161
21	27.437374521508684	23.552158899269212	23.815027973338328	25.19543860588377
22	26.568533180283072	23.712225532124492	24.22550428419378	25.49373700339866
23	25.157130336126944	23.598650116693268	24.773025035377415	26.471194511802377
24	25.426699731880518	24.296079287984305	24.545513924224124	25.731707055911052
25	25.594900925692933	23.837851150289914	24.540713891818676	26.026534032198477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	111.0
1	111.0
2	305.5
3	500.0
4	500.0
5	500.0
6	1339.5
7	2179.0
8	2179.0
9	2179.0
10	2337.0
11	2495.0
12	2495.0
13	2495.0
14	2900.0
15	3305.0
16	3305.0
17	3305.0
18	6004.0
19	8703.0
20	8703.0
21	8703.0
22	14588.5
23	20474.0
24	20474.0
25	20474.0
26	31439.0
27	42404.0
28	42404.0
29	42404.0
30	54529.5
31	66655.0
32	66655.0
33	66655.0
34	84928.5
35	103202.0
36	103202.0
37	103202.0
38	122291.5
39	141381.0
40	141381.0
41	141381.0
42	165783.0
43	190185.0
44	190185.0
45	190185.0
46	215037.5
47	239890.0
48	239890.0
49	239890.0
50	250390.0
51	260890.0
52	260890.0
53	260890.0
54	247408.5
55	233927.0
56	233927.0
57	233927.0
58	216112.5
59	198298.0
60	198298.0
61	198298.0
62	175322.0
63	152346.0
64	152346.0
65	152346.0
66	125100.5
67	97855.0
68	97855.0
69	97855.0
70	74036.5
71	50218.0
72	50218.0
73	50218.0
74	38433.5
75	26649.0
76	26649.0
77	26649.0
78	21104.5
79	15560.0
80	15560.0
81	15560.0
82	10687.5
83	5815.0
84	5815.0
85	5815.0
86	4493.5
87	3172.0
88	3172.0
89	3172.0
90	2255.0
91	1338.0
92	1338.0
93	1338.0
94	953.5
95	569.0
96	569.0
97	569.0
98	826.5
99	1084.0
100	1084.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03884004162197297
2	0.006045350830968245
3	0.001818955117282481
4	0.00342391551488467
5	0.008934279546652187
6	0.012251197701696711
7	0.020971482528668605
8	0.03429265382876678
9	0.046597350210383554
10	0.05563862711687589
11	0.05151922876303028
12	0.060507006989602534
13	0.06173747662776421
14	0.06467990402336822
15	0.05719008883455801
16	0.06264695418640545
17	0.05943703339120108
18	0.07473765584834195
19	0.07275820469129925
20	0.07661010964554449
21	0.07249071129169889
22	0.07537964000738281
23	0.07960603572106859
24	0.07388167696962077
25	0.07570063208690325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1869205.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.505089547307946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25418473462243	39.27478613113691
2	13.657419072396348	14.06853184617771
3	4.222825822128321	6.524910663342104
4	1.8837058707635659	3.880817582178686
5	1.025778848557613	2.6416415725347147
6	0.6063473154636814	1.8737983667836016
7	0.41175368313108013	1.4845187224770102
8	0.3070377162672767	1.2651204056597614
9	0.22130669245741777	1.025857891119406
>10	1.2486346730650302	11.449284848275221
>50	0.07832745801575586	2.7907545168900336
>100	0.06922939017018012	7.647019858218577
>500	0.009695590972160243	3.359110878442004
>1k	0.0037531319892233203	2.7138467167642397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3554	0.19013430843593934	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2346	0.1255079030924912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6049603976021892E-4
2	0.0	0.0	0.0	0.0	1.6049603976021892E-4
3	0.0	0.0	0.0	0.0	1.6049603976021892E-4
4	0.0	0.0	0.0	0.0	1.6049603976021892E-4
5	0.0	0.0	0.0	0.0	1.6049603976021892E-4
6	0.0	0.0	0.0	0.0	2.1399471968029188E-4
7	0.0	0.0	0.0	0.0	2.1399471968029188E-4
8	0.0	0.0	0.0	0.0	2.1399471968029188E-4
9	0.0	0.0	0.0	0.0	2.1399471968029188E-4
10	0.0	0.0	0.0	0.0	2.1399471968029188E-4
11	0.0	0.0	0.0	5.349867992007297E-5	2.1399471968029188E-4
12	0.0	0.0	0.0	5.349867992007297E-5	4.8148811928065677E-4
13	0.0	0.0	0.0	5.349867992007297E-5	4.8148811928065677E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACCG	40	0.0052831373	14.248574	5
CGTTATT	205	0.0	12.970145	2
GTTATTC	230	0.0	12.800669	3
ACAACGC	75	1.4780406E-5	12.667434	8
GCACTAT	75	1.4796689E-5	12.666078	1
GCGTTAT	230	0.0	12.390729	1
GTATCAA	2470	0.0	12.191741	1
TATTCCC	275	0.0	12.0897	5
CGAACGA	160	3.6379788E-12	11.876355	16
ACTCTAA	250	0.0	11.780714	10
GTATTAG	220	0.0	11.658548	1
AATCGCT	155	2.5465852E-11	11.646491	15
ACCGTCG	335	0.0	11.627569	8
CCGTCGT	330	0.0	11.515849	9
CGTCGTA	330	0.0	11.515849	10
CGATAAC	165	7.2759576E-12	11.515849	10
ATAACGA	165	7.2759576E-12	11.515541	12
CGGACCA	355	0.0	11.50774	9
AATTCCG	190	0.0	11.49885	5
TGAACTG	200	0.0	11.39886	5
>>END_MODULE