FastQCFastQC Report
Thu 2 Feb 2017
SRR4064119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869205
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19100.10218247864733937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28700.023.2277531
TAGATCG703.2178636E-521.998855
TACCGTC6150.018.6006557
ACCGTCG5700.018.5253478
TATACCG851.4319358E-418.11675
ATACCGT6000.017.9657276
GTAATAC2055.456968E-1217.1693743
CGTCGTA6050.016.72639810
AACGCAG40150.016.4922876
ACGCTTA953.3488707E-416.20967917
ACTAGAC1501.8407991E-716.132063
CCGTCGT6300.016.062659
CGTATAT3750.015.83917210
GTATTAC1958.913048E-1015.7957351
GGTATCA14500.015.7801751
TAATACT3650.015.6704134
CGACGGT4700.015.4460017
TAATACC2152.1645974E-1015.3480354
TCAACGC43400.015.2572674
TATCAAC44700.015.2560642
Produced by FastQC (version 0.11.5)