##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064119_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1869205 Sequences flagged as poor quality 0 Sequence length 50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.349494571221456 32.0 32.0 32.0 32.0 32.0 2 31.43680602181141 32.0 32.0 32.0 32.0 32.0 3 31.50419616895953 32.0 32.0 32.0 32.0 32.0 4 31.579949764739556 32.0 32.0 32.0 32.0 32.0 5 31.53649118208008 32.0 32.0 32.0 32.0 32.0 6 35.13877985560706 36.0 36.0 36.0 36.0 36.0 7 35.15628515866371 36.0 36.0 36.0 36.0 36.0 8 35.10012063952322 36.0 36.0 36.0 36.0 36.0 9 35.20332601293063 36.0 36.0 36.0 36.0 36.0 10 35.06058190514149 36.0 36.0 36.0 36.0 36.0 11 35.20503315580688 36.0 36.0 36.0 36.0 36.0 12 35.1084343343828 36.0 36.0 36.0 36.0 36.0 13 35.15252580642573 36.0 36.0 36.0 36.0 36.0 14 35.08932514090215 36.0 36.0 36.0 36.0 36.0 15 35.06915078870429 36.0 36.0 36.0 36.0 36.0 16 35.07967718896536 36.0 36.0 36.0 36.0 36.0 17 35.039223627157 36.0 36.0 36.0 36.0 36.0 18 35.03487899936069 36.0 36.0 36.0 36.0 36.0 19 35.03232390240771 36.0 36.0 36.0 36.0 36.0 20 35.02754754026444 36.0 36.0 36.0 36.0 36.0 21 35.02139465708684 36.0 36.0 36.0 36.0 36.0 22 35.01023376248191 36.0 36.0 36.0 36.0 36.0 23 34.94851501039212 36.0 36.0 36.0 36.0 36.0 24 34.923852118948965 36.0 36.0 36.0 36.0 36.0 25 34.903760154718185 36.0 36.0 36.0 32.0 36.0 26 34.84119505351205 36.0 36.0 36.0 32.0 36.0 27 34.837888835093 36.0 36.0 36.0 32.0 36.0 28 34.80871439997218 36.0 36.0 36.0 32.0 36.0 29 34.783541666109386 36.0 36.0 36.0 32.0 36.0 30 34.7666676474758 36.0 36.0 36.0 32.0 36.0 31 34.7587685673856 36.0 36.0 36.0 32.0 36.0 32 34.72051166137476 36.0 36.0 36.0 32.0 36.0 33 34.697064794926185 36.0 36.0 36.0 32.0 36.0 34 34.69059038468226 36.0 36.0 36.0 32.0 36.0 35 34.650719423498224 36.0 36.0 36.0 32.0 36.0 36 34.628391214446786 36.0 36.0 36.0 32.0 36.0 37 34.61754328711939 36.0 36.0 36.0 32.0 36.0 38 34.57883217731602 36.0 36.0 36.0 32.0 36.0 39 34.591737128886344 36.0 36.0 36.0 32.0 36.0 40 34.55948223977573 36.0 36.0 36.0 32.0 36.0 41 34.54405322048679 36.0 36.0 36.0 32.0 36.0 42 34.47917751129491 36.0 36.0 36.0 32.0 36.0 43 34.47559737963466 36.0 36.0 36.0 32.0 36.0 44 34.40597526756027 36.0 36.0 36.0 32.0 36.0 45 34.38878507172836 36.0 36.0 36.0 32.0 36.0 46 34.37176660665898 36.0 36.0 36.0 32.0 36.0 47 34.33414954485998 36.0 36.0 36.0 32.0 36.0 48 34.29881366677277 36.0 36.0 36.0 32.0 36.0 49 34.27622866405771 36.0 36.0 36.0 32.0 36.0 50 33.681938578165585 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 3.0 18 0.0 19 6.0 20 14.0 21 63.0 22 229.0 23 601.0 24 1484.0 25 3544.0 26 7012.0 27 12626.0 28 20699.0 29 31661.0 30 45271.0 31 63874.0 32 92022.0 33 148227.0 34 315101.0 35 1126768.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.336284748323614 17.363929317989328 11.236849581631708 27.06293635205535 2 16.48765941328848 20.21754784672357 36.48538036098905 26.80941237899891 3 18.65568157500535 23.983629360154076 28.10410870960839 29.256580355232188 4 12.5944452320639 15.694800730791968 35.33887401328372 36.371880023860406 5 14.638041306330765 36.65670699575488 32.979956719567944 15.72529497834641 6 34.81519406121536 34.95188326153807 16.599686283573114 13.633236393673457 7 30.74793829462258 29.950807963813492 20.515245786310224 18.7860079552537 8 28.375822007656737 32.70959189045177 19.07052413754732 19.84406196434418 9 27.96204752886292 13.753678083437656 17.833862977348357 40.45041141035106 10 16.060779365574447 26.366222320185194 31.267765076474426 26.30523323776594 11 38.003910753502154 20.559703189323802 22.085432041964363 19.350954015209677 12 25.344719297325813 23.132545332179184 28.35086844546496 23.17186692503004 13 30.036213411427475 19.044327590938785 24.74442233750643 26.175036660127315 14 24.119420392200922 18.99775036847413 24.69009386393608 32.19273537538887 15 25.736430624728147 26.569176782405812 21.719560626755843 25.9748319661102 16 26.358864726894925 25.0660045613365 23.186258853438577 25.388871858330003 17 24.787289134840286 25.198159216436515 24.593570311212687 25.42098133751051 18 25.527164873472408 24.335797671406493 25.461789206809126 24.675248248311977 19 26.271236312355416 24.240906824509256 24.74208272612299 24.745774137012333 20 26.559201452604707 23.607394417058902 24.254090265643907 25.579313864692484 21 27.52127241260322 23.550707386295244 23.876086357569125 25.05193384353241 22 26.62954565638171 23.716216866876884 24.27752591747708 25.376711559264326 23 25.17737553753 23.539507318111834 24.835571191494964 26.447545952863205 24 25.467847207361437 24.25208645409801 24.586454098009845 25.69361224053071 25 25.67845476130098 23.653977639320896 24.750183771013294 25.917383828364827 26 25.38586443316757 24.478536633647472 24.954216300227646 25.181382632957312 27 25.955831290467206 24.168720233095396 24.220777205787154 25.654671270650248 28 25.349895780278292 24.00916363138319 24.898990331320274 25.74195025701825 29 25.31318814186449 24.160426800430567 24.84945141250313 25.67693364520181 30 25.121820469690938 24.468794271602402 24.970307263645818 25.43907799506084 31 25.880550236559348 24.18576556361475 23.94051213239405 25.993172067431853 32 25.547793204988828 24.312088579964218 23.97241292233417 26.167705292712785 33 25.233035851381363 23.872351512706693 24.631634024973074 26.26297861093887 34 25.915063450961927 23.905818656508806 24.702540178475893 25.476577714053374 35 26.343330694001583 23.878105673136595 24.75341864794881 25.025144984913013 36 25.34124105165163 24.503761248970072 24.386750559104577 25.76824714027372 37 26.235296761194682 24.24829307073926 24.150387396169158 25.3660227718969 38 25.406136508092768 24.13149599053222 24.604617468738564 25.857750032636446 39 26.068110395036868 23.986246095802244 24.13711745817065 25.80852605099024 40 26.2365789289251 24.166461138612934 24.60965273873042 24.987307193731546 41 25.313764440333266 24.532984655649578 24.844836469060652 25.308414434956504 42 26.382812989018174 24.615834010546877 24.312064280628018 24.68928871980693 43 25.721532585661848 23.796411369471443 24.60997624093119 25.87207980393552 44 25.376607785421037 24.333319423535166 24.30560682564496 25.98446596539884 45 25.555362363810968 24.502443578350995 24.39319810505648 25.54899595278156 46 25.448468938199532 24.291392060637936 24.433110973559177 25.827028027603355 47 25.672890806313376 24.054903395831527 24.788649959821825 25.483555838033272 48 26.2342871939293 24.59725568913789 23.884374719127575 25.284082397805236 49 25.398874489954775 24.716495907065976 24.086225261435793 25.798404341543453 50 25.50499443873487 24.940629014128664 24.08528022195841 25.469096325178057 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 20.0 1 26.0 2 32.0 3 81.0 4 130.0 5 167.0 6 204.0 7 217.5 8 231.0 9 295.0 10 359.0 11 529.5 12 700.0 13 1324.5 14 1949.0 15 2597.5 16 3246.0 17 3479.5 18 3713.0 19 3790.5 20 3868.0 21 4175.0 22 4482.0 23 5117.0 24 5752.0 25 6679.0 26 7606.0 27 9536.0 28 11466.0 29 13803.0 30 16140.0 31 19201.5 32 22263.0 33 26436.0 34 30609.0 35 36271.0 36 41933.0 37 50207.5 38 58482.0 39 65204.5 40 71927.0 41 80223.0 42 88519.0 43 92923.0 44 97327.0 45 107679.0 46 118031.0 47 127574.0 48 137117.0 49 143454.5 50 149792.0 51 148051.0 52 146310.0 53 146110.0 54 145910.0 55 145978.0 56 146046.0 57 143299.5 58 140553.0 59 128338.0 60 116123.0 61 102099.5 62 88076.0 63 76615.0 64 65154.0 65 55197.0 66 45240.0 67 39092.5 68 32945.0 69 29455.0 70 25965.0 71 20300.5 72 14636.0 73 12418.5 74 10201.0 75 8012.0 76 5823.0 77 4967.5 78 4112.0 79 3392.0 80 2672.0 81 2115.5 82 1559.0 83 1325.0 84 1091.0 85 757.0 86 423.0 87 310.0 88 197.0 89 152.0 90 107.0 91 86.5 92 66.0 93 48.0 94 30.0 95 36.0 96 42.0 97 27.5 98 13.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00845279142737153 2 3.744907594405108E-4 3 2.674933996003649E-4 4 0.0 5 0.0 6 5.349867992007297E-5 7 0.0 8 5.349867992007297E-5 9 0.0013374669980018242 10 9.629762385613134E-4 11 0.0 12 1.604960397602189E-4 13 4.2798943936058376E-4 14 5.349867992007298E-4 15 0.004707883832966422 16 8.559788787211675E-4 17 5.349867992007297E-5 18 4.2798943936058376E-4 19 5.349867992007297E-5 20 5.884854791208027E-4 21 0.0 22 1.604960397602189E-4 23 3.744907594405108E-4 24 2.674933996003649E-4 25 0.0016584590775222622 26 0.004814881192806568 27 0.0052963693120872245 28 0.004119398353845618 29 0.0021934458767229915 30 0.0032634194751244515 31 0.006312844230568611 32 0.0036914089144850348 33 0.0038519049542452537 34 0.002407440596403284 35 0.002407440596403284 36 0.007757308588410582 37 0.0031564221152843054 38 0.0069013297096894135 39 0.0033704168349645972 40 0.0019259524771226269 41 0.0025679366361635023 42 0.0013909656779218973 43 0.0012839683180817512 44 0.0010164749184813865 45 0.0010699735984014596 46 6.419841590408756E-4 47 0.0016584590775222622 48 0.0015514617176821163 49 4.2798943936058376E-4 50 0.0013909656779218973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1869205.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.344344265851305 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95803837643228 41.82234131553433 2 13.541181056215626 14.717732101704122 3 4.107407296713961 6.696430685180796 4 1.756598457476316 3.818447652398252 5 0.9551928235427785 2.5954663821439654 6 0.5689360315603711 1.8551073338618433 7 0.3788075472283709 1.441023342995692 8 0.28246134731253336 1.2280141360118806 9 0.21319235673687084 1.0427218946421024 >10 1.0996149047135508 10.543635302897798 >50 0.07091676420708033 2.672963332219341 >100 0.056956232595026655 6.671102237303911 >500 0.008220692935327699 3.0879448602068904 >1k 0.0024761123299179813 1.8070694228991795 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 1910 0.10218247864733937 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0699735984014594E-4 2 0.0 0.0 0.0 0.0 1.0699735984014594E-4 3 0.0 0.0 0.0 0.0 1.0699735984014594E-4 4 0.0 0.0 0.0 0.0 1.0699735984014594E-4 5 0.0 0.0 0.0 0.0 1.0699735984014594E-4 6 0.0 0.0 0.0 0.0 1.0699735984014594E-4 7 0.0 0.0 0.0 5.349867992007297E-5 1.0699735984014594E-4 8 0.0 0.0 0.0 5.349867992007297E-5 1.0699735984014594E-4 9 0.0 0.0 0.0 5.349867992007297E-5 1.0699735984014594E-4 10 0.0 0.0 0.0 5.349867992007297E-5 1.6049603976021892E-4 11 0.0 0.0 0.0 1.0699735984014594E-4 1.6049603976021892E-4 12 0.0 0.0 0.0 1.0699735984014594E-4 3.2099207952043785E-4 13 0.0 0.0 0.0 1.0699735984014594E-4 3.2099207952043785E-4 14 0.0 0.0 0.0 1.0699735984014594E-4 3.2099207952043785E-4 15 0.0 0.0 0.0 1.0699735984014594E-4 5.349867992007297E-4 16 0.0 0.0 0.0 1.0699735984014594E-4 5.884854791208027E-4 17 0.0 0.0 0.0 1.0699735984014594E-4 5.884854791208027E-4 18 0.0 0.0 0.0 2.1399471968029188E-4 5.884854791208027E-4 19 0.0 0.0 0.0 2.6749339960036484E-4 5.884854791208027E-4 20 0.0 0.0 0.0 3.744907594405108E-4 7.489815188810216E-4 21 0.0 0.0 0.0 4.8148811928065677E-4 8.024801988010946E-4 22 0.0 0.0 0.0 6.954828389609487E-4 8.559788787211675E-4 23 0.0 0.0 0.0 0.0010699735984014593 8.559788787211675E-4 24 0.0 0.0 0.0 0.0015514617176821163 8.559788787211675E-4 25 0.0 0.0 0.0 0.002193445876722992 8.559788787211675E-4 26 0.0 0.0 0.0 0.002407440596403284 9.629762385613135E-4 27 0.0 0.0 0.0 0.0029424273956040136 0.0010164749184813865 28 0.0 0.0 0.0 0.004012400994005473 0.0010164749184813865 29 0.0 0.0 0.0 0.005510364031767516 0.0010699735984014593 30 0.0 0.0 0.0 0.010004253145053646 0.0010699735984014593 31 0.0 0.0 0.0 0.025518870321874807 0.0010699735984014593 32 0.0 0.0 0.0 0.04231745581677772 0.0010699735984014593 33 0.0 0.0 0.0 0.059009043951840486 0.0010699735984014593 34 0.0 0.0 0.0 0.07596812548650363 0.0012304696381616783 35 0.0 0.0 0.0 0.09822357633325397 0.0012304696381616783 36 0.0 0.0 0.0 0.1270593648101733 0.0012304696381616783 37 0.0 0.0 0.0 0.17312172822135613 0.0012304696381616783 38 0.0 0.0 0.0 0.23999507812144735 0.0012304696381616783 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2870 0.0 23.227753 1 TAGATCG 70 3.2178636E-5 21.99885 5 TACCGTC 615 0.0 18.600655 7 ACCGTCG 570 0.0 18.525347 8 TATACCG 85 1.4319358E-4 18.1167 5 ATACCGT 600 0.0 17.965727 6 GTAATAC 205 5.456968E-12 17.169374 3 CGTCGTA 605 0.0 16.726398 10 AACGCAG 4015 0.0 16.492287 6 ACGCTTA 95 3.3488707E-4 16.209679 17 ACTAGAC 150 1.8407991E-7 16.13206 3 CCGTCGT 630 0.0 16.06265 9 CGTATAT 375 0.0 15.839172 10 GTATTAC 195 8.913048E-10 15.795735 1 GGTATCA 1450 0.0 15.780175 1 TAATACT 365 0.0 15.670413 4 CGACGGT 470 0.0 15.446001 7 TAATACC 215 2.1645974E-10 15.348035 4 TCAACGC 4340 0.0 15.257267 4 TATCAAC 4470 0.0 15.256064 2 >>END_MODULE