FastQCFastQC Report
Thu 2 Feb 2017
SRR4064118_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064118_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137702
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46290.4068728014893179No Hit
TATCAACGCAGAGTACTTTTTTTTT35190.30930770975176275No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31170.2739733251765401No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20020.1759687510437707No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16500.14502919042069012No Hit
GGTATCAACGCAGAGTACTTTTTTT16110.14160122773801928No Hit
GTACATGGGGTGGTATCAACGCAAA15140.13307526927086355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT401.2872551E-518.9956464
TCGAACG453.5485125E-516.882793
AACGTCT602.5672869E-514.2498686
AACCGCG1153.765308E-1013.2178517
CGCATCG1600.013.06410313
CGAGCCG1550.012.8736815
GCGTAAC751.477896E-512.6665511
ATGGGTT604.0953996E-412.6648785
TAATACC1051.9976142E-812.6637654
CTAGACC751.4812422E-512.6637634
ATATATC751.48325325E-512.6620933
ATCAGTA604.1106227E-412.6593092
ACGGTAT1457.2759576E-1212.448169
AGTGACG1650.012.09398919
CGCCAGT1750.011.94590118
TCGCGTA802.8650442E-511.8748899
AATACCT658.0300216E-411.6906575
TTAATAC907.4855543E-611.6069183
CTCTTAT907.4855543E-611.6069181
GAATAAG907.4855543E-611.6069181
Produced by FastQC (version 0.11.5)