FastQCFastQC Report
Thu 2 Feb 2017
SRR4064118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137702
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.12586775798935046No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11510.10116884737831174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGA602.8728077E-421.99980210
CGGTACT600.007415982418.3315584
ACCGTCG2650.018.262388
GTATTAG2750.017.5982951
CGTCGTA2900.017.44811810
TAATACT1903.274181E-1117.3667374
CCGTCGT2800.017.2855599
TACCGTC2800.017.284047
ATACCGT2850.016.9808126
CGAACTC1602.1300366E-816.49912620
TACCGTG800.00198954116.4984027
CGCAATA2700.016.29758336
GTATAGT953.3485677E-416.2089561
GTATCAA33500.016.0223271
ATCGCTC1653.111745E-815.99915316
ATGGGTG6750.015.9688235
GTATAGA1259.447893E-615.8384651
GTAAGAC1259.447893E-615.8384653
TAGACAG2650.015.7720575
GTATTAC1401.5875303E-615.7127641
Produced by FastQC (version 0.11.5)