##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064118_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1137702
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260647339988854	32.0	32.0	32.0	32.0	32.0
2	31.438568271832168	32.0	32.0	32.0	32.0	32.0
3	31.496565005599006	32.0	32.0	32.0	32.0	32.0
4	31.58251721452542	32.0	32.0	32.0	32.0	32.0
5	31.532069030378782	32.0	32.0	32.0	32.0	32.0
6	35.118799123144726	36.0	36.0	36.0	36.0	36.0
7	35.13860747366182	36.0	36.0	36.0	36.0	36.0
8	35.098323638351694	36.0	36.0	36.0	36.0	36.0
9	35.181032467201426	36.0	36.0	36.0	36.0	36.0
10	35.048512703678114	36.0	36.0	36.0	36.0	36.0
11	35.18577448224579	36.0	36.0	36.0	36.0	36.0
12	35.09496950871142	36.0	36.0	36.0	36.0	36.0
13	35.13102024959084	36.0	36.0	36.0	36.0	36.0
14	35.07134557203908	36.0	36.0	36.0	36.0	36.0
15	35.058153189499535	36.0	36.0	36.0	36.0	36.0
16	35.061518745682086	36.0	36.0	36.0	36.0	36.0
17	35.0199085525032	36.0	36.0	36.0	36.0	36.0
18	35.00843103026979	36.0	36.0	36.0	36.0	36.0
19	35.012344181516774	36.0	36.0	36.0	36.0	36.0
20	34.99560078122391	36.0	36.0	36.0	36.0	36.0
21	35.000180187782036	36.0	36.0	36.0	36.0	36.0
22	34.99654391044403	36.0	36.0	36.0	36.0	36.0
23	34.94248318100873	36.0	36.0	36.0	36.0	36.0
24	34.909344450480006	36.0	36.0	36.0	32.0	36.0
25	34.881934812455285	36.0	36.0	36.0	32.0	36.0
26	34.82502799502858	36.0	36.0	36.0	32.0	36.0
27	34.82106825864769	36.0	36.0	36.0	32.0	36.0
28	34.798028833560984	36.0	36.0	36.0	32.0	36.0
29	34.760066344262384	36.0	36.0	36.0	32.0	36.0
30	34.73571901956751	36.0	36.0	36.0	32.0	36.0
31	34.7285668830678	36.0	36.0	36.0	32.0	36.0
32	34.683601681283854	36.0	36.0	36.0	32.0	36.0
33	34.63991801016435	36.0	36.0	36.0	32.0	36.0
34	34.63127954420402	36.0	36.0	36.0	32.0	36.0
35	34.57765917612872	36.0	36.0	36.0	32.0	36.0
36	34.54544335862994	36.0	36.0	36.0	32.0	36.0
37	34.53900669946963	36.0	36.0	36.0	32.0	36.0
38	34.49159094384997	36.0	36.0	36.0	32.0	36.0
39	34.489117536929704	36.0	36.0	36.0	32.0	36.0
40	34.4601908056767	36.0	36.0	36.0	32.0	36.0
41	34.4327706200745	36.0	36.0	36.0	32.0	36.0
42	34.36934979458593	36.0	36.0	36.0	32.0	36.0
43	34.34140310907426	36.0	36.0	36.0	32.0	36.0
44	34.26994415057722	36.0	36.0	36.0	32.0	36.0
45	34.22669820392335	36.0	36.0	36.0	32.0	36.0
46	34.177803150561395	36.0	36.0	36.0	32.0	36.0
47	34.129077737403996	36.0	36.0	36.0	32.0	36.0
48	34.06989791702924	36.0	36.0	36.0	32.0	36.0
49	34.040321630796115	36.0	36.0	36.0	32.0	36.0
50	33.4582263193701	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	17.0
21	62.0
22	162.0
23	452.0
24	1136.0
25	2502.0
26	4941.0
27	8945.0
28	14397.0
29	21471.0
30	29977.0
31	40926.0
32	57942.0
33	90652.0
34	185110.0
35	679005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21018765059208	17.73718720139804	11.733897800004044	26.318727348005833
2	16.023099336816983	20.884331916726364	36.85486883567213	26.237699910784524
3	18.19009647568419	24.8387970072849	28.71056943155289	28.26053708547802
4	12.306825513183593	16.184202893200506	35.7051319238254	35.803839669790506
5	14.16794556043674	37.077987029995555	33.184964076709015	15.569103332858692
6	33.60601774983058	35.664906684621	17.08076199282588	13.648313572722534
7	29.82338944942476	30.76335522250574	21.026438405169724	18.38681692289978
8	28.120896333134688	32.312591522208805	19.708148530986147	19.858363613670363
9	27.856719692428317	14.036210342748351	18.4911302415605	39.615939723262834
10	15.949276075690348	26.799438861972703	31.643473430325365	25.60781163201159
11	36.99351851363538	21.184985171863985	22.670523564167066	19.150972750333565
12	25.15001744746643	23.88536179541022	28.619294524659818	22.345326232463538
13	29.669215982195517	19.597764243542404	25.27461322977856	25.45840654448352
14	23.860068559374177	19.477542410125693	25.789048079458553	30.873340951041573
15	25.259964804764895	26.818610930915725	22.978867036902255	24.942557227417122
16	25.503123868322884	25.639979889108826	23.932927365227144	24.923968877341142
17	23.83356977486196	25.516611555574308	25.47468493507087	25.175133734492867
18	24.51612733102692	24.68506525469018	26.587067195454672	24.21174021882823
19	25.26865558819445	24.632900355277567	26.192095997018548	23.906348059509433
20	25.90230765038754	23.747774006015675	25.662172781081733	24.68774556251505
21	26.836552981360672	23.924718423629386	25.179001179570747	24.05972741543919
22	25.837456414295147	24.08998497847853	25.512678638763614	24.559879968462717
23	24.561371125602424	23.973954309290818	25.965176897458274	25.49949766764848
24	24.621669917385734	24.635118137781852	25.929223686095682	24.813988258736728
25	24.86454938278116	24.17314473966409	26.072969295516152	24.8893365820386
26	24.56358718630975	24.974794022996992	26.183889798677423	24.277728992015838
27	25.079113367697836	24.520836702976773	25.552387114014667	24.847662815310724
28	24.408885419715745	24.527376434879606	26.201984988208032	24.861753157196613
29	24.321917766075362	24.847207901854425	26.101100771146314	24.7297735609239
30	24.36990449569066	24.919900320398717	26.26012508295646	24.450070100954164
31	24.773385747035906	24.773913165778257	25.357238294820046	25.09546279236579
32	24.64938596946153	24.852876155784703	25.28613884623262	25.21159902852115
33	24.424791518515228	24.4201327143863	25.782788971116293	25.37228679598218
34	24.965345198738802	24.56205736567254	25.82307899888278	24.649518436705872
35	25.5439927640502	24.400586118965975	25.904999151762443	24.15042196522138
36	24.506987529865558	25.089442843605564	25.44818116768841	24.95538845884047
37	25.151849175751245	25.0863628452536	25.385050833214667	24.376737145780485
38	24.397797145783066	24.905701841132544	25.783854819073238	24.912646194011153
39	24.937809523976956	24.889463570884836	25.318863536075746	24.853863369062456
40	25.253961822003024	24.966620402559265	25.576990184332725	24.202427591104986
41	24.250742752667755	25.343687920819928	25.90273016542728	24.502839161085035
42	25.251696877323816	25.420110083872586	25.361569793913567	23.96662324489003
43	24.67260327313224	24.57732223361962	25.57724576267462	25.17282873057352
44	24.435762095834964	25.2627045271758	25.25242048545951	25.049112891529727
45	24.54128258926256	25.393628981007893	25.22249167826187	24.842596751467678
46	24.308531256839537	25.188650305400344	25.289205203188576	25.213613234571547
47	24.30595354037529	25.207792025146	25.659238658458122	24.827015776020588
48	25.140592889753023	25.548791314268836	24.816688670472065	24.493927125506072
49	24.3381360222766	25.704493994880885	24.852508930329368	25.10486105251315
50	24.408974724088587	25.971798847483136	24.846881910970005	24.772344517458276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	270.0
1	173.0
2	76.0
3	241.0
4	406.0
5	450.5
6	495.0
7	441.0
8	387.0
9	526.5
10	666.0
11	992.5
12	1319.0
13	2459.5
14	3600.0
15	4531.5
16	5463.0
17	5636.5
18	5810.0
19	5747.0
20	5684.0
21	5859.5
22	6035.0
23	6463.0
24	6891.0
25	7014.5
26	7138.0
27	8431.0
28	9724.0
29	11346.0
30	12968.0
31	15272.5
32	17577.0
33	20386.0
34	23195.0
35	26423.5
36	29652.0
37	34760.0
38	39868.0
39	43544.0
40	47220.0
41	52290.0
42	57360.0
43	59718.5
44	62077.0
45	68322.0
46	74567.0
47	78785.0
48	83003.0
49	85124.0
50	87245.0
51	85124.5
52	83004.0
53	82670.5
54	82337.0
55	81498.5
56	80660.0
57	78481.0
58	76302.0
59	69582.5
60	62863.0
61	55349.0
62	47835.0
63	41885.0
64	35935.0
65	30589.5
66	25244.0
67	21579.0
68	17914.0
69	15963.5
70	14013.0
71	11010.0
72	8007.0
73	6875.0
74	5743.0
75	4521.5
76	3300.0
77	2817.5
78	2335.0
79	1921.0
80	1507.0
81	1203.5
82	900.0
83	753.0
84	606.0
85	426.0
86	246.0
87	179.5
88	113.0
89	81.5
90	50.0
91	45.0
92	40.0
93	26.5
94	13.0
95	15.5
96	18.0
97	14.5
98	11.0
99	10.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009580716215669834
2	6.152753532998975E-4
3	5.27378874257055E-4
4	0.0
5	0.0
6	8.789647904284249E-5
7	8.789647904284249E-5
8	0.0
9	0.0014063436646854798
10	0.001230550706599795
11	0.0
12	8.789647904284249E-5
13	9.668612694712674E-4
14	1.7579295808568498E-4
15	0.00421903099405644
16	8.78964790428425E-4
17	0.0
18	5.27378874257055E-4
19	0.0
20	7.031718323427399E-4
21	0.0
22	8.789647904284249E-5
23	7.910683113855825E-4
24	4.394823952142125E-4
25	0.001582136622771165
26	0.006592235928213187
27	0.008262269030027195
28	0.005537478179699078
29	0.003955341556927913
30	0.005010099305442023
31	0.007559097197684455
32	0.004834306347356337
33	0.006240650012041818
34	0.004658513389270653
35	0.004306927473099283
36	0.00914123382045562
37	0.00527378874257055
38	0.007646993676727298
39	0.0059769605749132905
40	0.0027247908503281178
41	0.0036916521197993854
42	0.00210951549702822
43	0.0013184471856426375
44	0.0014942401437283226
45	0.0016700331018140075
46	0.0013184471856426375
47	0.002285308455113905
48	0.00175792958085685
49	5.27378874257055E-4
50	0.001582136622771165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1137702.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.424601649539746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41859026126153	45.23150296283142
2	13.651290819255932	15.951424562340938
3	4.278755403200293	7.4995373996337875
4	1.695348153097625	3.9620016200804673
5	0.8805035632697529	2.572153496751781
6	0.5022243691832364	1.7605355224933184
7	0.3242526456357349	1.326103215855403
8	0.22399432307001801	1.046942327769929
9	0.14831491384422035	0.7798715784030944
>10	0.7484257888496767	7.617092265537156
>50	0.05970284433924131	2.4574791213583094
>100	0.0625397960705397	7.238096178739825
>500	0.005602835003038019	2.234959202876467
>1k	4.5428391916524477E-4	0.32230054532812
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1432	0.12586775798935046	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1151	0.10116884737831174	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.78964790428425E-5
2	0.0	0.0	0.0	0.0	8.78964790428425E-5
3	0.0	0.0	0.0	0.0	8.78964790428425E-5
4	0.0	0.0	0.0	0.0	8.78964790428425E-5
5	0.0	0.0	0.0	1.75792958085685E-4	8.78964790428425E-5
6	0.0	0.0	0.0	1.75792958085685E-4	8.78964790428425E-5
7	0.0	0.0	0.0	1.75792958085685E-4	8.78964790428425E-5
8	0.0	0.0	0.0	2.636894371285275E-4	8.78964790428425E-5
9	0.0	0.0	0.0	2.636894371285275E-4	8.78964790428425E-5
10	0.0	0.0	0.0	2.636894371285275E-4	8.78964790428425E-5
11	0.0	0.0	0.0	2.636894371285275E-4	8.78964790428425E-5
12	0.0	0.0	0.0	2.636894371285275E-4	5.27378874257055E-4
13	0.0	0.0	0.0	2.636894371285275E-4	6.152753532998976E-4
14	0.0	0.0	0.0	2.636894371285275E-4	6.152753532998976E-4
15	0.0	0.0	0.0	2.636894371285275E-4	7.0317183234274E-4
16	0.0	0.0	0.0	2.636894371285275E-4	7.0317183234274E-4
17	0.0	0.0	0.0	2.636894371285275E-4	7.0317183234274E-4
18	0.0	0.0	0.0	2.636894371285275E-4	7.0317183234274E-4
19	0.0	0.0	0.0	2.636894371285275E-4	7.0317183234274E-4
20	0.0	0.0	0.0	3.5158591617137E-4	0.0013184471856426375
21	0.0	0.0	0.0	4.3948239521421253E-4	0.00140634366468548
22	0.0	0.0	0.0	7.910683113855825E-4	0.001582136622771165
23	0.0	0.0	0.0	0.00210951549702822	0.001582136622771165
24	0.0	0.0	0.0	0.00421903099405644	0.0016700331018140075
25	0.0	0.0	0.0	0.005185892263527708	0.0016700331018140075
26	0.0	0.0	0.0	0.00632854649108466	0.0020216190179853776
27	0.0	0.0	0.0	0.00843806198811288	0.0021974119760710628
28	0.0	0.0	0.0	0.012041817628869423	0.002285308455113905
29	0.0	0.0	0.0	0.018809846515168295	0.0023732049341567475
30	0.0	0.0	0.0	0.036301245844693956	0.0023732049341567475
31	0.0	0.0	0.0	0.07752469451578708	0.0023732049341567475
32	0.0	0.0	0.0	0.12156083051625118	0.0023732049341567475
33	0.0	0.0	0.0	0.16603644891192948	0.0023732049341567475
34	0.0	0.0	0.0	0.21103944618186485	0.0024611014131995903
35	0.0	0.0	0.0	0.26114043923628505	0.0024611014131995903
36	0.0	0.0	0.0	0.33128182951247337	0.0024611014131995903
37	0.0	0.0	0.0	0.43851553394474124	0.0024611014131995903
38	0.0	0.0	0.0	0.5906643391679016	0.002636894371285275
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGCGA	60	2.8728077E-4	21.999802	10
CGGTACT	60	0.0074159824	18.331558	4
ACCGTCG	265	0.0	18.26238	8
GTATTAG	275	0.0	17.598295	1
CGTCGTA	290	0.0	17.448118	10
TAATACT	190	3.274181E-11	17.366737	4
CCGTCGT	280	0.0	17.285559	9
TACCGTC	280	0.0	17.28404	7
ATACCGT	285	0.0	16.980812	6
CGAACTC	160	2.1300366E-8	16.499126	20
TACCGTG	80	0.001989541	16.498402	7
CGCAATA	270	0.0	16.297583	36
GTATAGT	95	3.3485677E-4	16.208956	1
GTATCAA	3350	0.0	16.022327	1
ATCGCTC	165	3.111745E-8	15.999153	16
ATGGGTG	675	0.0	15.968823	5
GTATAGA	125	9.447893E-6	15.838465	1
GTAAGAC	125	9.447893E-6	15.838465	3
TAGACAG	265	0.0	15.772057	5
GTATTAC	140	1.5875303E-6	15.712764	1
>>END_MODULE