FastQCFastQC Report
Thu 2 Feb 2017
SRR4064117_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064117_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTT210.8684863523573202No Hit
GTATCAACGCAGAGTACTTTTTTTT160.6617038875103392No Hit
GGTTAATTCCGATAACGAACGAGAC100.41356492969396197No Hit
GTACTTTTTTTTTTTTTTTTTTTTT70.2894954507857734No Hit
GGTATCAACGCAGAGTACTTTTTTT60.24813895781637718No Hit
GTGTATATCAATGAGTTACAATGAA50.20678246484698098No Hit
CTGTAGGACGTGGAATATGGCAAGA50.20678246484698098No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG40.1654259718775848No Hit
GTTTATGGTCGGAACTACGACGGTA40.1654259718775848No Hit
CTCCAATGGATCCTCGTTAAAGGAT40.1654259718775848No Hit
GGCTCCACTCCTGGTGGTGCCCTTC40.1654259718775848No Hit
GTGTATATCAATGAGTTACAATGAG40.1654259718775848No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40.1654259718775848No Hit
GTCCTACAGTGTGCATTTCTCATTT40.1654259718775848No Hit
GAATTACCGCGGCTGCTGGCACCAG40.1654259718775848No Hit
ATATTAAAGTTGCTGCAGTTAAAAA40.1654259718775848No Hit
CAACGCAGAGTACTTTTTTTTTTTT40.1654259718775848No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40.1654259718775848No Hit
GCTCCACTCCTGGTGGTGCCCTTCC30.12406947890818859No Hit
CCGGAACCCAAAGACTTTGGTTTCC30.12406947890818859No Hit
GGAGAGGAGCGAGCGACCAAAGGAA30.12406947890818859No Hit
GAATCAGGGTTCGATTCCGGAGAGG30.12406947890818859No Hit
TCTTAGCTGAGTGTCCCGCGGGGCC30.12406947890818859No Hit
GTATCAACGCAGAGTACATGGGGAA30.12406947890818859No Hit
GTACATGGGGAATAATTGCAATCCC30.12406947890818859No Hit
GCGCAAGACGGACCAGAGCGAAAGC30.12406947890818859No Hit
CCATTATTCCTAGCTGCGGTATCCA30.12406947890818859No Hit
GTCAATCCTGTCCGTGTCCGGGCCG30.12406947890818859No Hit
GCTTTGAACACTCTAATTTTTTCAA30.12406947890818859No Hit
AGGTTATCTAGAGTCACCAAAGCCG30.12406947890818859No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG30.12406947890818859No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers