##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064117_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2418
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.426799007444167	32.0	32.0	32.0	14.0	32.0
2	27.67535153019024	32.0	21.0	32.0	14.0	32.0
3	28.038047973531846	32.0	21.0	32.0	14.0	32.0
4	27.709263854425146	32.0	21.0	32.0	14.0	32.0
5	27.77005789909016	32.0	21.0	32.0	14.0	32.0
6	31.44789081885856	36.0	32.0	36.0	21.0	36.0
7	30.99462365591398	36.0	27.0	36.0	14.0	36.0
8	31.064102564102566	36.0	32.0	36.0	14.0	36.0
9	31.1232423490488	36.0	32.0	36.0	14.0	36.0
10	30.20678246484698	36.0	21.0	36.0	14.0	36.0
11	31.766749379652605	36.0	32.0	36.0	14.0	36.0
12	30.697684036393714	36.0	32.0	36.0	14.0	36.0
13	31.11373035566584	36.0	32.0	36.0	14.0	36.0
14	30.74317617866005	36.0	27.0	36.0	14.0	36.0
15	30.86848635235732	36.0	32.0	36.0	14.0	36.0
16	30.563275434243177	36.0	21.0	36.0	14.0	36.0
17	30.4590570719603	36.0	21.0	36.0	14.0	36.0
18	30.522332506203472	36.0	27.0	36.0	14.0	36.0
19	30.488833746898262	36.0	27.0	36.0	14.0	36.0
20	30.24524400330852	36.0	21.0	36.0	14.0	36.0
21	30.557071960297765	36.0	27.0	36.0	14.0	36.0
22	30.55665839536807	36.0	27.0	36.0	14.0	36.0
23	30.13358147229115	36.0	21.0	36.0	14.0	36.0
24	30.149710504549216	36.0	21.0	36.0	14.0	36.0
25	29.591397849462364	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	5.0
16	12.0
17	26.0
18	47.0
19	42.0
20	69.0
21	80.0
22	65.0
23	58.0
24	72.0
25	92.0
26	64.0
27	91.0
28	107.0
29	82.0
30	101.0
31	141.0
32	186.0
33	240.0
34	387.0
35	450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.84532671629446	18.320926385442515	12.200165425971878	26.63358147229115
2	16.335814722911497	20.43010752688172	38.29611248966088	24.937965260545905
3	18.27956989247312	23.118279569892472	30.23159636062862	28.37055417700579
4	12.779156327543426	15.550041356492969	35.194375516956164	36.47642679900744
5	13.316790736145576	37.799834574028125	33.7055417700579	15.177832919768402
6	32.09263854425144	38.0479735318445	15.591397849462366	14.267990074441686
7	27.223831195697144	31.89904840711626	21.762515515101367	19.114604882085228
8	26.768721555647495	34.215970211005384	19.528340918494	19.486967314853125
9	27.265204799338022	14.356640463384359	18.328506412908567	40.04964832436905
10	13.901530823334712	29.00289615225486	30.699213901530825	26.396359122879602
11	38.54425144747725	19.851116625310176	22.870140612076096	18.734491315136477
12	24.493173355399257	24.493173355399257	27.637567232105916	23.376086057095574
13	29.84271523178808	20.736754966887418	24.58609271523179	24.834437086092713
14	23.41745966073645	20.81092263136119	26.106743897393464	29.664873810508897
15	25.279271824575922	28.754654530409603	22.58998758791891	23.376086057095574
16	24.286305337194868	26.27223831195697	26.189491104675216	23.251965246172944
17	22.755482002482417	26.189491104675216	26.768721555647495	24.286305337194868
18	22.80629139072848	24.04801324503311	29.55298013245033	23.59271523178808
19	24.668874172185433	24.296357615894042	26.117549668874172	24.917218543046356
20	24.875827814569536	24.503311258278146	26.40728476821192	24.2135761589404
21	26.065370293752586	23.624327678940833	24.86553578816715	25.444766239139426
22	24.2135761589404	26.531456953642383	26.862582781456958	22.392384105960264
23	23.054635761589402	23.923841059602648	26.945364238410598	26.076158940397352
24	22.4244931733554	27.141083988415392	26.60322714108399	23.831195697145223
25	24.12081092263136	24.90690939180803	27.347952006619774	23.624327678940833
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	2.0
3	4.0
4	4.0
5	4.0
6	5.5
7	7.0
8	7.0
9	7.0
10	7.0
11	7.0
12	7.0
13	7.0
14	6.0
15	5.0
16	5.0
17	5.0
18	11.5
19	18.0
20	18.0
21	18.0
22	29.5
23	41.0
24	41.0
25	41.0
26	83.5
27	126.0
28	126.0
29	126.0
30	136.0
31	146.0
32	146.0
33	146.0
34	164.0
35	182.0
36	182.0
37	182.0
38	187.0
39	192.0
40	192.0
41	192.0
42	214.0
43	236.0
44	236.0
45	236.0
46	257.5
47	279.0
48	279.0
49	279.0
50	291.0
51	303.0
52	303.0
53	303.0
54	285.0
55	267.0
56	267.0
57	267.0
58	236.0
59	205.0
60	205.0
61	205.0
62	186.0
63	167.0
64	167.0
65	167.0
66	137.5
67	108.0
68	108.0
69	108.0
70	84.0
71	60.0
72	60.0
73	60.0
74	43.0
75	26.0
76	26.0
77	26.0
78	24.0
79	22.0
80	22.0
81	22.0
82	16.0
83	10.0
84	10.0
85	10.0
86	7.5
87	5.0
88	5.0
89	5.0
90	3.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0413564929693962
8	0.0413564929693962
9	0.0413564929693962
10	0.0413564929693962
11	0.0
12	0.0413564929693962
13	0.0827129859387924
14	0.0413564929693962
15	0.0413564929693962
16	0.0413564929693962
17	0.0413564929693962
18	0.0827129859387924
19	0.0827129859387924
20	0.0827129859387924
21	0.0413564929693962
22	0.0827129859387924
23	0.0827129859387924
24	0.0413564929693962
25	0.0413564929693962
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2418.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.18362282878412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.60340960071781	88.1306865177833
2	3.00583221175415	5.5417700578990905
3	0.5832211754149843	1.6129032258064515
4	0.493494840735756	1.8196856906534327
5	0.08972633467922835	0.41356492969396197
6	0.04486316733961417	0.24813895781637718
7	0.04486316733961417	0.2894954507857734
8	0.0	0.0
9	0.0	0.0
>10	0.13458950201884254	1.943755169561621
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTT	21	0.8684863523573202	No Hit
GTATCAACGCAGAGTACTTTTTTTT	16	0.6617038875103392	No Hit
GGTTAATTCCGATAACGAACGAGAC	10	0.41356492969396197	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7	0.2894954507857734	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6	0.24813895781637718	No Hit
GTGTATATCAATGAGTTACAATGAA	5	0.20678246484698098	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5	0.20678246484698098	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	4	0.1654259718775848	No Hit
GTTTATGGTCGGAACTACGACGGTA	4	0.1654259718775848	No Hit
CTCCAATGGATCCTCGTTAAAGGAT	4	0.1654259718775848	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	4	0.1654259718775848	No Hit
GTGTATATCAATGAGTTACAATGAG	4	0.1654259718775848	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4	0.1654259718775848	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4	0.1654259718775848	No Hit
GAATTACCGCGGCTGCTGGCACCAG	4	0.1654259718775848	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	4	0.1654259718775848	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	4	0.1654259718775848	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4	0.1654259718775848	No Hit
GCTCCACTCCTGGTGGTGCCCTTCC	3	0.12406947890818859	No Hit
CCGGAACCCAAAGACTTTGGTTTCC	3	0.12406947890818859	No Hit
GGAGAGGAGCGAGCGACCAAAGGAA	3	0.12406947890818859	No Hit
GAATCAGGGTTCGATTCCGGAGAGG	3	0.12406947890818859	No Hit
TCTTAGCTGAGTGTCCCGCGGGGCC	3	0.12406947890818859	No Hit
GTATCAACGCAGAGTACATGGGGAA	3	0.12406947890818859	No Hit
GTACATGGGGAATAATTGCAATCCC	3	0.12406947890818859	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3	0.12406947890818859	No Hit
CCATTATTCCTAGCTGCGGTATCCA	3	0.12406947890818859	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	3	0.12406947890818859	No Hit
GCTTTGAACACTCTAATTTTTTCAA	3	0.12406947890818859	No Hit
AGGTTATCTAGAGTCACCAAAGCCG	3	0.12406947890818859	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	3	0.12406947890818859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE