##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2418
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.062034739454095	32.0	32.0	32.0	21.0	32.0
2	29.992142266335815	32.0	32.0	32.0	21.0	32.0
3	30.594292803970223	32.0	32.0	32.0	32.0	32.0
4	31.21712158808933	32.0	32.0	32.0	32.0	32.0
5	30.89123242349049	32.0	32.0	32.0	32.0	32.0
6	34.30107526881721	36.0	36.0	36.0	32.0	36.0
7	34.572373862696445	36.0	36.0	36.0	32.0	36.0
8	34.34656741108354	36.0	36.0	36.0	32.0	36.0
9	34.59636062861869	36.0	36.0	36.0	32.0	36.0
10	34.07320099255583	36.0	36.0	36.0	32.0	36.0
11	34.60918114143921	36.0	36.0	36.0	32.0	36.0
12	34.50578990901572	36.0	36.0	36.0	32.0	36.0
13	34.593052109181144	36.0	36.0	36.0	32.0	36.0
14	34.466914805624484	36.0	36.0	36.0	32.0	36.0
15	34.162117452440036	36.0	36.0	36.0	32.0	36.0
16	34.41604631927213	36.0	36.0	36.0	32.0	36.0
17	34.34036393713813	36.0	36.0	36.0	32.0	36.0
18	34.44582299421009	36.0	36.0	36.0	32.0	36.0
19	34.21670802315963	36.0	36.0	36.0	32.0	36.0
20	34.33622828784119	36.0	36.0	36.0	32.0	36.0
21	34.48263027295285	36.0	36.0	36.0	32.0	36.0
22	34.295698924731184	36.0	36.0	36.0	32.0	36.0
23	34.344086021505376	36.0	36.0	36.0	32.0	36.0
24	34.391232423490486	36.0	36.0	36.0	32.0	36.0
25	34.19065343258892	36.0	36.0	36.0	32.0	36.0
26	34.229942100909845	36.0	36.0	36.0	32.0	36.0
27	34.11373035566584	36.0	36.0	36.0	32.0	36.0
28	34.08974358974359	36.0	36.0	36.0	32.0	36.0
29	34.02564102564103	36.0	36.0	36.0	32.0	36.0
30	34.05293631100083	36.0	36.0	36.0	32.0	36.0
31	33.844913151364764	36.0	36.0	36.0	32.0	36.0
32	33.953267162944584	36.0	36.0	36.0	32.0	36.0
33	33.782051282051285	36.0	36.0	36.0	32.0	36.0
34	34.08850289495451	36.0	36.0	36.0	32.0	36.0
35	33.74028122415219	36.0	36.0	36.0	27.0	36.0
36	33.7605459057072	36.0	36.0	36.0	32.0	36.0
37	33.715053763440864	36.0	36.0	36.0	32.0	36.0
38	33.644334160463195	36.0	36.0	36.0	27.0	36.0
39	33.869727047146405	36.0	36.0	36.0	32.0	36.0
40	33.66170388751034	36.0	36.0	36.0	27.0	36.0
41	33.75103391232423	36.0	36.0	36.0	32.0	36.0
42	33.85277088502895	36.0	36.0	36.0	32.0	36.0
43	33.61703887510339	36.0	36.0	36.0	27.0	36.0
44	33.2328370554177	36.0	36.0	36.0	21.0	36.0
45	33.15839536807279	36.0	36.0	36.0	14.0	36.0
46	33.35442514474772	36.0	36.0	36.0	21.0	36.0
47	33.59636062861869	36.0	36.0	36.0	27.0	36.0
48	33.42803970223325	36.0	36.0	36.0	21.0	36.0
49	33.279156327543426	36.0	36.0	36.0	21.0	36.0
50	32.394127377998345	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	1.0
24	5.0
25	4.0
26	18.0
27	28.0
28	37.0
29	63.0
30	116.0
31	170.0
32	238.0
33	359.0
34	551.0
35	827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65343258891646	18.817204301075268	13.358147229114973	27.1712158808933
2	17.907361455748553	21.21588089330025	36.72456575682382	24.152191894127377
3	20.6368899917287	23.77998345740281	27.83291976840364	27.750206782464847
4	14.888337468982629	16.253101736972706	35.318444995864354	33.54011579818032
5	17.28701406120761	34.69809760132341	32.25806451612903	15.75682382133995
6	33.62282878411911	36.47642679900744	16.21174524400331	13.688999172870139
7	29.735318444995862	29.776674937965257	20.099255583126553	20.388751033912325
8	27.874276261373037	35.235732009925556	17.866004962779154	19.02398676592225
9	28.949545078577337	11.579818031430934	17.411083540115797	42.05955334987593
10	16.418527708850288	25.806451612903224	31.141439205955336	26.63358147229115
11	39.33002481389578	18.36228287841191	23.61455748552523	18.693134822167078
12	24.317617866004962	23.200992555831267	30.272952853598017	22.208436724565754
13	30.52109181141439	17.783291976840367	25.806451612903224	25.88916459884202
14	21.794871794871796	19.768403639371382	26.96443341604632	31.4722911497105
15	24.11083540115798	29.56989247311828	22.95285359801489	23.366418527708852
16	26.095947063689	24.069478908188586	24.483043837882548	25.351530190239867
17	23.407775020678244	27.08850289495451	24.524400330851943	24.9793217535153
18	24.11083540115798	24.81389578163772	26.344086021505376	24.731182795698924
19	25.351530190239867	24.731182795698924	24.85525227460711	25.06203473945409
20	26.344086021505376	25.31017369727047	24.193548387096776	24.152191894127377
21	26.716294458229946	24.276261373035567	23.283705541770058	25.723738626964433
22	24.68982630272953	24.89660876757651	25.268817204301076	25.144747725392886
23	24.441687344913152	24.028122415219187	25.682382133995034	25.847808105872623
24	24.152191894127377	24.731182795698924	25.930521091811414	25.18610421836228
25	24.069478908188586	23.9454094292804	26.302729528535977	25.682382133995034
26	25.06203473945409	26.01323407775021	25.10339123242349	23.82133995037221
27	25.144747725392886	24.648469809760133	24.937965260545905	25.268817204301076
28	24.317617866004962	26.592224979321756	24.483043837882548	24.607113316790734
29	23.03556658395368	25.806451612903224	24.193548387096776	26.96443341604632
30	23.118279569892472	26.178660049627787	25.847808105872623	24.85525227460711
31	26.38544251447477	25.392886683209266	24.152191894127377	24.069478908188586
32	24.358974358974358	27.29528535980149	22.58064516129032	25.76509511993383
33	23.61455748552523	25.806451612903224	25.516956162117456	25.06203473945409
34	24.400330851943757	26.674937965260547	24.772539288668323	24.152191894127377
35	23.82133995037221	26.592224979321756	24.772539288668323	24.81389578163772
36	23.61455748552523	27.129859387923904	25.599669148056243	23.655913978494624
37	25.268817204301076	26.38544251447477	22.58064516129032	25.76509511993383
38	22.332506203473944	27.83291976840364	24.358974358974358	25.475599669148057
39	25.020678246484696	26.302729528535977	24.400330851943757	24.276261373035567
40	25.43424317617866	25.516956162117456	25.144747725392886	23.904052936311
41	24.56575682382134	25.06203473945409	26.01323407775021	24.358974358974358
42	24.68982630272953	26.344086021505376	24.937965260545905	24.028122415219187
43	25.144747725392886	25.55831265508685	25.020678246484696	24.276261373035567
44	24.069478908188586	27.253928866832094	24.68982630272953	23.98676592224979
45	23.283705541770058	27.129859387923904	24.524400330851943	25.06203473945409
46	25.351530190239867	26.220016542597186	23.82133995037221	24.607113316790734
47	24.9793217535153	25.88916459884202	24.85525227460711	24.276261373035567
48	23.77998345740281	26.26137303556658	24.069478908188586	25.88916459884202
49	24.607113316790734	25.930521091811414	25.020678246484696	24.441687344913152
50	23.9454094292804	27.46071133167907	24.028122415219187	24.56575682382134
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	2.0
8	1.0
9	1.0
10	1.0
11	2.5
12	4.0
13	4.0
14	4.0
15	3.0
16	2.0
17	4.0
18	6.0
19	6.5
20	7.0
21	10.5
22	14.0
23	13.5
24	13.0
25	17.5
26	22.0
27	21.5
28	21.0
29	30.5
30	40.0
31	46.0
32	52.0
33	61.0
34	70.0
35	86.5
36	103.0
37	96.5
38	90.0
39	100.0
40	110.0
41	116.0
42	122.0
43	121.5
44	121.0
45	135.0
46	149.0
47	147.0
48	145.0
49	154.5
50	164.0
51	162.0
52	160.0
53	153.5
54	147.0
55	160.5
56	174.0
57	156.0
58	138.0
59	130.5
60	123.0
61	116.5
62	110.0
63	99.5
64	89.0
65	79.5
66	70.0
67	57.0
68	44.0
69	36.0
70	28.0
71	23.0
72	18.0
73	18.0
74	18.0
75	14.0
76	10.0
77	10.0
78	10.0
79	7.0
80	4.0
81	4.0
82	4.0
83	3.5
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2418.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.20264681555004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.61164205039097	91.97684036393714
2	2.2589052997393573	4.301075268817205
3	0.8253692441355343	2.357320099255583
4	0.1737619461337967	0.6617038875103392
5	0.043440486533449174	0.20678246484698098
6	0.08688097306689835	0.49627791563275436
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	6	0.24813895781637718	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	6	0.24813895781637718	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	5	0.20678246484698098	No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAA	4	0.1654259718775848	No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	4	0.1654259718775848	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC	4	0.1654259718775848	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG	4	0.1654259718775848	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC	3	0.12406947890818859	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3	0.12406947890818859	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG	3	0.12406947890818859	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	3	0.12406947890818859	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC	3	0.12406947890818859	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA	3	0.12406947890818859	No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG	3	0.12406947890818859	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3	0.12406947890818859	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	3	0.12406947890818859	No Hit
GGCTACCGTCGAGACCGATCGCTCTCGTGTCCGTGAGCATTACTCCCCAG	3	0.12406947890818859	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	3	0.12406947890818859	No Hit
ACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	3	0.12406947890818859	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	3	0.12406947890818859	No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG	3	0.12406947890818859	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3	0.12406947890818859	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.12406947890818859	No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCT	3	0.12406947890818859	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	3	0.12406947890818859	No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT	3	0.12406947890818859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0413564929693962	0.0
34	0.0	0.0	0.0	0.0413564929693962	0.0
35	0.0	0.0	0.0	0.0827129859387924	0.0
36	0.0	0.0	0.0	0.0827129859387924	0.0
37	0.0	0.0	0.0	0.0827129859387924	0.0
38	0.0	0.0	0.0	0.12406947890818859	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE