FastQCFastQC Report
Thu 2 Feb 2017
SRR4064116_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064116_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6194400
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82680.13347539713289425No Hit
GCGCAAGACGGACCAGAGCGAAAGC71880.1160402944595118No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG70730.11418377889706832No Hit
GGGTAGGCACACGCTGAGCCAGTCA68080.10990572129665505No Hit
GTCTTGCGCCGGTCCAAGAATTTCA66550.10743574841792587No Hit
GAATAGGACCGCGGTTCTATTTTGT62410.10075229239312929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65100.012.7958711
GGTATCA25750.012.652261
GCGTTAT6550.012.4712041
AAGACGG14050.012.1696755
CCGGTTA550.003068299312.09192114
CGATCCG951.0408821E-612.0005210
CGCCAGT9050.011.96843318
CGAACGA5100.011.73656216
CAAGACG14850.011.6411574
CGGTCCA12850.011.60750810
ACCGTCG10850.011.3833498
CGTTATT6800.011.1727132
TCGGCGT8950.011.03967413
ACTCTAA9150.011.00594210
ACGGTAT10450.011.0006559
ATCGCCA9850.010.99611216
CGCATCG9700.010.96963713
ATCCCGA4600.010.94683516
CGAGCCG14200.010.90610815
CGACCAT11650.010.84596410
Produced by FastQC (version 0.11.5)