##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064116_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6194400
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303193529639675	32.0	32.0	32.0	32.0	32.0
2	30.883038066640836	32.0	32.0	32.0	32.0	32.0
3	30.907639157949117	32.0	32.0	32.0	32.0	32.0
4	30.943163018209997	32.0	32.0	32.0	32.0	32.0
5	30.829339564768176	32.0	32.0	32.0	32.0	32.0
6	34.504235922768956	36.0	36.0	36.0	32.0	36.0
7	34.457335819449824	36.0	36.0	36.0	32.0	36.0
8	34.41498579362004	36.0	36.0	36.0	32.0	36.0
9	34.574213967454476	36.0	36.0	36.0	32.0	36.0
10	34.25253277153558	36.0	36.0	36.0	32.0	36.0
11	34.5349428516079	36.0	36.0	36.0	32.0	36.0
12	34.346297462223944	36.0	36.0	36.0	32.0	36.0
13	34.437208607774764	36.0	36.0	36.0	32.0	36.0
14	34.326896713160274	36.0	36.0	36.0	32.0	36.0
15	34.25349428516079	36.0	36.0	36.0	32.0	36.0
16	34.25674867622369	36.0	36.0	36.0	32.0	36.0
17	34.17853286839726	36.0	36.0	36.0	32.0	36.0
18	34.17515465581816	36.0	36.0	36.0	32.0	36.0
19	34.19944917990443	36.0	36.0	36.0	32.0	36.0
20	34.1862351478755	36.0	36.0	36.0	32.0	36.0
21	34.16899312282061	36.0	36.0	36.0	32.0	36.0
22	34.14727495802661	36.0	36.0	36.0	32.0	36.0
23	34.08281157174222	36.0	36.0	36.0	32.0	36.0
24	34.06773149941883	36.0	36.0	36.0	32.0	36.0
25	33.663547236213354	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	17.0
4	64.0
5	70.0
6	243.0
7	85.0
8	345.0
9	394.0
10	529.0
11	186.0
12	382.0
13	310.0
14	473.0
15	830.0
16	1546.0
17	2062.0
18	2654.0
19	3701.0
20	5577.0
21	8266.0
22	13099.0
23	20488.0
24	30792.0
25	45398.0
26	64773.0
27	86598.0
28	119620.0
29	162392.0
30	214249.0
31	293672.0
32	418325.0
33	596663.0
34	1287574.0
35	2813021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.93643365207324	16.731963423049823	11.055352667891583	26.276250256985357
2	17.244978961386884	19.006239337613437	36.74036221964593	27.00841948135375
3	19.10649280538664	22.719031262193695	28.148711869501685	30.025764062917975
4	12.685768143017617	15.121858606497165	35.29400178813996	36.89837146234525
5	15.087859052242381	35.909074837407026	33.39522277049088	15.60784333985971
6	35.33119218595303	34.8985172349878	16.3953420034087	13.374948575650466
7	31.32964152181864	29.935112066313742	20.05470705968124	18.680539352186383
8	28.805946431472535	32.37794599608735	18.819250226655456	19.99685734578466
9	27.28948085465704	14.097880768795298	18.085541616213202	40.52709676033446
10	15.88769422218087	26.26840458689989	31.13617068291775	26.707730508001486
11	37.7894207893389	20.753044445438714	22.091158028818796	19.36637673640359
12	25.20927362425409	23.178099850414995	28.151445934550907	23.461180590780007
13	29.5414990008707	19.004957684410837	25.025386122683706	26.428157192034753
14	23.970245284006428	19.23803255849719	24.550063875033683	32.2416582824627
15	25.601302213695437	26.777342554277883	21.6104701754522	26.01088505657448
16	26.464560308417763	25.546818775488443	22.869406273196553	25.11921464289724
17	24.57845809102168	25.679281653139856	24.484039940250838	25.25822031558762
18	25.475755724174608	24.685077902929837	25.201118775189325	24.638047597706226
19	25.9669936412895	24.6067346206495	24.77651456587208	24.64975717218892
20	26.122704582361184	24.132334192483306	24.16934878392284	25.57561244123267
21	27.248788885632614	24.01315141118694	23.883323957624164	24.854735745556287
22	26.447145550155067	23.8458153110623	24.388292939535	25.318746199247638
23	24.886306994945272	23.806564764256766	24.935408219567623	26.371720021230345
24	25.18629205818978	24.6569532905875	24.569564894971556	25.58718975625116
25	25.34960411186621	24.083443883440683	24.726759071530967	25.840192933162143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	186.0
1	186.0
2	552.0
3	918.0
4	918.0
5	918.0
6	2498.0
7	4078.0
8	4078.0
9	4078.0
10	5352.5
11	6627.0
12	6627.0
13	6627.0
14	9689.0
15	12751.0
16	12751.0
17	12751.0
18	22314.0
19	31877.0
20	31877.0
21	31877.0
22	51671.0
23	71465.0
24	71465.0
25	71465.0
26	108678.5
27	145892.0
28	145892.0
29	145892.0
30	185120.5
31	224349.0
32	224349.0
33	224349.0
34	291133.0
35	357917.0
36	357917.0
37	357917.0
38	418795.0
39	479673.0
40	479673.0
41	479673.0
42	558628.0
43	637583.0
44	637583.0
45	637583.0
46	714825.0
47	792067.0
48	792067.0
49	792067.0
50	822031.5
51	851996.0
52	851996.0
53	851996.0
54	806128.0
55	760260.0
56	760260.0
57	760260.0
58	704802.0
59	649344.0
60	649344.0
61	649344.0
62	574981.0
63	500618.0
64	500618.0
65	500618.0
66	412327.5
67	324037.0
68	324037.0
69	324037.0
70	244016.5
71	163996.0
72	163996.0
73	163996.0
74	125469.5
75	86943.0
76	86943.0
77	86943.0
78	69693.0
79	52443.0
80	52443.0
81	52443.0
82	35769.0
83	19095.0
84	19095.0
85	19095.0
86	14871.0
87	10647.0
88	10647.0
89	10647.0
90	7564.0
91	4481.0
92	4481.0
93	4481.0
94	3121.0
95	1761.0
96	1761.0
97	1761.0
98	2578.5
99	3396.0
100	3396.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03895453958414052
2	0.005052951052563606
3	0.0018242283352705672
4	0.004100477850962159
5	0.009024279994834043
6	0.013496060958284904
7	0.02284321322484825
8	0.03522536484566705
9	0.04925416505230531
10	0.057342115459124376
11	0.05442012139997417
12	0.06360583753067287
13	0.06473589048172543
14	0.06760945370011623
15	0.05990895001937234
16	0.06610809763657498
17	0.06220134314865039
18	0.07706961126178484
19	0.07463192561022859
20	0.07989474363941625
21	0.0750032287227173
22	0.07807051530414569
23	0.08305889190236343
24	0.07734405269275475
25	0.07908756296009299
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	6194400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.65569517600627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.59307444217227	21.739248239068313
2	16.036669534845007	10.794505230061798
3	6.999157077251682	7.066844912429087
4	3.575218784035789	4.813058943321613
5	2.1909478576612194	3.6868936621984982
6	1.3821155697384402	2.7909636187877505
7	0.9671214362559345	2.2784400979767776
8	0.7068419344641541	1.9031405347155337
9	0.5294477232248437	1.6037038066037286
>10	2.6777836489642755	16.174050053093257
>50	0.1916703394069104	4.464417076648907
>100	0.1182485335565188	8.03030893594806
>500	0.01724882284723966	4.094423560711865
>1k	0.01358703784110028	8.799484908988095
>5k	8.672577345291137E-4	1.7605164194467842
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8268	0.13347539713289425	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	7188	0.1160402944595118	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	7073	0.11418377889706832	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	6808	0.10990572129665505	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	6655	0.10743574841792587	No Hit
GAATAGGACCGCGGTTCTATTTTGT	6241	0.10075229239312929	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.8430840759395585E-5
2	0.0	0.0	0.0	0.0	4.8430840759395585E-5
3	0.0	0.0	0.0	0.0	4.8430840759395585E-5
4	0.0	0.0	0.0	0.0	4.8430840759395585E-5
5	0.0	0.0	0.0	0.0	4.8430840759395585E-5
6	0.0	0.0	0.0	0.0	6.457445434586077E-5
7	0.0	0.0	0.0	0.0	6.457445434586077E-5
8	0.0	0.0	0.0	0.0	6.457445434586077E-5
9	0.0	0.0	0.0	0.0	6.457445434586077E-5
10	0.0	0.0	0.0	0.0	6.457445434586077E-5
11	0.0	0.0	0.0	3.228722717293039E-5	6.457445434586077E-5
12	0.0	0.0	0.0	3.228722717293039E-5	1.4529252227818675E-4
13	0.0	0.0	0.0	4.8430840759395585E-5	1.6143613586465193E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6510	0.0	12.795871	1
GGTATCA	2575	0.0	12.65226	1
GCGTTAT	655	0.0	12.471204	1
AAGACGG	1405	0.0	12.169675	5
CCGGTTA	55	0.0030682993	12.091921	14
CGATCCG	95	1.0408821E-6	12.00052	10
CGCCAGT	905	0.0	11.968433	18
CGAACGA	510	0.0	11.736562	16
CAAGACG	1485	0.0	11.641157	4
CGGTCCA	1285	0.0	11.607508	10
ACCGTCG	1085	0.0	11.383349	8
CGTTATT	680	0.0	11.172713	2
TCGGCGT	895	0.0	11.039674	13
ACTCTAA	915	0.0	11.005942	10
ACGGTAT	1045	0.0	11.000655	9
ATCGCCA	985	0.0	10.996112	16
CGCATCG	970	0.0	10.969637	13
ATCCCGA	460	0.0	10.946835	16
CGAGCCG	1420	0.0	10.906108	15
CGACCAT	1165	0.0	10.845964	10
>>END_MODULE