FastQCFastQC Report
Thu 2 Feb 2017
SRR4064116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6194400
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT70670.11408691721554952No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA67020.10819449825648973No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC65180.10522407335658013No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT63220.10205992509363296No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC63080.10183391450342245No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA62430.10078457962030221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71750.026.7677481
GGTATCA34800.023.5802881
TACCGTC16100.018.3092427
ATACCGT16550.017.9441856
TCAACGC105150.017.929144
CGCAATA19750.017.82452436
ATCAACG107950.017.4843333
ACCGTCG16500.017.4655518
CAACGCA107900.017.4518015
ATACGAA20100.017.40285740
AACGCAG108850.017.3399096
TAGCGGC20750.017.28305630
CTAGCGG20850.017.19933129
CGTCGTA17100.017.11084710
CAATACG20850.017.09436638
CGGATCG5100.016.82462926
GCATATA12300.016.8128851
TATCAAC114650.016.6928182
TCTAGCG21750.016.58891928
TACGAAT21350.016.2809141
Produced by FastQC (version 0.11.5)