##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6194400
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.345634928322355	32.0	32.0	32.0	32.0	32.0
2	31.411229174738473	32.0	32.0	32.0	32.0	32.0
3	31.490512075422963	32.0	32.0	32.0	32.0	32.0
4	31.574713773731112	32.0	32.0	32.0	32.0	32.0
5	31.519911532997547	32.0	32.0	32.0	32.0	32.0
6	35.12026992121917	36.0	36.0	36.0	36.0	36.0
7	35.13372998191915	36.0	36.0	36.0	36.0	36.0
8	35.07937524215421	36.0	36.0	36.0	36.0	36.0
9	35.18926885574067	36.0	36.0	36.0	36.0	36.0
10	35.04708301046106	36.0	36.0	36.0	36.0	36.0
11	35.184793846054504	36.0	36.0	36.0	36.0	36.0
12	35.09122126436782	36.0	36.0	36.0	36.0	36.0
13	35.134974815962806	36.0	36.0	36.0	36.0	36.0
14	35.07924367170347	36.0	36.0	36.0	36.0	36.0
15	35.0509587692109	36.0	36.0	36.0	36.0	36.0
16	35.0633885444918	36.0	36.0	36.0	36.0	36.0
17	35.02457623014335	36.0	36.0	36.0	36.0	36.0
18	35.0255482371174	36.0	36.0	36.0	36.0	36.0
19	35.027581040940206	36.0	36.0	36.0	36.0	36.0
20	35.02080023892548	36.0	36.0	36.0	36.0	36.0
21	35.01457542296268	36.0	36.0	36.0	36.0	36.0
22	34.99988279736536	36.0	36.0	36.0	36.0	36.0
23	34.94291069352964	36.0	36.0	36.0	36.0	36.0
24	34.91306631796461	36.0	36.0	36.0	32.0	36.0
25	34.88596361229498	36.0	36.0	36.0	32.0	36.0
26	34.82637139997417	36.0	36.0	36.0	32.0	36.0
27	34.82418297171639	36.0	36.0	36.0	32.0	36.0
28	34.80202876791941	36.0	36.0	36.0	32.0	36.0
29	34.77319740410694	36.0	36.0	36.0	32.0	36.0
30	34.759558310732274	36.0	36.0	36.0	32.0	36.0
31	34.75544346506522	36.0	36.0	36.0	32.0	36.0
32	34.72175690946661	36.0	36.0	36.0	32.0	36.0
33	34.69949841792587	36.0	36.0	36.0	32.0	36.0
34	34.69339661629859	36.0	36.0	36.0	32.0	36.0
35	34.656208672349216	36.0	36.0	36.0	32.0	36.0
36	34.63755956993413	36.0	36.0	36.0	32.0	36.0
37	34.63557342115459	36.0	36.0	36.0	32.0	36.0
38	34.594546203022084	36.0	36.0	36.0	32.0	36.0
39	34.602338402427996	36.0	36.0	36.0	32.0	36.0
40	34.57986455508201	36.0	36.0	36.0	32.0	36.0
41	34.572232338886735	36.0	36.0	36.0	32.0	36.0
42	34.504353609712	36.0	36.0	36.0	32.0	36.0
43	34.49807810280253	36.0	36.0	36.0	32.0	36.0
44	34.443461352189075	36.0	36.0	36.0	32.0	36.0
45	34.424769630634124	36.0	36.0	36.0	32.0	36.0
46	34.41307471264368	36.0	36.0	36.0	32.0	36.0
47	34.39423204830169	36.0	36.0	36.0	32.0	36.0
48	34.3543792780576	36.0	36.0	36.0	32.0	36.0
49	34.343082300142065	36.0	36.0	36.0	32.0	36.0
50	33.735129310344824	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	7.0
18	5.0
19	17.0
20	53.0
21	233.0
22	695.0
23	1877.0
24	5017.0
25	11232.0
26	22429.0
27	40811.0
28	67431.0
29	102287.0
30	148327.0
31	211596.0
32	307518.0
33	499498.0
34	1057930.0
35	3717435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06861945762504	17.586129511920657	11.55745922985145	26.787791800602857
2	16.42888911310665	19.725735041872667	36.26934113611139	27.57603470890929
3	18.34161557463881	23.26606480908979	28.475837283420546	29.91648233285085
4	12.28608097636575	15.757829652589436	35.37049593180937	36.58559343923544
5	14.645954410435232	36.38951310861423	33.15925674803048	15.805275732920057
6	35.396583293323985	35.24076478282109	16.1607623419012	13.201889581953724
7	31.23304314640422	30.117938821496455	20.056186250867317	18.59283178123201
8	28.785919540229887	32.566656980498514	18.631925610228596	20.015497869043006
9	27.33517889447788	13.963449107101633	17.91853894582813	40.78283305259236
10	15.760022368793209	26.511136611076168	31.17469743365883	26.55414358647179
11	37.99556276422064	20.658346567066978	22.02007717619649	19.326013492515898
12	25.195043449805944	23.25076775126651	28.075020797047134	23.479168001880414
13	29.558444342703343	18.99550107742964	25.061991756387897	26.38406282347912
14	23.954638287856408	19.183625433799985	24.54391370768407	32.31782257065953
15	25.62473311403951	26.90024821907856	21.53145117349101	25.943567493390912
16	26.550087216646794	25.574213254185874	22.798052094289226	25.077647434878102
17	24.627147100607	25.754778509621595	24.41929807568126	25.198776314090143
18	25.57414497161542	24.65232625999191	25.136845707846707	24.636683060545963
19	26.03066092449001	24.64560322962728	24.723883624545334	24.599852221337372
20	26.28246284396864	24.02172560127768	24.131067161031076	25.564744393722606
21	27.310829135993803	24.009799173446986	23.809795944724264	24.86957574583495
22	26.491635257885637	23.841189927729804	24.386360374616206	25.28081443976835
23	24.952246790138368	23.751361725237263	24.952166071392824	26.34422541323155
24	25.28705089071176	24.570271207476722	24.594728892614135	25.547949009197378
25	25.465060695326457	24.007252475219552	24.756376790981513	25.77131003847247
26	25.29256389570943	24.87942994941813	24.87516784071079	24.952838314161646
27	25.845523757584154	24.422856890077995	24.267141706951616	25.464477645386236
28	25.25169341424633	24.1996673308963	25.054991465324584	25.49364778953279
29	25.080929973052402	24.432182523000385	25.035872012791234	25.45101549115598
30	24.989942139047102	24.531332739225334	25.215121985870564	25.263603135857
31	25.613244558390942	24.460666393281176	24.201655166178554	25.724433882149327
32	25.533604188952307	24.306003779666067	24.04222467541399	26.118167355967632
33	24.97732124467015	24.160627743427117	24.602982536833707	26.259068475069032
34	25.744742679465695	24.158764138180434	24.9768978176429	25.11959536471097
35	26.260802798992867	23.96789398130001	24.91387142998288	24.85743178972424
36	25.094282079841935	24.597826931053117	24.460660006041415	25.847230983063536
37	26.136634419108052	24.398240020767936	24.41606324921329	25.04906231091072
38	25.306942279013427	24.102599372900862	24.785737361349852	25.80472098673586
39	25.93789003579672	24.180843096454183	24.206835392725033	25.67443147502407
40	26.10294681500431	24.307264466528377	24.553702814886137	25.036085903581178
41	24.9198323012662	24.831669223627316	25.06380562600225	25.184692849104234
42	26.410464087011732	24.625546329676794	24.574660764242427	24.389328819069043
43	25.587983544887837	23.6608265720163	24.77941838843609	25.971771494659773
44	25.084859973375618	24.401201616577247	24.594878987563128	25.919059422484008
45	25.31076877576152	24.551944072598168	24.661237818671182	25.47604933296913
46	25.125606561600893	24.36788281951497	24.758255194821338	25.748255424062798
47	25.299167376327336	24.263953408590996	25.082338126737998	25.354541088343673
48	26.215855988381538	24.84869100159857	24.002942715841943	24.93251029417795
49	25.242385541573498	24.869288500342325	24.36457187635153	25.523754081732648
50	25.37767389013672	24.856016798672457	24.331744399164457	25.434564912026364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	53.0
2	76.0
3	232.5
4	389.0
5	474.0
6	559.0
7	512.5
8	466.0
9	652.5
10	839.0
11	1313.0
12	1787.0
13	3386.5
14	4986.0
15	6500.5
16	8015.0
17	8249.5
18	8484.0
19	8642.0
20	8800.0
21	10687.0
22	12574.0
23	15190.0
24	17806.0
25	23415.0
26	29024.0
27	36914.0
28	44804.0
29	53213.0
30	61622.0
31	69868.0
32	78114.0
33	90829.0
34	103544.0
35	121601.0
36	139658.0
37	170477.0
38	201296.0
39	225124.0
40	248952.0
41	278099.0
42	307246.0
43	320359.5
44	333473.0
45	368361.0
46	403249.0
47	435132.5
48	467016.0
49	483551.5
50	500087.0
51	488198.0
52	476309.0
53	471042.0
54	465775.0
55	471218.0
56	476661.0
57	465357.5
58	454054.0
59	414052.0
60	374050.0
61	329605.5
62	285161.0
63	248619.5
64	212078.0
65	181009.5
66	149941.0
67	128097.5
68	106254.0
69	95681.5
70	85109.0
71	64967.0
72	44825.0
73	38682.5
74	32540.0
75	24686.0
76	16832.0
77	14730.0
78	12628.0
79	10420.0
80	8212.0
81	6669.0
82	5126.0
83	4121.0
84	3116.0
85	2204.5
86	1293.0
87	931.0
88	569.0
89	434.0
90	299.0
91	264.0
92	229.0
93	179.5
94	130.0
95	138.5
96	147.0
97	102.5
98	58.0
99	83.0
100	108.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008556115200826553
2	4.035903396616298E-4
3	3.5515949890223427E-4
4	0.0
5	0.0
6	2.2601059021051272E-4
7	3.228722717293039E-5
8	0.0
9	0.001259201859744285
10	9.040423608420509E-4
11	4.8430840759395585E-5
12	1.7757974945111713E-4
13	4.5202118042102544E-4
14	2.0986697662404754E-4
15	0.0044394937362779285
16	8.878987472555857E-4
17	0.0
18	2.421542037969779E-4
19	1.6143613586465194E-5
20	7.910370657367944E-4
21	0.0
22	1.1300529510525636E-4
23	8.394679064961902E-4
24	4.5202118042102544E-4
25	0.0016305049722329847
26	0.00456864264496965
27	0.006441301820999613
28	0.004148908691721555
29	0.00251840371948857
30	0.0038744672607516463
31	0.007426062249773989
32	0.0032610099444659695
33	0.004471780963450859
34	0.002905850445563735
35	0.0027767015368720133
36	0.00786193981660855
37	0.003809892806405786
38	0.007797365362262688
39	0.004148908691721555
40	0.002469972878729175
41	0.0031802918765336434
42	0.0017596538809247061
43	0.0015174996771277283
44	0.001259201859744285
45	0.0013560635412630763
46	9.363295880149813E-4
47	0.002017951698308149
48	0.0019695208575487536
49	5.327392483533514E-4
50	0.0016305049722329847
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	6194400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.809874178254795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.4244054967041	23.428397290240948
2	15.880954920293302	11.37389995052479
3	6.833403546399812	7.341099636174519
4	3.5873664254783817	5.13852561310706
5	2.117322458666535	3.7910525419820846
6	1.3623528687310607	2.927141088938625
7	0.9077173426796812	2.275367068154542
8	0.6512504643589904	1.865695774978036
9	0.4630141975757939	1.4922432142141306
>10	2.472319347848527	15.83813161649099
>50	0.16852257317277225	4.151063941215233
>100	0.10514913457713806	7.607362861888116
>500	0.014063301678245176	3.573547039550335
>1k	0.011613537275810456	8.022019447880298
>5k	5.443845597901439E-4	1.1744529146603868
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	7067	0.11408691721554952	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	6702	0.10819449825648973	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	6518	0.10522407335658013	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	6322	0.10205992509363296	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	6308	0.10183391450342245	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	6243	0.10078457962030221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.6143613586465194E-5
7	0.0	0.0	0.0	1.6143613586465194E-5	1.6143613586465194E-5
8	0.0	0.0	0.0	1.6143613586465194E-5	1.6143613586465194E-5
9	0.0	0.0	0.0	1.6143613586465194E-5	1.6143613586465194E-5
10	0.0	0.0	0.0	1.6143613586465194E-5	1.6143613586465194E-5
11	0.0	0.0	0.0	3.228722717293039E-5	1.6143613586465194E-5
12	1.6143613586465194E-5	0.0	0.0	3.228722717293039E-5	1.4529252227818675E-4
13	1.6143613586465194E-5	0.0	0.0	4.8430840759395585E-5	1.4529252227818675E-4
14	1.6143613586465194E-5	0.0	0.0	4.8430840759395585E-5	1.4529252227818675E-4
15	1.6143613586465194E-5	0.0	0.0	9.686168151879117E-5	1.7757974945111713E-4
16	1.6143613586465194E-5	0.0	0.0	9.686168151879117E-5	1.7757974945111713E-4
17	1.6143613586465194E-5	0.0	0.0	9.686168151879117E-5	1.9372336303758234E-4
18	1.6143613586465194E-5	0.0	0.0	1.1300529510525636E-4	2.0986697662404752E-4
19	1.6143613586465194E-5	0.0	0.0	1.6143613586465193E-4	2.0986697662404752E-4
20	1.6143613586465194E-5	0.0	1.6143613586465194E-5	1.7757974945111713E-4	2.2601059021051272E-4
21	1.6143613586465194E-5	0.0	1.6143613586465194E-5	1.7757974945111713E-4	2.582978173834431E-4
22	1.6143613586465194E-5	0.0	1.6143613586465194E-5	2.4215420379697792E-4	2.744414309699083E-4
23	1.6143613586465194E-5	0.0	1.6143613586465194E-5	3.7130311248869947E-4	2.744414309699083E-4
24	1.6143613586465194E-5	0.0	1.6143613586465194E-5	5.81170089112747E-4	2.744414309699083E-4
25	1.6143613586465194E-5	0.0	1.6143613586465194E-5	8.556115200826553E-4	2.744414309699083E-4
26	1.6143613586465194E-5	0.0	1.6143613586465194E-5	0.0010654784967067028	3.067286581428387E-4
27	1.6143613586465194E-5	0.0	1.6143613586465194E-5	0.0015982177450600543	3.2287227172930386E-4
28	1.6143613586465194E-5	0.0	1.6143613586465194E-5	0.002841275991217874	3.2287227172930386E-4
29	1.6143613586465194E-5	0.0	1.6143613586465194E-5	0.005795557277541005	3.3901588531576906E-4
30	1.6143613586465194E-5	0.0	1.6143613586465194E-5	0.011445822032803823	3.3901588531576906E-4
31	1.6143613586465194E-5	0.0	1.6143613586465194E-5	0.027524861164923156	3.5515949890223427E-4
32	1.6143613586465194E-5	0.0	1.6143613586465194E-5	0.0435070386155237	3.5515949890223427E-4
33	1.6143613586465194E-5	0.0	1.6143613586465194E-5	0.06111972103835723	3.7130311248869947E-4
34	3.228722717293039E-5	0.0	1.6143613586465194E-5	0.08129923802143872	3.7130311248869947E-4
35	3.228722717293039E-5	0.0	1.6143613586465194E-5	0.10472362133539971	3.7130311248869947E-4
36	3.228722717293039E-5	0.0	1.6143613586465194E-5	0.1402072839984502	3.7130311248869947E-4
37	3.228722717293039E-5	0.0	1.6143613586465194E-5	0.19340049076585303	3.7130311248869947E-4
38	4.8430840759395585E-5	0.0	1.6143613586465194E-5	0.27418313315252485	3.7130311248869947E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7175	0.0	26.767748	1
GGTATCA	3480	0.0	23.580288	1
TACCGTC	1610	0.0	18.309242	7
ATACCGT	1655	0.0	17.944185	6
TCAACGC	10515	0.0	17.92914	4
CGCAATA	1975	0.0	17.824524	36
ATCAACG	10795	0.0	17.484333	3
ACCGTCG	1650	0.0	17.465551	8
CAACGCA	10790	0.0	17.451801	5
ATACGAA	2010	0.0	17.402857	40
AACGCAG	10885	0.0	17.339909	6
TAGCGGC	2075	0.0	17.283056	30
CTAGCGG	2085	0.0	17.199331	29
CGTCGTA	1710	0.0	17.110847	10
CAATACG	2085	0.0	17.094366	38
CGGATCG	510	0.0	16.824629	26
GCATATA	1230	0.0	16.812885	1
TATCAAC	11465	0.0	16.692818	2
TCTAGCG	2175	0.0	16.588919	28
TACGAAT	2135	0.0	16.28091	41
>>END_MODULE