FastQCFastQC Report
Thu 2 Feb 2017
SRR4064115_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064115_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501717
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50300.33494992731653167No Hit
TATCAACGCAGAGTACTTTTTTTTT34290.22833862838337718No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30880.20563128738637174No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20990.13977333945077536No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19170.12765387885999827No Hit
GGTATCAACGCAGAGTACTTTTTTT17140.11413601897028533No Hit
GTACATGGGGTGGTATCAACGCAAA15940.10614516583350925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC707.2824314E-613.5677643
TCGCACG1500.013.3021713
CCGATCA500.001499168513.3003979
GTTATTC1102.757588E-912.9510483
AACGGTA550.003066144712.09207518
CGTTATT951.0451786E-611.9947622
TTCGGGC951.0451786E-611.9947622
GTATAGG1052.7254282E-711.7599041
TAGACTG1157.078597E-811.5644065
ACGCCCC1657.2759576E-1211.51587917
CGCCGGT2400.011.4795097
TGGACCG1501.7644197E-1011.39925
CGACCAT1501.7644197E-1011.399210
GATATAC2000.011.3980611
TGTAGGA3500.011.3950232
CCGACCA1701.0913936E-1111.1768049
CGCACGC1803.6379788E-1211.08514114
CGAGCCG2850.011.00216115
CTATAAG1303.2727257E-810.9596741
GTGTATA2950.010.9472891
Produced by FastQC (version 0.11.5)