##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064115_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501717
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.196208739729258	32.0	32.0	32.0	32.0	32.0
2	30.84407315093323	32.0	32.0	32.0	32.0	32.0
3	30.87324442621346	32.0	32.0	32.0	32.0	32.0
4	30.891334385906266	32.0	32.0	32.0	32.0	32.0
5	30.820118570942462	32.0	32.0	32.0	32.0	32.0
6	34.455414701971144	36.0	36.0	36.0	32.0	36.0
7	34.38736726027607	36.0	36.0	36.0	32.0	36.0
8	34.3383373831421	36.0	36.0	36.0	32.0	36.0
9	34.44884688659714	36.0	36.0	36.0	32.0	36.0
10	34.19851276905036	36.0	36.0	36.0	32.0	36.0
11	34.45016337965143	36.0	36.0	36.0	32.0	36.0
12	34.27806437564468	36.0	36.0	36.0	32.0	36.0
13	34.35879663078995	36.0	36.0	36.0	32.0	36.0
14	34.26483152285018	36.0	36.0	36.0	32.0	36.0
15	34.21320262073347	36.0	36.0	36.0	32.0	36.0
16	34.218321428072	36.0	36.0	36.0	32.0	36.0
17	34.139301213211276	36.0	36.0	36.0	32.0	36.0
18	34.14041260770172	36.0	36.0	36.0	32.0	36.0
19	34.145474813163865	36.0	36.0	36.0	32.0	36.0
20	34.115974581096175	36.0	36.0	36.0	32.0	36.0
21	34.09559590788411	36.0	36.0	36.0	32.0	36.0
22	34.0864417197115	36.0	36.0	36.0	32.0	36.0
23	34.05037633588752	36.0	36.0	36.0	32.0	36.0
24	34.03518905359665	36.0	36.0	36.0	32.0	36.0
25	33.65008253885386	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	12.0
5	15.0
6	57.0
7	20.0
8	85.0
9	111.0
10	142.0
11	44.0
12	79.0
13	92.0
14	150.0
15	289.0
16	470.0
17	561.0
18	825.0
19	1103.0
20	1709.0
21	2438.0
22	3862.0
23	5817.0
24	8412.0
25	12061.0
26	16879.0
27	22480.0
28	30085.0
29	40441.0
30	52485.0
31	71402.0
32	99859.0
33	141230.0
34	304115.0
35	684381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.241051741021764	17.52959437223958	11.604591207956673	25.62476267878198
2	16.074889087487364	20.260462935708702	38.56818638896029	25.096461587843642
3	18.450309084479986	24.992591694840424	28.965640780106188	27.59145844057341
4	12.26108266130375	16.04819967103292	36.90877489728103	34.78194277038231
5	14.0130395982898	37.16445344237403	33.975479161949416	14.847027797386753
6	32.41679420426528	36.49080463903863	17.77114893061555	13.32125222608054
7	29.265154098640178	31.116589048979073	21.415621403299372	18.202635449081374
8	27.48728156161173	33.121764600474165	19.975525707666105	19.415428130248
9	27.682898392372934	14.312078189437496	18.832754818550672	39.1722685996389
10	15.4150036979538	26.85100910829341	32.341371107986916	25.39261608576588
11	35.950429742154704	21.512625757878606	23.655806516090347	18.881137983876343
12	24.917809296245014	23.79485840010981	29.58139045215215	21.705941851493026
13	29.033758386623248	20.21232008198551	25.790786358581325	24.963135172809917
14	23.394513752933634	20.10471127435367	26.208670898475244	30.292104074237454
15	24.50966935496767	27.61596296434492	23.265819309925746	24.608548370761664
16	24.70585726689309	26.21693497175017	24.725980981091038	24.3512267802657
17	23.14387813779129	26.242406433467462	25.98900436240694	24.624711066334307
18	23.634693957793118	25.57076986540367	27.29372548000381	23.500810696799395
19	24.94030780713241	25.14382436534354	26.407372977079397	23.50849485044465
20	25.3949678174371	24.701733153304694	25.945383611617977	23.95791541764023
21	26.381619545326718	24.35455559598399	25.212415883310207	24.051408975379083
22	25.28934410005938	24.745676415765164	25.84347462162182	24.12150486255364
23	23.973432165181237	24.923806750058816	26.08051792082567	25.02224316393428
24	24.18681118025502	25.36049538376648	26.223165268757153	24.229528167221343
25	24.278083775669668	25.036803049538335	26.206970087734206	24.478143087057788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	204.0
1	204.0
2	558.5
3	913.0
4	913.0
5	913.0
6	2428.5
7	3944.0
8	3944.0
9	3944.0
10	4005.0
11	4066.0
12	4066.0
13	4066.0
14	4361.5
15	4657.0
16	4657.0
17	4657.0
18	8197.5
19	11738.0
20	11738.0
21	11738.0
22	18998.5
23	26259.0
24	26259.0
25	26259.0
26	38795.0
27	51331.0
28	51331.0
29	51331.0
30	63605.0
31	75879.0
32	75879.0
33	75879.0
34	90358.0
35	104837.0
36	104837.0
37	104837.0
38	118386.0
39	131935.0
40	131935.0
41	131935.0
42	148237.0
43	164539.0
44	164539.0
45	164539.0
46	180667.5
47	196796.0
48	196796.0
49	196796.0
50	199998.5
51	203201.0
52	203201.0
53	203201.0
54	184746.5
55	166292.0
56	166292.0
57	166292.0
58	149807.5
59	133323.0
60	133323.0
61	133323.0
62	115162.5
63	97002.0
64	97002.0
65	97002.0
66	79083.0
67	61164.0
68	61164.0
69	61164.0
70	46092.0
71	31020.0
72	31020.0
73	31020.0
74	23374.5
75	15729.0
76	15729.0
77	15729.0
78	12589.5
79	9450.0
80	9450.0
81	9450.0
82	6525.0
83	3600.0
84	3600.0
85	3600.0
86	2746.5
87	1893.0
88	1893.0
89	1893.0
90	1346.0
91	799.0
92	799.0
93	799.0
94	577.5
95	356.0
96	356.0
97	356.0
98	573.0
99	790.0
100	790.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039088589927396444
2	0.005527006752936805
3	0.0015981706273552206
4	0.003129750811903974
5	0.009122890664486053
6	0.013251498118487038
7	0.022707340997005426
8	0.036624743543557145
9	0.04974306077643125
10	0.05906572276933669
11	0.05440439177288397
12	0.06252842579527301
13	0.0641931868654347
14	0.0678556612197904
15	0.0594652654261755
16	0.06659044280646753
17	0.06259501623807948
18	0.07697855188427646
19	0.07338266797272722
20	0.08090738800985806
21	0.07478106727166303
22	0.07777763719795408
23	0.08363759616492321
24	0.07611287612779238
25	0.07737809454111527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1501717.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.64240743952432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.11103794614412	38.27272417380822
2	14.685180759662812	15.167561762271403
3	4.967259706475438	7.695637488592095
4	2.195284431036164	4.534790921328557
5	1.1085837182589542	2.8624966029575876
6	0.6421540056225616	1.989742727836975
7	0.42999355052931704	1.5544131492961875
8	0.3078489538219517	1.2718448882483628
9	0.2242685901626806	1.0423592918161961
>10	1.1670879249614217	10.757402329335875
>50	0.07896314694934121	2.8196110435188038
>100	0.0728718132454573	7.539304890234838
>500	0.00752049700723172	2.6210061596959795
>1k	0.0018152923810559323	1.534288338284042
>5k	1.2966374150399516E-4	0.3368162327747886
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5030	0.33494992731653167	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3429	0.22833862838337718	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3088	0.20563128738637174	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2099	0.13977333945077536	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1917	0.12765387885999827	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1714	0.11413601897028533	No Hit
GTACATGGGGTGGTATCAACGCAAA	1594	0.10614516583350925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.659044280646753E-5
2	0.0	0.0	0.0	0.0	1.3318088561293506E-4
3	0.0	0.0	0.0	6.659044280646753E-5	1.3318088561293506E-4
4	0.0	0.0	0.0	6.659044280646753E-5	1.3318088561293506E-4
5	0.0	0.0	0.0	6.659044280646753E-5	1.3318088561293506E-4
6	0.0	0.0	0.0	6.659044280646753E-5	1.3318088561293506E-4
7	0.0	0.0	0.0	6.659044280646753E-5	1.3318088561293506E-4
8	0.0	0.0	0.0	6.659044280646753E-5	1.3318088561293506E-4
9	0.0	0.0	0.0	1.3318088561293506E-4	1.3318088561293506E-4
10	0.0	0.0	0.0	1.3318088561293506E-4	1.3318088561293506E-4
11	0.0	0.0	0.0	1.3318088561293506E-4	1.3318088561293506E-4
12	0.0	0.0	0.0	1.3318088561293506E-4	5.993139852582078E-4
13	0.0	0.0	0.0	1.3318088561293506E-4	7.324948708711429E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	70	7.2824314E-6	13.567764	3
TCGCACG	150	0.0	13.30217	13
CCGATCA	50	0.0014991685	13.300397	9
GTTATTC	110	2.757588E-9	12.951048	3
AACGGTA	55	0.0030661447	12.092075	18
CGTTATT	95	1.0451786E-6	11.994762	2
TTCGGGC	95	1.0451786E-6	11.994762	2
GTATAGG	105	2.7254282E-7	11.759904	1
TAGACTG	115	7.078597E-8	11.564406	5
ACGCCCC	165	7.2759576E-12	11.515879	17
CGCCGGT	240	0.0	11.479509	7
TGGACCG	150	1.7644197E-10	11.3992	5
CGACCAT	150	1.7644197E-10	11.3992	10
GATATAC	200	0.0	11.398061	1
TGTAGGA	350	0.0	11.395023	2
CCGACCA	170	1.0913936E-11	11.176804	9
CGCACGC	180	3.6379788E-12	11.085141	14
CGAGCCG	285	0.0	11.002161	15
CTATAAG	130	3.2727257E-8	10.959674	1
GTGTATA	295	0.0	10.947289	1
>>END_MODULE