FastQCFastQC Report
Thu 2 Feb 2017
SRR4064115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501717
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15380.10241610103634707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG4650.020.3428554
ACTAGAC1605.2750693E-1119.2481943
GTATCAA37400.019.116491
CTAGCCG600.00741265718.3334459
CGCACGA600.00741408118.33283410
CCGTCGT3150.018.1588429
CGTCGTA3200.017.87451410
GTATAGA1852.1827873E-1117.836761
ATACGAT750.001291592117.5989346
ATACCGT3500.017.5989346
ACCGTCG3400.017.469538
AACGCAG42650.016.8149266
TACCGTC3700.016.6476427
ATACTAG1351.0865606E-616.295311
TACGCTA3450.015.9421259
GTCGTAG3600.015.88792711
TCAATAC1805.2750693E-915.8873993
CTTACGC850.002982254415.5279543
ACGTATG1004.9419556E-415.40111832
CTATACA2001.2787496E-915.3990684
Produced by FastQC (version 0.11.5)