##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1501717
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2932410034647	32.0	32.0	32.0	32.0	32.0
2	31.392974175560376	32.0	32.0	32.0	32.0	32.0
3	31.46735836379291	32.0	32.0	32.0	32.0	32.0
4	31.552342418711383	32.0	32.0	32.0	32.0	32.0
5	31.499402350775814	32.0	32.0	32.0	32.0	32.0
6	35.0782937131297	36.0	36.0	36.0	36.0	36.0
7	35.09482678826969	36.0	36.0	36.0	36.0	36.0
8	35.043733273313144	36.0	36.0	36.0	36.0	36.0
9	35.152256383859275	36.0	36.0	36.0	36.0	36.0
10	35.01183445349557	36.0	36.0	36.0	36.0	36.0
11	35.16090115514441	36.0	36.0	36.0	36.0	36.0
12	35.06936593246264	36.0	36.0	36.0	36.0	36.0
13	35.10713137029148	36.0	36.0	36.0	36.0	36.0
14	35.05119872785618	36.0	36.0	36.0	36.0	36.0
15	35.02553743481628	36.0	36.0	36.0	36.0	36.0
16	35.04176419391936	36.0	36.0	36.0	36.0	36.0
17	35.00194777045209	36.0	36.0	36.0	36.0	36.0
18	35.00211824198568	36.0	36.0	36.0	36.0	36.0
19	34.99158097031598	36.0	36.0	36.0	36.0	36.0
20	34.983940382908365	36.0	36.0	36.0	36.0	36.0
21	34.9828236611825	36.0	36.0	36.0	36.0	36.0
22	34.967940031310825	36.0	36.0	36.0	36.0	36.0
23	34.918776307386814	36.0	36.0	36.0	32.0	36.0
24	34.908404845919705	36.0	36.0	36.0	32.0	36.0
25	34.87070999396025	36.0	36.0	36.0	32.0	36.0
26	34.81402487952124	36.0	36.0	36.0	32.0	36.0
27	34.813941641467736	36.0	36.0	36.0	32.0	36.0
28	34.788355595628204	36.0	36.0	36.0	32.0	36.0
29	34.75846913899223	36.0	36.0	36.0	32.0	36.0
30	34.74209521501055	36.0	36.0	36.0	32.0	36.0
31	34.72656565784366	36.0	36.0	36.0	32.0	36.0
32	34.686811163488194	36.0	36.0	36.0	32.0	36.0
33	34.64898113292984	36.0	36.0	36.0	32.0	36.0
34	34.63677110933685	36.0	36.0	36.0	32.0	36.0
35	34.60253962630775	36.0	36.0	36.0	32.0	36.0
36	34.562175163496185	36.0	36.0	36.0	32.0	36.0
37	34.554776965300384	36.0	36.0	36.0	32.0	36.0
38	34.514980518965956	36.0	36.0	36.0	32.0	36.0
39	34.50597882290738	36.0	36.0	36.0	32.0	36.0
40	34.48059521201398	36.0	36.0	36.0	32.0	36.0
41	34.455019820645305	36.0	36.0	36.0	32.0	36.0
42	34.387654265084564	36.0	36.0	36.0	32.0	36.0
43	34.38195612089362	36.0	36.0	36.0	32.0	36.0
44	34.309008288512416	36.0	36.0	36.0	32.0	36.0
45	34.27539143527043	36.0	36.0	36.0	32.0	36.0
46	34.242223401612954	36.0	36.0	36.0	32.0	36.0
47	34.20312748673685	36.0	36.0	36.0	32.0	36.0
48	34.14034601725891	36.0	36.0	36.0	32.0	36.0
49	34.110293750420354	36.0	36.0	36.0	32.0	36.0
50	33.51278037073563	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	6.0
20	13.0
21	40.0
22	176.0
23	492.0
24	1255.0
25	2922.0
26	5834.0
27	10904.0
28	18024.0
29	27126.0
30	38762.0
31	54627.0
32	78532.0
33	125684.0
34	260115.0
35	877202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21304274280661	18.49371264782703	12.20709303363244	26.086151575733922
2	15.333699138050438	21.001430367473922	38.00555632504768	25.65931416942796
3	17.680734203986397	25.53911945597946	29.253689798549388	27.526456541484762
4	11.968366876049217	16.642216875749558	36.91894011987611	34.47047612832511
5	13.587846445102505	37.655563598201255	33.70681693022054	15.049773026475693
6	32.53067326356865	36.79439840449086	17.510845932816814	13.16408239912367
7	29.24594264161799	31.210694964960084	21.45432292124476	18.089039472177163
8	27.533816291618194	33.36420910198127	19.85320802787742	19.248766578523117
9	27.78172514804858	14.047451460108956	18.78160994550853	39.38921344633393
10	15.3350562661192	27.014929050551274	32.385964468340276	25.264050214989247
11	36.15920975789712	21.47541780508578	23.64020651028123	18.725165926735862
12	24.782098843121155	23.867942809311764	29.633292113739447	21.716666233827635
13	29.07227698575423	20.232282020018523	25.820248796537808	24.875192197689433
14	23.474627342738135	19.9210236296501	26.302860396280252	30.301488631331512
15	24.524666845583234	27.69515280385128	23.25817727524272	24.522003075322765
16	24.791188184677345	26.251256067245958	24.654677231504596	24.302878516572097
17	23.209233164437773	26.266067441468664	26.004899724781698	24.519799669311862
18	23.7999188925166	25.46894841950282	27.38696060693436	23.344172081046217
19	25.06917082246522	25.012702126965337	26.485616131401592	23.43251091916786
20	25.443595484069455	24.611075669938057	26.070282731773066	23.87504611421943
21	26.425751323318575	24.37636385550673	25.33187011933673	23.866014701837962
22	25.37222491914234	24.706318717358112	25.93305112228502	23.988405241214533
23	23.942782218297783	24.742209001658786	26.326012967961	24.988995812082432
24	24.31486484416775	25.275901954503894	26.29753660990697	24.111696591421385
25	24.419406071139612	24.873825910054972	26.317061720256113	24.389706298549303
26	23.981659990610236	25.587842584915776	26.636233172508632	23.79426425196536
27	24.378143246814133	25.4135500575374	25.92306237480288	24.285244320845585
28	23.803688989104042	25.232509665982533	26.56290580145413	24.400895543459296
29	24.069878789573853	25.272194053883435	26.12676684739686	24.531160309145854
30	24.051415169099755	25.417238812033098	26.41094044049977	24.12040557836738
31	24.51764912719998	25.22795623879531	25.59989184898282	24.654502785021883
32	24.153160046375007	25.580969466680788	25.62864968425174	24.637220802692468
33	23.990908655024345	25.151300437918874	26.103587525172344	24.754203381884437
34	24.475186391738298	25.16341740828248	26.308271557912626	24.0531246420666
35	24.975510679740957	25.109694174838097	26.059433632443774	23.855361512977176
36	24.130456209143365	25.711511515777918	25.810073575245475	24.347958699833246
37	24.819749837346993	25.544080129829393	25.53329209016937	24.102877942654246
38	24.228136385824918	25.66321233516672	25.877717863628824	24.230933415379532
39	24.731314549269708	25.44878577949913	25.403369464387147	24.416530206844012
40	24.922736586532025	25.61029939114899	25.83637936419253	23.63058465812646
41	24.102896383083856	25.784162390305166	26.12964534057507	23.98329588603591
42	24.915695320490965	26.016717098533924	25.46047935134674	23.607108229628366
43	24.162882066990743	25.512619031764	25.83685156822268	24.487647333022576
44	24.091629486581873	25.872810814410336	25.465605646933476	24.569954052074316
45	24.318172737564094	26.005327295731504	25.308050875674237	24.36844909103017
46	24.20531889461498	25.751696573823185	25.212242367498767	24.830742164063064
47	24.49107339064654	25.664218314032855	25.538759664111765	24.30594863120884
48	24.929329574898667	26.07690407268083	24.814925061047926	24.17884129137258
49	24.39282632089242	25.98307798033178	25.07265041499302	24.551445283782776
50	24.303508345513933	26.344255363070644	24.89783279672106	24.45440349469436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	34.0
2	32.0
3	114.5
4	197.0
5	228.5
6	260.0
7	283.5
8	307.0
9	437.5
10	568.0
11	906.5
12	1245.0
13	2214.0
14	3183.0
15	4403.5
16	5624.0
17	5962.0
18	6300.0
19	6231.5
20	6163.0
21	6648.5
22	7134.0
23	8118.5
24	9103.0
25	10253.0
26	11403.0
27	14028.0
28	16653.0
29	19604.0
30	22555.0
31	25954.5
32	29354.0
33	34376.5
34	39399.0
35	45220.5
36	51042.0
37	57193.5
38	63345.0
39	67810.0
40	72275.0
41	77861.5
42	83448.0
43	84591.0
44	85734.0
45	92525.5
46	99317.0
47	104184.0
48	109051.0
49	111691.5
50	114332.0
51	111171.5
52	108011.0
53	105864.0
54	103717.0
55	102292.0
56	100867.0
57	96919.0
58	92971.0
59	83688.5
60	74406.0
61	65497.5
62	56589.0
63	48497.5
64	40406.0
65	33994.5
66	27583.0
67	23528.0
68	19473.0
69	17433.0
70	15393.0
71	11995.0
72	8597.0
73	7238.5
74	5880.0
75	4583.0
76	3286.0
77	2921.0
78	2556.0
79	2116.5
80	1677.0
81	1313.5
82	950.0
83	844.0
84	738.0
85	497.5
86	257.0
87	189.5
88	122.0
89	94.0
90	66.0
91	48.0
92	30.0
93	29.0
94	28.0
95	26.0
96	24.0
97	20.5
98	17.0
99	15.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008723348007647246
2	3.329522140323376E-4
3	5.993139852582078E-4
4	0.0
5	0.0
6	1.3318088561293506E-4
7	6.659044280646753E-5
8	0.0
9	9.322661992905454E-4
10	9.322661992905454E-4
11	0.0
12	2.663617712258701E-4
13	5.327235424517402E-4
14	1.3318088561293506E-4
15	0.0057267780813562074
16	3.9954265683880515E-4
17	0.0
18	3.9954265683880515E-4
19	0.0
20	7.324948708711428E-4
21	0.0
22	2.663617712258701E-4
23	0.0010654470849034805
24	3.9954265683880515E-4
25	0.0014649897417422855
26	0.005460416310130337
27	0.00566018763854974
28	0.003928836125581584
29	0.0020643037270004936
30	0.003928836125581584
31	0.007524720037130831
32	0.003063160369097506
33	0.00459474055364626
34	0.0027302081550651686
35	0.002796798597871636
36	0.008323805350808441
37	0.0035958839115492465
38	0.008257214908001975
39	0.0035958839115492465
40	0.002397255941032831
41	0.003396112583129844
42	0.001398399298935818
43	0.001132037527709948
44	0.001132037527709948
45	0.001132037527709948
46	9.322661992905454E-4
47	0.0017979419557746232
48	0.0018645323985810908
49	3.329522140323376E-4
50	7.990853136776103E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1501717.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.958788612995676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99673938832072	41.21729946706646
2	14.533837260295687	15.97524179448542
3	4.7357500839568205	7.808132633644783
4	2.020512547585471	4.44179687970621
5	1.0442063583939187	2.8694158259658695
6	0.6101529689564563	2.0119960825481757
7	0.3963273812781192	1.5247170938444408
8	0.28871310744783996	1.269385811362156
9	0.20503625496395955	1.0141689775108087
>10	1.0338877573215237	10.072031720435746
>50	0.06848407608890168	2.568200836734748
>100	0.0601208732705413	6.599429132759519
>500	0.00525438257185669	1.9897906260143525
>1k	9.775595482524073E-4	0.6383931179213755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1538	0.10241610103634707	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.997713284194026E-4
2	0.0	0.0	0.0	0.0	1.997713284194026E-4
3	0.0	0.0	0.0	0.0	1.997713284194026E-4
4	0.0	0.0	0.0	0.0	1.997713284194026E-4
5	0.0	0.0	0.0	0.0	3.3295221403233766E-4
6	0.0	0.0	0.0	0.0	3.3295221403233766E-4
7	0.0	0.0	0.0	0.0	3.3295221403233766E-4
8	0.0	0.0	0.0	0.0	3.3295221403233766E-4
9	0.0	0.0	0.0	6.659044280646753E-5	3.3295221403233766E-4
10	0.0	0.0	0.0	6.659044280646753E-5	4.661330996452727E-4
11	0.0	0.0	0.0	1.997713284194026E-4	5.327235424517402E-4
12	0.0	0.0	0.0	1.997713284194026E-4	0.0010654470849034805
13	0.0	0.0	0.0	1.997713284194026E-4	0.0012652184133228831
14	0.0	0.0	0.0	1.997713284194026E-4	0.0012652184133228831
15	0.0	0.0	0.0	1.997713284194026E-4	0.0013983992989358182
16	0.0	0.0	0.0	1.997713284194026E-4	0.0014649897417422857
17	0.0	0.0	0.0	2.663617712258701E-4	0.0014649897417422857
18	0.0	0.0	0.0	2.663617712258701E-4	0.0015315801845487533
19	0.0	0.0	0.0	2.663617712258701E-4	0.0015981706273552208
20	0.0	0.0	0.0	3.3295221403233766E-4	0.0017313515129681557
21	0.0	0.0	0.0	3.3295221403233766E-4	0.0018645323985810908
22	0.0	0.0	0.0	3.995426568388052E-4	0.0019311228413875583
23	0.0	0.0	0.0	5.327235424517402E-4	0.0019311228413875583
24	0.0	0.0	0.0	5.993139852582078E-4	0.001997713284194026
25	0.0	0.0	0.0	5.993139852582078E-4	0.001997713284194026
26	0.0	0.0	0.0	7.324948708711429E-4	0.002130894169806961
27	0.0	0.0	0.0	8.656757564840778E-4	0.002264075055419896
28	0.0	0.0	0.0	9.322661992905454E-4	0.0023306654982263634
29	0.0	0.0	0.0	0.0020643037270004936	0.0023306654982263634
30	0.0	0.0	0.0	0.003995426568388052	0.0023306654982263634
31	0.0	0.0	0.0	0.008856528893260182	0.0024638463838392985
32	0.0	0.0	0.0	0.014516716531809921	0.0025304368266457662
33	0.0	0.0	0.0	0.02077621815561787	0.0025304368266457662
34	0.0	0.0	0.0	0.02696912933661935	0.0027302081550651686
35	0.0	0.0	0.0	0.034493849373750184	0.0027302081550651686
36	0.0	0.0	0.0	0.04867761369152777	0.0027302081550651686
37	0.0	0.0	0.0	0.0693206509615327	0.0027302081550651686
38	0.0	0.0	0.0	0.10228292015073413	0.0027967985978716364
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	465	0.0	20.342855	4
ACTAGAC	160	5.2750693E-11	19.248194	3
GTATCAA	3740	0.0	19.11649	1
CTAGCCG	60	0.007412657	18.333445	9
CGCACGA	60	0.007414081	18.332834	10
CCGTCGT	315	0.0	18.158842	9
CGTCGTA	320	0.0	17.874514	10
GTATAGA	185	2.1827873E-11	17.83676	1
ATACGAT	75	0.0012915921	17.598934	6
ATACCGT	350	0.0	17.598934	6
ACCGTCG	340	0.0	17.46953	8
AACGCAG	4265	0.0	16.814926	6
TACCGTC	370	0.0	16.647642	7
ATACTAG	135	1.0865606E-6	16.29531	1
TACGCTA	345	0.0	15.942125	9
GTCGTAG	360	0.0	15.887927	11
TCAATAC	180	5.2750693E-9	15.887399	3
CTTACGC	85	0.0029822544	15.527954	3
ACGTATG	100	4.9419556E-4	15.401118	32
CTATACA	200	1.2787496E-9	15.399068	4
>>END_MODULE