FastQCFastQC Report
Thu 2 Feb 2017
SRR4064114_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064114_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4234812
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT112880.2665525647891807No Hit
TATCAACGCAGAGTACTTTTTTTTT75840.17908705274283723No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52180.12321680395729491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA2200.012.0918349
GCGTTAT2450.012.019341
CGTCGTA4000.011.63825210
ACGGTAT4850.011.5575859
ACCGTCG4050.011.2598538
CGTTATT2550.011.1725732
CGAGCCG5350.011.01070815
GTATCAA66900.010.9758551
GTCCTAC11450.010.8680291
CCGTTAA1158.814204E-710.74045916
CCGTCGT4300.010.6054629
CGAACGA2001.8189894E-1210.45129316
CGACCAT4200.010.40543510
CGCGTAA1752.3646862E-1010.31495310
CGAACGT2053.6379788E-1210.1934934
TCGAACG2053.6379788E-1210.1924093
TAAGGCG1409.717587E-810.17754655
CGGAATT1606.171831E-910.09499915
GTATAAA3800.09.9991111
GTCGTAG4850.09.99010111
Produced by FastQC (version 0.11.5)