##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064114_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4234812
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.232353171758273	32.0	32.0	32.0	32.0	32.0
2	30.735141253023748	32.0	32.0	32.0	32.0	32.0
3	30.759865373008296	32.0	32.0	32.0	32.0	32.0
4	30.792584416970577	32.0	32.0	32.0	32.0	32.0
5	30.665708182559225	32.0	32.0	32.0	32.0	32.0
6	34.35083588126226	36.0	36.0	36.0	32.0	36.0
7	34.28381991927859	36.0	36.0	36.0	32.0	36.0
8	34.22687878470166	36.0	36.0	36.0	32.0	36.0
9	34.41668602998197	36.0	36.0	36.0	32.0	36.0
10	34.02800879944611	36.0	36.0	36.0	32.0	36.0
11	34.40829415804055	36.0	36.0	36.0	32.0	36.0
12	34.1759069824115	36.0	36.0	36.0	32.0	36.0
13	34.27877955385033	36.0	36.0	36.0	32.0	36.0
14	34.15513557626643	36.0	36.0	36.0	32.0	36.0
15	34.08317299563711	36.0	36.0	36.0	32.0	36.0
16	34.085893541437024	36.0	36.0	36.0	32.0	36.0
17	34.01146780541852	36.0	36.0	36.0	32.0	36.0
18	34.005142377040585	36.0	36.0	36.0	32.0	36.0
19	34.02745198606219	36.0	36.0	36.0	32.0	36.0
20	34.00825018914653	36.0	36.0	36.0	32.0	36.0
21	33.998746343403205	36.0	36.0	36.0	32.0	36.0
22	33.97169602806453	36.0	36.0	36.0	32.0	36.0
23	33.90617151363508	36.0	36.0	36.0	32.0	36.0
24	33.90226224918603	36.0	36.0	36.0	32.0	36.0
25	33.48360730063106	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	10.0
4	28.0
5	49.0
6	194.0
7	63.0
8	259.0
9	253.0
10	354.0
11	128.0
12	262.0
13	222.0
14	457.0
15	736.0
16	1195.0
17	1606.0
18	2138.0
19	2974.0
20	4671.0
21	6794.0
22	10885.0
23	17078.0
24	25069.0
25	36812.0
26	51793.0
27	68441.0
28	92191.0
29	121646.0
30	159904.0
31	216290.0
32	303523.0
33	428347.0
34	894487.0
35	1785951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.203079297000315	17.36673238567562	11.61424028400439	25.81594803331968
2	16.240847384700544	20.017300408871524	38.15665867374867	25.585193532679256
3	18.44002162110594	24.45178004562724	29.08912472067425	28.01907361259257
4	12.47984784607214	15.954413330565695	36.57267326601741	34.993065557344764
5	14.26044184962924	36.99815819235991	34.00677352567419	14.734626432336654
6	33.17823954020252	36.074622621635385	17.512987068195923	13.234150769966172
7	29.70059645462252	30.963253076218315	21.2371904443496	18.098960024809564
8	27.851073474674305	33.34067983225415	19.683987159926193	19.12425953314535
9	27.64666691867168	14.346283241036497	18.568272883630385	39.438776956661435
10	15.364284851882909	27.201011444912186	32.38752554425296	25.047178158951944
11	36.294373427638774	21.274106388488285	23.486752945312546	18.94476723856039
12	24.76162684230735	23.688359866497326	29.490173376258973	22.05983991493635
13	29.361021503597794	20.119228664406446	25.51599277510872	25.00375705688704
14	23.4302833072854	19.820852932294443	25.950851878404762	30.7980118820154
15	24.791784068341656	27.588867899339952	22.77125841600183	24.84808961631656
16	25.107864318396047	26.092047211477865	24.364729437134816	24.435359032991276
17	23.60611493792248	26.197742601357994	25.718338386740662	24.47780407397886
18	24.056451835885188	25.610912061701036	26.665384221816325	23.667251880597455
19	25.244327462605305	25.169533341399596	25.878553252469032	23.707585943526066
20	25.442725337655247	24.886487196738905	25.46874471961339	24.20204274599246
21	26.195158044038276	24.517651206849735	24.99909609449015	24.28809465462184
22	25.467190828816626	24.76614167738978	25.333408956366693	24.4332585374269
23	24.347847254291477	24.747535860188396	25.734326189576993	25.17029069594313
24	24.44313837534299	25.18492733237907	25.811911397469515	24.560022894808423
25	24.641904843380118	24.90590187326583	25.82401883782226	24.628174445531794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	596.0
1	596.0
2	1214.5
3	1833.0
4	1833.0
5	1833.0
6	4783.0
7	7733.0
8	7733.0
9	7733.0
10	8439.5
11	9146.0
12	9146.0
13	9146.0
14	11472.0
15	13798.0
16	13798.0
17	13798.0
18	24306.0
19	34814.0
20	34814.0
21	34814.0
22	53217.5
23	71621.0
24	71621.0
25	71621.0
26	103232.5
27	134844.0
28	134844.0
29	134844.0
30	170807.0
31	206770.0
32	206770.0
33	206770.0
34	245976.0
35	285182.0
36	285182.0
37	285182.0
38	324779.5
39	364377.0
40	364377.0
41	364377.0
42	410424.0
43	456471.0
44	456471.0
45	456471.0
46	502325.0
47	548179.0
48	548179.0
49	548179.0
50	556966.5
51	565754.0
52	565754.0
53	565754.0
54	526744.5
55	487735.0
56	487735.0
57	487735.0
58	442079.5
59	396424.0
60	396424.0
61	396424.0
62	342252.5
63	288081.0
64	288081.0
65	288081.0
66	234356.0
67	180631.0
68	180631.0
69	180631.0
70	135639.0
71	90647.0
72	90647.0
73	90647.0
74	68155.0
75	45663.0
76	45663.0
77	45663.0
78	35195.5
79	24728.0
80	24728.0
81	24728.0
82	17116.0
83	9504.0
84	9504.0
85	9504.0
86	7192.5
87	4881.0
88	4881.0
89	4881.0
90	3585.5
91	2290.0
92	2290.0
93	2290.0
94	1601.0
95	912.0
96	912.0
97	912.0
98	1555.0
99	2198.0
100	2198.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038679403005375444
2	0.005431173804173597
3	0.0014404417480634324
4	0.0036129112697328714
5	0.00909131267220363
6	0.014168279489148514
7	0.022692860981786207
8	0.03549154012031703
9	0.04940006781883116
10	0.05787742171317168
11	0.05487846922130192
12	0.06345027831223676
13	0.06555190643646046
14	0.06777160355642706
15	0.060144346431435444
16	0.06788967255216997
17	0.06337943691479102
18	0.07818528898095121
19	0.07546970207886443
20	0.07912984094689444
21	0.07497381229674423
22	0.07785469579287109
23	0.08307334540470747
24	0.07757133020308811
25	0.07851588216903135
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4234812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.15892065603114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3280752893947	31.7593997188516
2	15.610081746031291	14.09868886006374
3	5.671446452347899	7.683492010395243
4	2.6785341921454506	4.838388522302515
5	1.4710606583139547	3.321575577450441
6	0.9199154985051614	2.492543460434875
7	0.619486197019949	1.9582729613111245
8	0.43471869185482265	1.5705141530532467
9	0.31213153235164837	1.2685970793342156
>10	1.7565077678907213	14.486615659056024
>50	0.11430875138498703	3.551333590868111
>100	0.0702571576821519	6.421996016484462
>500	0.008738386095504399	2.720640150916111
>1k	0.0045797563490928535	3.2552735034959897
>5k	1.0528175515155874E-4	0.30432981145895827
>10k+	5.264087757577937E-5	0.26833892452341207
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11288	0.2665525647891807	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7584	0.17908705274283723	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5218	0.12321680395729491	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8891039318864688E-4
2	0.0	0.0	0.0	0.0	1.8891039318864688E-4
3	0.0	0.0	0.0	0.0	1.8891039318864688E-4
4	0.0	0.0	0.0	0.0	1.8891039318864688E-4
5	0.0	0.0	0.0	0.0	2.1252419233722773E-4
6	0.0	0.0	0.0	0.0	2.833655897829703E-4
7	0.0	0.0	0.0	0.0	2.833655897829703E-4
8	0.0	0.0	0.0	0.0	2.833655897829703E-4
9	0.0	0.0	0.0	2.361379914858086E-5	3.0697938893155117E-4
10	0.0	0.0	0.0	2.5975179063438946E-4	3.30593188080132E-4
11	0.0	0.0	0.0	2.833655897829703E-4	3.5420698722871287E-4
12	0.0	0.0	0.0	2.833655897829703E-4	4.95889782120198E-4
13	0.0	0.0	0.0	2.833655897829703E-4	5.903449787145215E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTA	220	0.0	12.091834	9
GCGTTAT	245	0.0	12.01934	1
CGTCGTA	400	0.0	11.638252	10
ACGGTAT	485	0.0	11.557585	9
ACCGTCG	405	0.0	11.259853	8
CGTTATT	255	0.0	11.172573	2
CGAGCCG	535	0.0	11.010708	15
GTATCAA	6690	0.0	10.975855	1
GTCCTAC	1145	0.0	10.868029	1
CCGTTAA	115	8.814204E-7	10.740459	16
CCGTCGT	430	0.0	10.605462	9
CGAACGA	200	1.8189894E-12	10.451293	16
CGACCAT	420	0.0	10.405435	10
CGCGTAA	175	2.3646862E-10	10.314953	10
CGAACGT	205	3.6379788E-12	10.193493	4
TCGAACG	205	3.6379788E-12	10.192409	3
TAAGGCG	140	9.717587E-8	10.1775465	5
CGGAATT	160	6.171831E-9	10.094999	15
GTATAAA	380	0.0	9.999111	1
GTCGTAG	485	0.0	9.990101	11
>>END_MODULE