FastQCFastQC Report
Thu 2 Feb 2017
SRR4064114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4234812
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA82500.021.6795231
TAGGACG11100.018.8281924
AACGCAG101900.017.3578116
GTCCTAC12350.016.744571
GGTATCA42700.016.5897981
TCAACGC112950.015.6010674
GTAGGAC16250.015.56833653
ATCAACG114450.015.3962333
TATCAAC116200.015.3722482
CGTCGTA8050.015.30437310
CAACGCA115250.015.2899035
TGGCGAG16050.015.0766618
AGGACGT13150.014.8894065
TGTAGGA19600.014.8151252
TCCTACA15950.014.7574252
ACGCAGA119750.014.7153337
ACCGTCG7550.014.5692158
TTAGGAC11350.014.5366113
AATCACG7300.014.46697234
CGCAGAG122750.014.3556928
Produced by FastQC (version 0.11.5)