##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064114_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4234812
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298896621620983	32.0	32.0	32.0	32.0	32.0
2	31.42654006836667	32.0	32.0	32.0	32.0	32.0
3	31.50042929886852	32.0	32.0	32.0	32.0	32.0
4	31.584977089892067	32.0	32.0	32.0	32.0	32.0
5	31.544597729485986	32.0	32.0	32.0	32.0	32.0
6	35.1394109112754	36.0	36.0	36.0	36.0	36.0
7	35.15303276745225	36.0	36.0	36.0	36.0	36.0
8	35.10938974386584	36.0	36.0	36.0	36.0	36.0
9	35.20000061395878	36.0	36.0	36.0	36.0	36.0
10	35.071875681848454	36.0	36.0	36.0	36.0	36.0
11	35.20998618120474	36.0	36.0	36.0	36.0	36.0
12	35.119186164580626	36.0	36.0	36.0	36.0	36.0
13	35.15357801007459	36.0	36.0	36.0	36.0	36.0
14	35.10213204269753	36.0	36.0	36.0	36.0	36.0
15	35.08180127004457	36.0	36.0	36.0	36.0	36.0
16	35.09245558008242	36.0	36.0	36.0	36.0	36.0
17	35.063217918528615	36.0	36.0	36.0	36.0	36.0
18	35.05431764149152	36.0	36.0	36.0	36.0	36.0
19	35.05634181635454	36.0	36.0	36.0	36.0	36.0
20	35.04626486370587	36.0	36.0	36.0	36.0	36.0
21	35.04434081135125	36.0	36.0	36.0	36.0	36.0
22	35.02725079649345	36.0	36.0	36.0	36.0	36.0
23	34.977701253326	36.0	36.0	36.0	36.0	36.0
24	34.95630573446944	36.0	36.0	36.0	36.0	36.0
25	34.931243464881085	36.0	36.0	36.0	36.0	36.0
26	34.874462668000376	36.0	36.0	36.0	32.0	36.0
27	34.87061125735924	36.0	36.0	36.0	32.0	36.0
28	34.84236938971553	36.0	36.0	36.0	32.0	36.0
29	34.82032921414221	36.0	36.0	36.0	32.0	36.0
30	34.80229842552633	36.0	36.0	36.0	32.0	36.0
31	34.792582055590664	36.0	36.0	36.0	32.0	36.0
32	34.75024463895918	36.0	36.0	36.0	32.0	36.0
33	34.73170851504152	36.0	36.0	36.0	32.0	36.0
34	34.71839340211561	36.0	36.0	36.0	32.0	36.0
35	34.682932323796194	36.0	36.0	36.0	32.0	36.0
36	34.658782255268946	36.0	36.0	36.0	32.0	36.0
37	34.64244315922407	36.0	36.0	36.0	32.0	36.0
38	34.609445236293844	36.0	36.0	36.0	32.0	36.0
39	34.605054250342164	36.0	36.0	36.0	32.0	36.0
40	34.5831345051445	36.0	36.0	36.0	32.0	36.0
41	34.56684735945775	36.0	36.0	36.0	32.0	36.0
42	34.50601230940122	36.0	36.0	36.0	32.0	36.0
43	34.49416928071423	36.0	36.0	36.0	32.0	36.0
44	34.44246356154653	36.0	36.0	36.0	32.0	36.0
45	34.4031645324515	36.0	36.0	36.0	32.0	36.0
46	34.37895684625433	36.0	36.0	36.0	32.0	36.0
47	34.33544818518508	36.0	36.0	36.0	32.0	36.0
48	34.295331882501515	36.0	36.0	36.0	32.0	36.0
49	34.271246515783936	36.0	36.0	36.0	32.0	36.0
50	33.69379797733642	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	10.0
19	10.0
20	52.0
21	178.0
22	505.0
23	1468.0
24	3575.0
25	8149.0
26	16062.0
27	28735.0
28	47566.0
29	71252.0
30	101920.0
31	143163.0
32	203933.0
33	326805.0
34	685994.0
35	2595429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2920425624067	18.21508435233836	12.130314249271628	26.362558835983307
2	15.506374099897185	20.65951732244827	37.697677856349095	26.136430721305455
3	17.713799943326723	24.989255690941718	29.343321998677624	27.95362236705393
4	12.126890166552847	16.63448105842715	36.62138956817918	34.61723920684082
5	13.861465396811004	37.40813051441245	33.81160722128869	14.91879686748786
6	33.17876501987695	36.4361995416554	17.289792564238496	13.095242874229157
7	29.498048200048693	31.14423814627767	21.28610759068473	18.07160606298891
8	27.78567921921427	33.51460076966835	19.56488731138178	19.134832699735597
9	27.59343526772537	14.180270381958792	18.461656532262825	39.76463781805301
10	15.245826693857426	27.379561402597353	32.44703610961818	24.92757579392704
11	36.40740991746739	21.249330843808615	23.457079902739462	18.886179335984536
12	24.642752314332153	23.73201423347038	29.569234205950828	22.055999246246643
13	29.444128280268917	20.06907072133447	25.55293178646403	24.93386921193259
14	23.45040534372217	19.759322811154526	25.953569010680784	30.836702834442516
15	24.82493124533902	27.627684360020798	22.77024962322493	24.777134771415252
16	25.1019122884861	26.138238000703222	24.357597781602728	24.402251929207942
17	23.699989515473177	26.240834303860478	25.66992348184524	24.389252698821103
18	24.16284692614691	25.507444391103334	26.73420863658464	23.595500046165117
19	25.22797357426341	25.138075819676487	25.951854758099003	23.6820958479611
20	25.482551176163888	24.793968240174706	25.54959119520618	24.173889388455233
21	26.17939119847587	24.495420339793124	25.14456367838761	24.180624783343394
22	25.385046972613633	24.74043687458794	25.447434688892624	24.4270814639058
23	24.34786428001657	24.644597662498484	25.83986693045066	25.167671127034286
24	24.520312808825746	25.094721321548104	25.86895079355746	24.51601507606869
25	24.65810681155748	24.77613089290956	25.9676357093925	24.59812658614046
26	24.376024157301117	25.388261737471424	26.094636562454028	24.141077542773438
27	24.728117381123084	25.29561596656833	25.498872606946478	24.477394045362104
28	24.35432859076239	25.16989115678066	25.870118780349515	24.605661472107435
29	24.434683305051166	25.128356018103737	25.733380310226966	24.70358036661813
30	24.34841522425653	25.290833665317713	25.932069265913466	24.428681844512294
31	24.828621385746175	24.964789098270295	25.35584028656148	24.85074922942205
32	24.46102032083743	25.39247468750797	25.27860507600596	24.867899915648643
33	24.29176337900982	25.132567443207055	25.694244866797316	24.881424310985807
34	24.75632837784067	25.023643969636538	25.867789769983435	24.352237882539356
35	25.059904158069994	25.072585160487886	25.780690446340586	24.086820235101534
36	24.436672803581263	25.38965786622408	25.527455057097864	24.646214273096792
37	25.00895590765202	25.102848297432956	25.346151439262183	24.54204435565285
38	24.505570231391193	25.282974933840663	25.608587985190134	24.60286684957801
39	24.864279343326682	25.06120388219289	25.312985802494065	24.761530971986367
40	25.17230817985368	25.18565030088254	25.61271624567355	24.029325273590228
41	24.44239994370276	25.329860725733745	25.89023549909249	24.337503831471004
42	25.08295084204757	25.545199146960012	25.474687093909253	23.89716291708316
43	24.45352749193518	24.96385265627188	25.840881789743953	24.741738062048984
44	24.339318643312946	25.362116409512993	25.440727767850667	24.857837179323393
45	24.360856518974234	25.481814946694847	25.477375507377143	24.679953026953772
46	24.384286822122792	25.19238380226147	25.463827500186554	24.959501875429186
47	24.558407397498165	25.26664634265853	25.71005133976696	24.46489492007635
48	24.781456218629973	25.585331785814226	25.081758362683853	24.55145363287195
49	24.42764080545065	25.512368125319824	25.271223154262174	24.78876791496735
50	24.351149310338233	25.82315821191157	25.172754894790895	24.652937582959307
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	203.0
1	165.5
2	128.0
3	435.0
4	742.0
5	849.5
6	957.0
7	890.5
8	824.0
9	1162.5
10	1501.0
11	2199.5
12	2898.0
13	5214.0
14	7530.0
15	9948.5
16	12367.0
17	12853.5
18	13340.0
19	13521.5
20	13703.0
21	15478.0
22	17253.0
23	20176.5
24	23100.0
25	27360.5
26	31621.0
27	39027.0
28	46433.0
29	54721.0
30	63009.0
31	72063.5
32	81118.0
33	92983.5
34	104849.0
35	120197.5
36	135546.0
37	152664.5
38	169783.0
39	182985.0
40	196187.0
41	210955.5
42	225724.0
43	233171.5
44	240619.0
45	258041.0
46	275463.0
47	289958.5
48	304454.0
49	311839.5
50	319225.0
51	315789.5
52	312354.0
53	309032.0
54	305710.0
55	299146.5
56	292583.0
57	280680.0
58	268777.0
59	245646.0
60	222515.0
61	195364.0
62	168213.0
63	145162.0
64	122111.0
65	103276.0
66	84441.0
67	71163.5
68	57886.0
69	50564.0
70	43242.0
71	33812.5
72	24383.0
73	20675.5
74	16968.0
75	13292.0
76	9616.0
77	8285.0
78	6954.0
79	5709.5
80	4465.0
81	3600.5
82	2736.0
83	2184.5
84	1633.0
85	1197.0
86	761.0
87	537.5
88	314.0
89	249.0
90	184.0
91	136.0
92	88.0
93	89.5
94	91.0
95	95.0
96	99.0
97	83.5
98	68.0
99	55.5
100	43.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008760719484123498
2	4.250483846744554E-4
3	2.8336558978297034E-4
4	0.0
5	0.0
6	1.6529659404006602E-4
7	7.084139744574259E-5
8	2.361379914858086E-5
9	0.0014640555472120132
10	9.445519659432342E-4
11	2.361379914858086E-5
12	1.1806899574290428E-4
13	5.195035812687788E-4
14	2.597517906343894E-4
15	0.004486621838230363
16	0.001015393363388977
17	0.0
18	3.0697938893155117E-4
19	2.361379914858086E-5
20	6.611863761602641E-4
21	0.0
22	9.445519659432344E-5
23	8.028691710517492E-4
24	4.958897821201979E-4
25	0.001959945329332211
26	0.00550201520161934
27	0.006234042975225347
28	0.004463008039081782
29	0.002503062709749571
30	0.00413241485100165
31	0.007745326120734522
32	0.0034003870773956432
33	0.00413241485100165
34	0.0027628145003839606
35	0.0030934076884640924
36	0.007745326120734522
37	0.0041796424492988125
38	0.00798146411222033
39	0.004604690833973267
40	0.0024322213123038282
41	0.0034476146756928054
42	0.0017710349361435642
43	0.0014404417480634324
44	0.001298758953171947
45	0.001015393363388977
46	0.0011334623591318814
47	0.001959945329332211
48	0.0018182625344407262
49	3.5420698722871287E-4
50	0.001298758953171947
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4234812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.71662103076844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.94705445320811	35.05017386077001
2	14.965909745223872	14.58177106877511
3	5.397020190668414	7.8877376197259625
4	2.5048012817905776	4.8810181920949836
5	1.400904781196265	3.4123673662865004
6	0.8517608942091317	2.489694761520883
7	0.5308402850644001	1.8102521496743227
8	0.38687372144390014	1.5077744379476445
9	0.3066734844949863	1.344608633189474
>10	1.5466521885115487	13.603537095265605
>50	0.09515957622681417	3.210210565292209
>100	0.05667809170034005	5.633224598256953
>500	0.006349847785581093	2.0865431440251805
>1k	0.003321458476014558	2.501086507175298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4168279489148514E-4
2	0.0	0.0	0.0	0.0	1.4168279489148514E-4
3	0.0	0.0	0.0	0.0	1.4168279489148514E-4
4	0.0	0.0	0.0	0.0	1.4168279489148514E-4
5	0.0	0.0	0.0	0.0	1.65296594040066E-4
6	0.0	0.0	0.0	0.0	1.65296594040066E-4
7	0.0	0.0	0.0	0.0	1.65296594040066E-4
8	0.0	0.0	0.0	0.0	1.65296594040066E-4
9	0.0	0.0	0.0	2.361379914858086E-5	2.3613799148580858E-4
10	0.0	0.0	0.0	7.084139744574257E-5	2.5975179063438946E-4
11	0.0	0.0	0.0	9.445519659432344E-5	2.5975179063438946E-4
12	0.0	0.0	0.0	9.445519659432344E-5	5.903449787145215E-4
13	0.0	0.0	0.0	1.1806899574290429E-4	7.084139744574257E-4
14	0.0	0.0	0.0	1.1806899574290429E-4	7.556415727545875E-4
15	0.0	0.0	0.0	1.65296594040066E-4	9.91779564240396E-4
16	0.0	0.0	0.0	2.1252419233722773E-4	9.91779564240396E-4
17	0.0	0.0	0.0	2.3613799148580858E-4	9.91779564240396E-4
18	0.0	0.0	0.0	3.30593188080132E-4	0.0010862347608347194
19	2.361379914858086E-5	0.0	0.0	3.5420698722871287E-4	0.0010862347608347194
20	2.361379914858086E-5	0.0	0.0	4.2504838467445546E-4	0.0011098485599833003
21	2.361379914858086E-5	0.0	0.0	5.195035812687789E-4	0.0011334623591318811
22	2.361379914858086E-5	0.0	0.0	7.792553719031683E-4	0.0011334623591318811
23	2.361379914858086E-5	0.0	0.0	0.0013696003506176897	0.0011334623591318811
24	2.361379914858086E-5	0.0	0.0	0.0022905385174123434	0.001157076158280462
25	2.361379914858086E-5	0.0	0.0	0.0027628145003839606	0.001157076158280462
26	2.361379914858086E-5	0.0	0.0	0.003164249085909835	0.001180689957429043
27	2.361379914858086E-5	0.0	0.0	0.0038962768595158415	0.0012043037565776238
28	2.361379914858086E-5	0.0	0.0	0.006210429176076766	0.0012043037565776238
29	2.361379914858086E-5	0.0	0.0	0.011688830578547525	0.0012043037565776238
30	2.361379914858086E-5	0.0	0.0	0.021039895041385544	0.0012043037565776238
31	2.361379914858086E-5	0.0	0.0	0.047770715677579074	0.0012043037565776238
32	2.361379914858086E-5	0.0	0.0	0.07608366085672752	0.0012279175557262047
33	2.361379914858086E-5	0.0	0.0	0.10186992952697782	0.0012279175557262047
34	2.361379914858086E-5	0.0	0.0	0.1320247510397156	0.0013459865514691088
35	2.361379914858086E-5	0.0	0.0	0.16916925710043326	0.0013459865514691088
36	2.361379914858086E-5	0.0	0.0	0.21996253906903068	0.0013459865514691088
37	2.361379914858086E-5	0.0	0.0	0.29821866944742764	0.0013459865514691088
38	2.361379914858086E-5	0.0	0.0	0.4021666132994806	0.0014168279489148515
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8250	0.0	21.679523	1
TAGGACG	1110	0.0	18.828192	4
AACGCAG	10190	0.0	17.357811	6
GTCCTAC	1235	0.0	16.74457	1
GGTATCA	4270	0.0	16.589798	1
TCAACGC	11295	0.0	15.601067	4
GTAGGAC	1625	0.0	15.5683365	3
ATCAACG	11445	0.0	15.396233	3
TATCAAC	11620	0.0	15.372248	2
CGTCGTA	805	0.0	15.304373	10
CAACGCA	11525	0.0	15.289903	5
TGGCGAG	1605	0.0	15.07666	18
AGGACGT	1315	0.0	14.889406	5
TGTAGGA	1960	0.0	14.815125	2
TCCTACA	1595	0.0	14.757425	2
ACGCAGA	11975	0.0	14.715333	7
ACCGTCG	755	0.0	14.569215	8
TTAGGAC	1135	0.0	14.536611	3
AATCACG	730	0.0	14.466972	34
CGCAGAG	12275	0.0	14.355692	8
>>END_MODULE