FastQCFastQC Report
Thu 2 Feb 2017
SRR4064113_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064113_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT261.4372581536760642No Hit
TATCAACGCAGAGTACTTTTTTTTT160.8844665561083471No Hit
GGTATCAACGCAGAGTACTTTTTTT150.8291873963515755No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80.44223327805417356No Hit
GTGCACACGAGGCCAGAGACCCTGG60.33167495854063017No Hit
TTCGTATTGCGCCGCTAGAGGTGAA40.22111663902708678No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG40.22111663902708678No Hit
GTACTTTTTTTTTTTTTTTTTTTTT40.22111663902708678No Hit
GCAGAGTACTTTTTTTTTTTTTTTT40.22111663902708678No Hit
ATCAACGCAGAGTACTTTTTTTTTT40.22111663902708678No Hit
GTTTTAATTAGACAGTCAGATTCCC30.16583747927031509No Hit
TCATCCCACACGGTCGCCATCTTCT30.16583747927031509No Hit
TCGTAGTTCCGACCATAAACGATGC30.16583747927031509No Hit
CTTCGGGCCCCGCGGGACACTCAGC30.16583747927031509No Hit
TATCAACGCAGAGTACATGGGCAGA30.16583747927031509No Hit
GCCGAGTCCCGGCCTGTCTCTGAGG30.16583747927031509No Hit
TATCTACATTGTTCTATCAACTAGA30.16583747927031509No Hit
TAACAATACAGGACTCTTTCGAGGC20.11055831951354339No Hit
GTATCAACGCAGAGTACATGGGGTG20.11055831951354339No Hit
GTCATATACACCATCTCGAAGCCCC20.11055831951354339No Hit
CTACCATGGTGACCACGGGTGACGG20.11055831951354339No Hit
GCGACGACCCATTCGAACGTCTGCC20.11055831951354339No Hit
GTTGTTAACTGTGCGCCGGACGGCC20.11055831951354339No Hit
ATACAGAGAACCCTGTCTCGAAAAA20.11055831951354339No Hit
GTATCAACGCAGAGTACATGGGCAG20.11055831951354339No Hit
GTTCGAAGACGATCAGATACCGTCG20.11055831951354339No Hit
ACCATACTCCCCCCGGAACCCAAAG20.11055831951354339No Hit
GGAGGGAGCTCACCGGGTTGGTTTT20.11055831951354339No Hit
GTACATGGGCGGACTACCGATGCTT20.11055831951354339No Hit
TGATTAATGAAAACATTCTTGGCAA20.11055831951354339No Hit
TGTCAATCCTGTCCGTGTCCGGGCC20.11055831951354339No Hit
CCTCAGAGCTCATTGTGCCGAGCCC20.11055831951354339No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT20.11055831951354339No Hit
TTGTTATACTGTCTCCGGCCTCTCT20.11055831951354339No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20.11055831951354339No Hit
GTGCAGTTCCATGGGCTTTCAGAGG20.11055831951354339No Hit
ATGGTAGGCACGGCGACTACCATCG20.11055831951354339No Hit
GCATATGCTTGTCTCAAAGATTAAG20.11055831951354339No Hit
CTTTAGGACGTGAAATATGGCGAGG20.11055831951354339No Hit
ACATCTTTGTGTGTAACAACATTTT20.11055831951354339No Hit
GTGAAGAGGAGGCAGCCTCAGCTGG20.11055831951354339No Hit
CTATCAATCTGTCAATCCTGTCCGT20.11055831951354339No Hit
GGCTCCACTCCTGGTGGTGCCCTTC20.11055831951354339No Hit
GAACTACGACGGTATCTGATCGTCT20.11055831951354339No Hit
GGTACGGACAAGGGGAATCTGACTG20.11055831951354339No Hit
GCTAAGAGCATCGAGGGGGCGCCGA20.11055831951354339No Hit
GTCCTAAGGTCTCCCGAGGCCTGCT20.11055831951354339No Hit
GGTATCAACGCAGAGTACATGGGAG20.11055831951354339No Hit
TCGCTCCCCAGCCCCTGGCCTGGCA20.11055831951354339No Hit
GTCCTGTATTGTTATTTTTCGTCAC20.11055831951354339No Hit
GTCCCAATTCGCGTCATAAACTAAT20.11055831951354339No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20.11055831951354339No Hit
ATGCTATGTTTTAATTAGACAGTCA20.11055831951354339No Hit
CTAGATAACCTCGGGCCGATCGCAC20.11055831951354339No Hit
AGGTTATCTAGAGTCACCAAAGCCG20.11055831951354339No Hit
GATATAAAGAAGGTGATGTGTACAT20.11055831951354339No Hit
CCACGGGTGACGGGGAATCAGGGTT20.11055831951354339No Hit
CAGAGCTCATTGTGCCGAGCCCTTG20.11055831951354339No Hit
GGCCGAATGTGCTCGCAATTGCCAT20.11055831951354339No Hit
ACGCAGAGTACATGGGAGAAATCGT20.11055831951354339No Hit
CATTGTGCCGAGCCCTTGAGACCCT20.11055831951354339No Hit
GTATCAACGCAGAGTACATGGGTGA20.11055831951354339No Hit
GTACTGTTTGATCCACTTTGACCCA20.11055831951354339No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)