##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064113_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1809
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.800995024875622	32.0	32.0	32.0	21.0	32.0
2	29.836373687119956	32.0	32.0	32.0	21.0	32.0
3	30.598120508568268	32.0	32.0	32.0	32.0	32.0
4	30.998341625207296	32.0	32.0	32.0	32.0	32.0
5	30.60585959093422	32.0	32.0	32.0	32.0	32.0
6	34.14648977335545	36.0	36.0	36.0	32.0	36.0
7	34.07517965726921	36.0	36.0	36.0	32.0	36.0
8	34.07462686567164	36.0	36.0	36.0	32.0	36.0
9	34.6661138750691	36.0	36.0	36.0	32.0	36.0
10	34.00331674958541	36.0	36.0	36.0	32.0	36.0
11	34.6865671641791	36.0	36.0	36.0	32.0	36.0
12	34.35599778883361	36.0	36.0	36.0	32.0	36.0
13	34.591487009397454	36.0	36.0	36.0	32.0	36.0
14	34.40685461580984	36.0	36.0	36.0	32.0	36.0
15	34.08678828081813	36.0	36.0	36.0	32.0	36.0
16	34.24156992813709	36.0	36.0	36.0	32.0	36.0
17	33.93919292426755	36.0	36.0	36.0	32.0	36.0
18	34.2277501381979	36.0	36.0	36.0	32.0	36.0
19	34.21558872305141	36.0	36.0	36.0	32.0	36.0
20	34.34715312327253	36.0	36.0	36.0	32.0	36.0
21	34.281923714759536	36.0	36.0	36.0	32.0	36.0
22	34.30348258706468	36.0	36.0	36.0	32.0	36.0
23	34.24599226091763	36.0	36.0	36.0	32.0	36.0
24	34.22553897180763	36.0	36.0	36.0	32.0	36.0
25	34.03869541182974	36.0	36.0	36.0	32.0	36.0
26	33.80320619126589	36.0	36.0	36.0	32.0	36.0
27	33.91929242675511	36.0	36.0	36.0	32.0	36.0
28	34.022111663902706	36.0	36.0	36.0	32.0	36.0
29	33.8595909342178	36.0	36.0	36.0	32.0	36.0
30	33.85461580983969	36.0	36.0	36.0	32.0	36.0
31	33.791044776119406	36.0	36.0	36.0	32.0	36.0
32	33.67053620784964	36.0	36.0	36.0	27.0	36.0
33	33.728579325594254	36.0	36.0	36.0	27.0	36.0
34	33.90049751243781	36.0	36.0	36.0	32.0	36.0
35	33.78054173576562	36.0	36.0	36.0	32.0	36.0
36	33.70259812050857	36.0	36.0	36.0	27.0	36.0
37	33.66113875069099	36.0	36.0	36.0	32.0	36.0
38	33.6301824212272	36.0	36.0	36.0	32.0	36.0
39	33.63128800442233	36.0	36.0	36.0	27.0	36.0
40	33.56274184632394	36.0	36.0	36.0	21.0	36.0
41	33.54228855721393	36.0	36.0	36.0	21.0	36.0
42	33.550580431177444	36.0	36.0	36.0	21.0	36.0
43	33.49364289662797	36.0	36.0	36.0	21.0	36.0
44	33.43559977888336	36.0	36.0	36.0	21.0	36.0
45	33.40464344941957	36.0	36.0	36.0	21.0	36.0
46	33.50525152017689	36.0	36.0	36.0	21.0	36.0
47	33.076285240464344	36.0	36.0	36.0	14.0	36.0
48	33.27031509121061	36.0	36.0	36.0	21.0	36.0
49	33.384190160309565	36.0	36.0	36.0	21.0	36.0
50	32.16252072968491	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	0.0
23	2.0
24	4.0
25	9.0
26	11.0
27	26.0
28	38.0
29	58.0
30	79.0
31	135.0
32	183.0
33	264.0
34	399.0
35	599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.46932006633499	21.06135986733002	13.764510779436154	25.70480928689884
2	18.076285240464347	19.237147595356554	36.42896627971255	26.25760088446656
3	20.453289110005528	25.31785516860144	28.026533996683252	26.202321724709783
4	16.362631288004422	15.58872305140962	35.1575456053068	32.89110005527916
5	16.30735212824765	33.77556661138751	31.288004422332783	18.629076838032063
6	34.54947484798231	35.43394140409066	15.809839690436705	14.206744057490326
7	30.016583747927033	29.132117191818686	20.17689331122167	20.674405749032616
8	30.514096185737976	31.01160862354892	19.181868435599778	19.292426755113322
9	29.629629629629626	13.156440022111665	16.749585406301822	40.46434494195688
10	15.865118850193477	25.04145936981758	31.4538419016031	27.63957987838585
11	37.81094527363184	21.83526810392482	19.900497512437813	20.453289110005528
12	25.649530127142068	20.453289110005528	28.800442233278055	25.096738529574353
13	30.458817025981205	18.407960199004975	23.383084577114428	27.750138197899393
14	23.272526257600884	18.90547263681592	25.76008844665561	32.06191265892758
15	23.991155334438915	25.594250967385296	23.991155334438915	26.423438363736874
16	26.478717523493643	26.920950801547818	21.724709784411278	24.875621890547265
17	23.71475953565506	25.42841348811498	24.76506357103372	26.09176340519624
18	23.16196793808734	24.875621890547265	25.538971807628524	26.423438363736874
19	24.654505251520177	25.37313432835821	26.14704256495301	23.825317855168603
20	28.192371475953564	25.649530127142068	22.664455500276397	23.49364289662797
21	28.634604754007736	24.101713653952462	24.378109452736318	22.885572139303484
22	26.31288004422333	25.31785516860144	22.71973466003317	25.649530127142068
23	25.649530127142068	23.659480375898287	25.207296849087896	25.483692647871752
24	26.478717523493643	25.925925925925924	23.77003869541183	23.825317855168603
25	28.247650635710336	24.70978441127695	23.106688778330568	23.935876174682143
26	24.875621890547265	26.69983416252073	24.101713653952462	24.322830292979546
27	27.69485903814262	23.991155334438915	22.885572139303484	25.42841348811498
28	25.538971807628524	25.04145936981758	24.101713653952462	25.31785516860144
29	24.599226091763406	25.31785516860144	25.26257600884467	24.820342730790493
30	23.272526257600884	24.930901050304037	28.468767274737424	23.327805417357656
31	27.363184079601986	24.156992813709234	23.383084577114428	25.096738529574353
32	25.31785516860144	25.42841348811498	23.77003869541183	25.483692647871752
33	22.940851299060256	25.76008844665561	24.98618021006081	26.31288004422333
34	26.589275843007186	23.327805417357656	24.70978441127695	25.37313432835821
35	26.589275843007186	24.488667772249862	24.488667772249862	24.43338861249309
36	24.378109452736318	25.152017689331124	24.543946932006634	25.925925925925924
37	26.533996683250415	26.755113322277502	23.88059701492537	22.83029297954671
38	26.423438363736874	23.991155334438915	24.654505251520177	24.930901050304037
39	23.383084577114428	27.08678828081813	24.820342730790493	24.70978441127695
40	26.036484245439468	24.212271973466002	24.98618021006081	24.76506357103372
41	23.327805417357656	27.197346600331674	26.368159203980102	23.106688778330568
42	25.152017689331124	25.925925925925924	24.930901050304037	23.991155334438915
43	26.920950801547818	25.37313432835821	25.207296849087896	22.49861802100608
44	24.875621890547265	26.478717523493643	24.543946932006634	24.101713653952462
45	27.252625760088446	26.14704256495301	23.604201216141515	22.996130458817028
46	25.31785516860144	26.202321724709783	23.991155334438915	24.488667772249862
47	24.156992813709234	26.533996683250415	24.322830292979546	24.98618021006081
48	25.70480928689884	26.533996683250415	23.548922056384743	24.212271973466002
49	25.37313432835821	26.64455500276396	25.04145936981758	22.940851299060256
50	25.26257600884467	27.307904919845218	22.996130458817028	24.43338861249309
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	2.0
14	3.0
15	2.5
16	2.0
17	4.5
18	7.0
19	7.0
20	7.0
21	7.5
22	8.0
23	9.0
24	10.0
25	13.5
26	17.0
27	16.0
28	15.0
29	15.0
30	15.0
31	21.5
32	28.0
33	30.0
34	32.0
35	37.0
36	42.0
37	51.5
38	61.0
39	78.5
40	96.0
41	96.5
42	97.0
43	102.0
44	107.0
45	110.5
46	114.0
47	122.0
48	130.0
49	130.0
50	130.0
51	121.0
52	112.0
53	127.5
54	143.0
55	139.5
56	136.0
57	119.5
58	103.0
59	98.0
60	93.0
61	87.5
62	82.0
63	66.0
64	50.0
65	46.5
66	43.0
67	38.5
68	34.0
69	27.5
70	21.0
71	19.0
72	17.0
73	13.5
74	10.0
75	10.0
76	10.0
77	8.0
78	6.0
79	7.5
80	9.0
81	6.5
82	4.0
83	2.0
84	0.0
85	1.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	4.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1809.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.23604201216142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.83968163729392	95.13543394140409
2	1.8192154633314381	3.5378662244333885
3	0.22740193291642977	0.6633499170812603
4	0.05685048322910744	0.22111663902708678
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.05685048322910744	0.44223327805417356
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	8	0.44223327805417356	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	4	0.22111663902708678	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3	0.16583747927031509	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	3	0.16583747927031509	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	3	0.16583747927031509	No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG	3	0.16583747927031509	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG	2	0.11055831951354339	No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC	2	0.11055831951354339	No Hit
TCATTGATCTTGCCTTGAAGTTTCTCATCTTCCACAGTTGCTTTCATGTT	2	0.11055831951354339	No Hit
GTATCAACGCAGAGTACATGGGGGGTTTGGTTCGTGGAGTCCCAGCGGCA	2	0.11055831951354339	No Hit
CCCTAAAGCTGCCTCAGCTAGTCCACAGTCAGCCGCAGCCAGAAGGCGCA	2	0.11055831951354339	No Hit
GTGTGTGGAACACCCTGTTTCACCTTTATAGCCCCTGCTGGGTAAGTGCC	2	0.11055831951354339	No Hit
CACTAAAGGTTACAAAACCTTCCCAGTGCTGCCCTTCGTCTTGTTTGTTT	2	0.11055831951354339	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA	2	0.11055831951354339	No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA	2	0.11055831951354339	No Hit
CCCTATACGTCCACTTTCGTGTTGCAGAGTGCTGTGTTTTTAATAAACAG	2	0.11055831951354339	No Hit
ATGTAGACCAGGGTGGTCTTGAACTCACAGAGATCCACCTGCCTCTGCCT	2	0.11055831951354339	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	2	0.11055831951354339	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2	0.11055831951354339	No Hit
CTCCCGACGAGCCTTGAAAATCCGCTTGAAGAAATAGTTTTCACGCCAGT	2	0.11055831951354339	No Hit
TCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGT	2	0.11055831951354339	No Hit
AGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTA	2	0.11055831951354339	No Hit
AGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGC	2	0.11055831951354339	No Hit
CTTACCGCCTCGACCGTCAAGCGAACGTTCACGTGCCAATTGGTAAAGTT	2	0.11055831951354339	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	2	0.11055831951354339	No Hit
GCAGAGTACTGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCAT	2	0.11055831951354339	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2	0.11055831951354339	No Hit
TTACCACCCATTTTGAGCTGCATTCCCAAACAACTCGACTCTTCGAGAGC	2	0.11055831951354339	No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG	2	0.11055831951354339	No Hit
GCTGTAGACAGCCACTGAAGGTCACACGACCTCATCTGTCACTGGCATTG	2	0.11055831951354339	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2	0.11055831951354339	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT	2	0.11055831951354339	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	2	0.11055831951354339	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	2	0.11055831951354339	No Hit
GCACACAGGTGCCTGCCGTTTAGAAGCTGCCTCCTGGTCTCATGCTTAAA	2	0.11055831951354339	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA	2	0.11055831951354339	No Hit
CTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT	2	0.11055831951354339	No Hit
GACCCCGAGCACATCAGCCGCAACAAGGAGAAATACGTGCTGCTGGAGGA	2	0.11055831951354339	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE