FastQCFastQC Report
Thu 2 Feb 2017
SRR4064112_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064112_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013182
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82950.81870779386132No Hit
TATCAACGCAGAGTACTTTTTTTTT61490.6068998462270352No Hit
CCATAGGGTCTTCTCGTCTTATTAT53520.5282367827300525No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50830.5016867650629403No Hit
GTATTAGAGGCACTGCCTGCCCAGT50040.4938895479785468No Hit
GTACATGGGGTGGTATCAACGCAAA47020.4640824649470678No Hit
CTCTAATACTTGTAATGCTAGAGGT39520.39005825212054696No Hit
CCATTGGGATGTCCTGATCCAACAT37530.3704171609839101No Hit
GAGTACTTTTTTTTTTTTTTTTTTT34160.3371556146871934No Hit
CTTCTACACCATTGGGATGTCCTGA33170.3273844185940927No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32770.32343646057667824No Hit
GGTATCAACGCAGAGTACTTTTTTT30430.30034090617480375No Hit
GATATATTTTGATCAACGGACCAAG30250.2985643250669673No Hit
GTTATATAATTTAAGCTCCATAGGG28740.2836607835512277No Hit
GTCAGGATACCGCGGCCGTTAAACT27850.2748765769624806No Hit
GTACATGGGAGAAATCGTAAATAGA27080.26727675777895776No Hit
CCTCTAGCATTACAAGTATTAGAGG27020.2666845640763456No Hit
CCTATAACTTCTCTGTTAACCCAAC26610.2626379071084958No Hit
ATCTATAACTTTATAGATGCAACAC26510.26165091760414216No Hit
ACCTATAACTTCTCTGTTAACCCAA26000.2566172711319388No Hit
CTGTTAGTATGAGTAACAAGAATTC25700.25365630261887795No Hit
GTATCAACGCAGAGTACATGGGGTG25210.24882005404754526No Hit
ATCCTGACCGTGCAAAGGTAGCATA25110.24783306454319165No Hit
GTATCCTGACCGTGCAAAGGTAGCA24640.2431942138727297No Hit
GCCTAAAGGAAAGATCCAAAAAGAT24310.23993714850836279No Hit
GTATCAACGCAGAGTACATGGGAGA23320.23016595241526203No Hit
GATTAAAGATAAGAGACAGTTGGAC23130.22829067235699013No Hit
GATAGAAACCGACCTGGATTGCTCC22650.22355312273609282No Hit
ATCGTAAATAGATAGAAACCGACCT22120.21832207836301867No Hit
TCCCAATGGTGTAGAAGCTATTAAT22050.21763118570997117No Hit
ATAAATAATCCACCTATAACTTCTC22050.21763118570997117No Hit
CACTATAAATAATCCACCTATAACT21640.21358452874212136No Hit
GTACATGGGTGGTATCAACGCAAAA21610.21328843189081528No Hit
GTTCATGCTAGTCCCTAATTAAGGA21600.2131897329403799No Hit
TGCTAGAGGTGATGTTTTTGGTAAA21190.2091430759725301No Hit
GTTATAGATTAACCCAATTTTAAGT20180.19917448197855864No Hit
CTGTTAACCCAACACCGGAATGCCT20030.19769399772202823No Hit
GACTATAGGCAATAATCACACTATA19740.19483172815940275No Hit
GGTCAGGATACCGCGGCCGTTAAAC18890.18644231737239705No Hit
GTGTAGAAGCTATTAATGGTTCGTT18230.17992818664366322No Hit
TATCAACGCAGAGTACATGGGGTGG18160.1792372939906157No Hit
CTATAGAACTAGTACCGCAAGGGAA18050.17815160553582673No Hit
GATTAAACCTTGTACCTTTTGCATA17930.1769672181306024No Hit
AGGGATAACAGCGCAATCCTATTTA17010.16788691469054917No Hit
ACCTTTGCACGGTCAGGATACCGCG16990.16768951678967847No Hit
GGATTGCTCCGGTCTGAACTCAGAT16700.164827247227053No Hit
GACCTGGATTGCTCCGGTCTGAACT16610.16393895667313474No Hit
GTTAGTATGAGTAACAAGAATTCCA16530.16314936506965186No Hit
GTCCTGATCCAACATCGAGGTCGTA16360.16147148291225072No Hit
GCAGAGTACTTTTTTTTTTTTTTTT16050.15841181544875452No Hit
AAATATATCTGGGTCAATAAGATAT15940.15732612699396556No Hit
ATTTAAGAGTTCATATCGACAATTA15690.15485865323308154No Hit
CTTAAATAGGATTGCGCTGTTATCC15370.15170028681915No Hit
CCATTAATAGCTTCTACACCATTGG15180.1498250067608781No Hit
ATTATAACCTAGACTTACAAGTCAA15170.14972630781044274No Hit
CAGTTGGACCCTCGTTTAGCCGTTC14900.147061436148688No Hit
TATCAACGCAGAGTACATGGGAGAA14550.14360697288345034No Hit
ATTTCAGCCTCTTCACTGAAAGGTC14470.14281738127996746No Hit
CCTATAGTCTGATTAACTAACAATG14390.14202778967648458No Hit
AGCATGAACGGCTAAACGAGGGTCC13750.13571105684862145No Hit
CTATAGTATAAGTTTGAAATTTCGG13720.1354149599973154No Hit
CTATTAAAGGTTTTTTCCGTTCCAG13630.13452666944339714No Hit
GTTTAAAATTGAACTTAAATTCATT13360.1318617977816424No Hit
TACTAACAGTGTTGCATCTATAAAG13200.13028261457467663No Hit
GTATAAGTTTGAAATTTCGGTTGGG13200.13028261457467663No Hit
CTTTATTGGTGGCTGCTTTTAGGCC13130.1295917219216291No Hit
GTACATGGGAGTGGTATCAACGCAA13000.1283086355659694No Hit
GTATTGGAATTAGTGAAATTGGAGT12750.12584116180508537No Hit
CTCTTAAATAGGATTGCGCTGTTAT12670.12505157020160249No Hit
GATAAAAGGAACTCGGCAAACAAGA12590.12426197859811959No Hit
GTTATCCCTAGGGTAACTTGGTCCG12520.12357108594507206No Hit
CCTTTAGGCATTCCGGTGTTGGGTT12400.12238669853984774No Hit
GGGATGTCCTGATCCAACATCGAGG12180.12021532163026978No Hit
GGTATCAACGCAGAGTACATGGGGT12090.11932703107635154No Hit
CAATTAGGGTTTACGACCTCGATGT11990.11834004157199793No Hit
GGATACCGCGGCCGTTAAACTTTAG11850.11695825626590287No Hit
GGGCAGGCAGTGCCTCTAATACTTG11830.11676085836503215No Hit
GTTATAGGTGGATTATTTATAGTGT11660.11508297620763101No Hit
GCTATCACCAAGCTCGTTAGGCTTT11560.11409598670327739No Hit
ACCCTATGGAGCTTAAATTATATAA11450.11301029824848843No Hit
GTTTAGATTATAGCCAAAAGAGGGA11370.11222070664500554No Hit
ACGCAGAGTACATGGGGTGGTATCA11340.11192460979369946No Hit
GGTCTGAACTCAGATCACGTAGGAC11300.11152981399195801No Hit
ATGCAACACTGTTAGTATGAGTAAC11300.11152981399195801No Hit
ACGTAGGACTTTAATCGTTGAACAA11230.11083892133891048No Hit
GTTATACGCGTATGCCTGGAGAATT11220.11074022238847511No Hit
GGTATCAACGCAGAGTACATGGGAG10960.10817404967715574No Hit
AAGTTACCCTAGGGATAACAGCGCA10940.10797665177628502No Hit
CATTTACACCTACTACCCAACTATC10910.10768055492497892No Hit
CCCTAATTGTCGATATGAACTCTTA10900.10758185597454356No Hit
ACTAAAAGTATTGGAGAAAGAAATT10850.10708836122236677No Hit
CTATAAATAATCCACCTATAACTTC10840.1069896622719314No Hit
TTATATAACTTATCTATTTAATTTA10830.10689096332149603No Hit
GGTTATAATCATTCGGAGGATTTTT10520.10383129585799984No Hit
CGTTTAGCCGTTCATGCTAGTCCCT10360.10225211265103407No Hit
TGCTAGTCCCTAATTAAGGAACAAG10360.10225211265103407No Hit
CCAATAAAGAAAGCGTTCAAGCTCA10290.10156121999798655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACC350.002169044216.2862228
CGGCTTA1055.456968E-1215.38219314
GAACTTA1750.015.20047411
TTGCTAA1450.015.06943610
ATGCGTA1151.8189894E-1214.87002913
AAAGCGT1600.014.844213510
TGAAATG902.2828317E-914.77823913
CTTGCTA1350.014.7782399
CACCTTT1300.014.61656414
AAGCGTT1650.014.39438811
TGGGACG400.00526768714.25396619
CGACATG400.00527114214.25255715
AGGTTGA400.005276327514.2504448
GGCACAC400.00527978714.2490386
ACCTACT1152.5465852E-1114.0439178
CCTATAC1152.5465852E-1114.0411423
AGCGTTC1850.013.86529812
GGCTTAA551.95611E-413.82066115
AGGCACA551.9602133E-413.8172475
GCGTAGA1257.2759576E-1213.68245415
Produced by FastQC (version 0.11.5)