FastQCFastQC Report
Thu 2 Feb 2017
SRR4064112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013182
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG49240.4859936319437179No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT47860.47237317678363805No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA35870.35403313521164015No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT34480.340313981101125No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA29970.29580075445477716No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC29430.2904710111312676No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA28340.27971282553381327No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC26870.2652040798198152No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC26810.26461188611720304No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC26750.2640196924145909No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT26200.258591250140646No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT24980.24654997818753197No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC24250.23934495480575058No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA24190.23875276110313842No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC24140.23825926635096165No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC23980.23668008314399586No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG23380.23075814611787418No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA22850.22552710174480006No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA22780.2248362090917525No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG22600.22305962798391601No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT22280.21990126156998446No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC22260.21970386366911374No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC21990.217038992007359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21980.2169402930569236No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT21440.21161054973341414No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT21120.2084521833194826No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC20110.19848358932551113No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC20010.1974965998211575No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA19160.18910718903415183No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT19040.18792280162892747No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA18780.1853566289176081No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA18760.18515923101673737No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG18050.17815160553582673No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC17920.17686851918016702No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT16990.16768951678967847No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT16560.16344546192095794No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC16020.15811571859744844No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA15960.15752352489483626No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA15900.1569313311922241No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA15670.15466125533221078No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA15600.15397036267916325No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTC15600.15397036267916325No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC15350.15150288891827923No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT15290.15091069521566708No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG15110.14913411410783056No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA14940.14745623195042942No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT14880.14686403824781727No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT14860.14666664034694654No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA14130.13946161696516518No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG14050.1386720253616823No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT13810.13630325055123363No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTTACCAAAAACATC13740.1356123578981861No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA13610.1343292715425264No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT13600.13423057259209106No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAA13590.13413187364165569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.12988781877293518No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT12940.1277164418633572No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA12880.12712424816074508No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT12520.12357108594507206No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATCCT12220.12061011743201124No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG11700.11547777200937245No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT11620.11468818040588956No Hit
CAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGTA11580.11429338460414813No Hit
CTCTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGAT11490.11340509405022986No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11480.1133063950997945No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAAT11440.11291159929805307No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA11380.1123194055954409No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT11180.11034542658673369No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGT11180.11034542658673369No Hit
CTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCC11160.11014802868586294No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGAC10990.1084701465284618No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT10800.10659486647018995No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10700.10560787696583636No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC10610.1047195864119181No Hit
TGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGGTCA10580.10442348956061201No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC10570.10432479061017665No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT10570.10432479061017665No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACC10560.1042260916597413No Hit
CCCTAATTGTCGATATGAACTCTTAAATAGGATTGCGCTGTTATCCCTAG10550.10412739270930593No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA10400.10264690845277552No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA10390.10254820950234016No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA10370.10235081160146944No Hit
GATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTAATT10310.10175861789885726No Hit
CGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTA10260.10126512314668046No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGA10240.10106772524580973No Hit
AACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGACCCAGA10170.1003768325927622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAT250.00235102135.20485723
ATGCCTC353.2148944E-431.43290935
ATCGATA300.005740707329.33738123
CGCGGCT407.031755E-427.49972220
ATGCGTA1300.025.38310613
TTACCGC450.001399346224.44057816
GGTACGA551.5945463E-423.99738918
GGCTTAT651.8131568E-523.6920681
TAGGACC3350.023.6380524
TAGGGCG1550.022.71393431
CTGTAGG6750.022.4886631
AGGACCT4600.022.4747075
TGCGATA500.002578084822.00303628
TACGAGG602.8725294E-421.99977920
AGTCCCC904.0827945E-721.99869210
GGACCTG4600.021.9965236
ATAAGCC602.8754072E-421.9965213
AGTGTGC5400.021.9965218
TTCGTCA3050.021.63699244
GCGAGAA5000.021.55978420
Produced by FastQC (version 0.11.5)