FastQCFastQC Report
Thu 2 Feb 2017
SRR4064111_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064111_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351699
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29620.21913162619784432No Hit
TATCAACGCAGAGTACTTTTTTTTT18540.13716071403470745No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13720.10150188762438975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA607.662129E-817.42077415
CGAACGA1203.6379788E-1214.25283216
TTACGAC500.001504242913.2942753
GCGTTGA655.446971E-513.15513
CTAGAAC655.473524E-513.1481843
ATAACGA1550.012.87257412
TAACGAA1501.8189894E-1212.66777813
CGCATCG1405.2750693E-1112.21535713
CTAAGAC701.0950894E-412.2090283
ACCGGCG1103.803143E-812.0928658
TTAGAAC951.0448457E-611.9948343
CGAACCT2300.011.98064216
TCGGCGT1353.7107384E-1011.96401213
GTATCAA19050.011.9157091
GCGTTAT1201.00426405E-811.8720841
ACGAGAC1552.5465852E-1111.64661419
CGATAAC1657.2759576E-1211.51573510
GATAACG1701.0913936E-1111.17745111
CCGATAA1701.0913936E-1111.1774519
CGTGCTC855.3186835E-511.17703610
Produced by FastQC (version 0.11.5)