##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064111_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1351699
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209247029109292	32.0	32.0	32.0	32.0	32.0
2	30.75353832473058	32.0	32.0	32.0	32.0	32.0
3	30.779732765948633	32.0	32.0	32.0	32.0	32.0
4	30.807907677670844	32.0	32.0	32.0	32.0	32.0
5	30.694686464960025	32.0	32.0	32.0	32.0	32.0
6	34.35358093776795	36.0	36.0	36.0	32.0	36.0
7	34.28986408956432	36.0	36.0	36.0	32.0	36.0
8	34.24782218526462	36.0	36.0	36.0	32.0	36.0
9	34.405119778885684	36.0	36.0	36.0	32.0	36.0
10	34.06549091180803	36.0	36.0	36.0	32.0	36.0
11	34.38810637575377	36.0	36.0	36.0	32.0	36.0
12	34.17753138827505	36.0	36.0	36.0	32.0	36.0
13	34.27022510189029	36.0	36.0	36.0	32.0	36.0
14	34.15643497553819	36.0	36.0	36.0	32.0	36.0
15	34.093773835743015	36.0	36.0	36.0	32.0	36.0
16	34.08261750582045	36.0	36.0	36.0	32.0	36.0
17	33.995968037262735	36.0	36.0	36.0	32.0	36.0
18	34.00517866773594	36.0	36.0	36.0	32.0	36.0
19	34.024934545338866	36.0	36.0	36.0	32.0	36.0
20	34.01637272795201	36.0	36.0	36.0	32.0	36.0
21	33.99273432916648	36.0	36.0	36.0	32.0	36.0
22	33.97371530200141	36.0	36.0	36.0	32.0	36.0
23	33.90849368091565	36.0	36.0	36.0	32.0	36.0
24	33.89377812663914	36.0	36.0	36.0	32.0	36.0
25	33.48828992253453	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	17.0
5	15.0
6	59.0
7	22.0
8	75.0
9	100.0
10	127.0
11	42.0
12	76.0
13	82.0
14	114.0
15	246.0
16	423.0
17	545.0
18	724.0
19	974.0
20	1546.0
21	2164.0
22	3613.0
23	5548.0
24	8175.0
25	11670.0
26	16538.0
27	21614.0
28	29110.0
29	38934.0
30	50899.0
31	68885.0
32	96029.0
33	135657.0
34	282275.0
35	575398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.103930916987004	17.247736433029946	11.506409924318339	26.141922725664713
2	16.659132559108215	19.747253503360014	37.232499106634876	26.36111483089689
3	18.84357868414628	23.96502104803687	28.435416928688216	28.755983339128633
4	12.758055351689196	15.572397547290418	35.65610277231942	36.01344432870096
5	14.77859920566805	36.394623757746515	33.454722486933775	15.372054549651665
6	34.07190861259278	35.29956249292464	17.00432327725577	13.624205617226814
7	30.22634740641747	30.41482224757913	20.896805769535305	18.462024576468096
8	28.228780512715	32.508609020580984	19.375532397522495	19.88707806918152
9	27.570234231300173	14.112944711938802	18.431845686391345	39.88497537036969
10	16.028046861235843	26.33235175241065	31.372540310700714	26.267061075652798
11	36.82009730951695	21.09320350835377	22.633456382818867	19.45324279931041
12	24.879998815534268	23.46685716823855	28.657111976429135	22.99603203979805
13	29.333924584396893	19.427483402526487	25.239042114053216	25.9995498990234
14	24.03487809103306	19.41148773803231	25.03201896411063	31.521615206824
15	25.33811543671856	26.66563029751197	22.195548054216914	25.800706211552555
16	25.961814655236232	25.55930647477754	23.28661958275959	25.192259287226637
17	24.164273023393545	25.651465798045603	24.942034350014804	25.24222682854605
18	25.03739001412676	24.76418377228963	25.722997005837282	24.47542920774632
19	25.771921199772557	24.741755117513268	25.247657434609554	24.23866624810462
20	26.008831813483475	24.114523327893664	24.62201200676454	25.25463285185832
21	27.03481506325468	23.97273611021648	24.24223578111592	24.750213045412917
22	26.197379566064672	23.888062928323077	24.535471222020174	25.379086283592077
23	24.792788737066417	23.9692007872325	25.09466485605166	26.143345619649416
24	25.01103187248636	24.752412204064466	24.81312452244579	25.423431401003384
25	25.079001691095243	24.221163272136025	24.929068772193773	25.770766264574963
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	99.0
1	99.0
2	244.5
3	390.0
4	390.0
5	390.0
6	1059.0
7	1728.0
8	1728.0
9	1728.0
10	1881.0
11	2034.0
12	2034.0
13	2034.0
14	2619.5
15	3205.0
16	3205.0
17	3205.0
18	5512.0
19	7819.0
20	7819.0
21	7819.0
22	12819.0
23	17819.0
24	17819.0
25	17819.0
26	26430.0
27	35041.0
28	35041.0
29	35041.0
30	44252.0
31	53463.0
32	53463.0
33	53463.0
34	67504.0
35	81545.0
36	81545.0
37	81545.0
38	95329.5
39	109114.0
40	109114.0
41	109114.0
42	125555.0
43	141996.0
44	141996.0
45	141996.0
46	159139.0
47	176282.0
48	176282.0
49	176282.0
50	181459.5
51	186637.0
52	186637.0
53	186637.0
54	174915.5
55	163194.0
56	163194.0
57	163194.0
58	149609.5
59	136025.0
60	136025.0
61	136025.0
62	119192.5
63	102360.0
64	102360.0
65	102360.0
66	83377.5
67	64395.0
68	64395.0
69	64395.0
70	48691.0
71	32987.0
72	32987.0
73	32987.0
74	25164.5
75	17342.0
76	17342.0
77	17342.0
78	13704.0
79	10066.0
80	10066.0
81	10066.0
82	7006.0
83	3946.0
84	3946.0
85	3946.0
86	3020.5
87	2095.0
88	2095.0
89	2095.0
90	1533.5
91	972.0
92	972.0
93	972.0
94	671.5
95	371.0
96	371.0
97	371.0
98	572.5
99	774.0
100	774.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037952236407661766
2	0.005030705800625731
3	0.002145448062031562
4	0.0037730293504692983
5	0.009099659021720073
6	0.013168612242814414
7	0.024043814488284743
8	0.036990483828130374
9	0.05060298187688236
10	0.06044245057516503
11	0.055633687677508085
12	0.06502927056985319
13	0.06591704218172834
14	0.06932016669391633
15	0.061330222187040165
16	0.06798850927610363
17	0.06650888992297842
18	0.07923361635985526
19	0.07716214926547997
20	0.08337655054860588
21	0.07753205410376127
22	0.08012138797173039
23	0.08470820796641856
24	0.07893769248923023
25	0.08100915958360552
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1351699.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.956004352430085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.170597124608	43.50899680164446
2	12.068736541034852	13.264990757548867
3	3.6173526433829375	5.963857428420816
4	1.569578058036978	3.450309543558357
5	0.872409431938388	2.3972068269353555
6	0.5423768809749224	1.7884119738909174
7	0.39367758697954935	1.51444630284516
8	0.29581020045335504	1.3005237330886237
9	0.21379616669725202	1.0574444760792345
>10	1.1009423796098634	10.713945540756075
>50	0.07505543885765745	2.8875660829828935
>100	0.07114625377560457	8.248270786736516
>500	0.006221896951370403	2.3068421987822476
>1k	0.0022993966994194967	1.5971875467304941
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2962	0.21913162619784432	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1854	0.13716071403470745	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1372	0.10150188762438975	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.398096765626075E-5
2	0.0	0.0	0.0	0.0	7.398096765626075E-5
3	0.0	0.0	0.0	0.0	7.398096765626075E-5
4	0.0	0.0	0.0	0.0	7.398096765626075E-5
5	7.398096765626075E-5	0.0	0.0	0.0	7.398096765626075E-5
6	2.2194290296878225E-4	0.0	0.0	0.0	7.398096765626075E-5
7	2.2194290296878225E-4	0.0	0.0	0.0	7.398096765626075E-5
8	2.2194290296878225E-4	0.0	0.0	0.0	7.398096765626075E-5
9	2.2194290296878225E-4	0.0	0.0	7.398096765626075E-5	1.479619353125215E-4
10	2.2194290296878225E-4	0.0	0.0	7.398096765626075E-5	1.479619353125215E-4
11	2.2194290296878225E-4	0.0	0.0	7.398096765626075E-5	1.479619353125215E-4
12	2.2194290296878225E-4	0.0	0.0	7.398096765626075E-5	1.479619353125215E-4
13	2.2194290296878225E-4	0.0	0.0	7.398096765626075E-5	1.479619353125215E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	60	7.662129E-8	17.420774	15
CGAACGA	120	3.6379788E-12	14.252832	16
TTACGAC	50	0.0015042429	13.294275	3
GCGTTGA	65	5.446971E-5	13.155	13
CTAGAAC	65	5.473524E-5	13.148184	3
ATAACGA	155	0.0	12.872574	12
TAACGAA	150	1.8189894E-12	12.667778	13
CGCATCG	140	5.2750693E-11	12.215357	13
CTAAGAC	70	1.0950894E-4	12.209028	3
ACCGGCG	110	3.803143E-8	12.092865	8
TTAGAAC	95	1.0448457E-6	11.994834	3
CGAACCT	230	0.0	11.980642	16
TCGGCGT	135	3.7107384E-10	11.964012	13
GTATCAA	1905	0.0	11.915709	1
GCGTTAT	120	1.00426405E-8	11.872084	1
ACGAGAC	155	2.5465852E-11	11.646614	19
CGATAAC	165	7.2759576E-12	11.515735	10
GATAACG	170	1.0913936E-11	11.177451	11
CCGATAA	170	1.0913936E-11	11.177451	9
CGTGCTC	85	5.3186835E-5	11.177036	10
>>END_MODULE