FastQCFastQC Report
Thu 2 Feb 2017
SRR4064110_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064110_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322317
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51440.38901413201221796No Hit
TATCAACGCAGAGTACTTTTTTTTT37670.2848787393643128No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34580.26151066650432536No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21990.16629900394534744No Hit
GTACATGGGGTGGTATCAACGCAAA20600.15578715239991622No Hit
GTCCTAAAGTGTGTATTTCTCATTT20080.15185466117428725No Hit
GGTATCAACGCAGAGTACTTTTTTT19720.14913216724885184No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18340.13869594053468268No Hit
CTTTAGGACGTGAAATATGGCGAGG16500.12478097158245716No Hit
CTGTAGGACGTGGAATATGGCAAGA15790.1194116085628484No Hit
GTCCTACAGTGGACATTTCTAAATT15720.11888223474401372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG508.7400884E-515.194341
TCGCGTA400.005274364314.2517039
CGCCAGT1550.014.09952718
CGGTCCA1301.8189894E-1213.88680110
AGAACCG1351.8189894E-1213.3664035
ATTACCG1600.013.0650515
GCTCTAC957.419476E-812.9951591
CGCATCG1403.6379788E-1212.89537513
TTACCGC1650.012.6696216
CGTCTTA905.3901204E-712.6691415
CTCGTCT1051.9868821E-812.66913913
CGCGGTC853.9351144E-612.29605410
GCGGTCG853.9367806E-612.29558859
GTCCTAA5000.011.9655431
CGGTCGG907.4450218E-611.61293910
GATATAC5350.011.5377581
ATCGCCA1657.2759576E-1211.51783616
GCATCGC1657.2759576E-1211.517414
ATTATAC1001.9408599E-611.3940313
TGGCGAG10900.011.33248818
Produced by FastQC (version 0.11.5)