##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064110_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1322317
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217830520215653	32.0	32.0	32.0	32.0	32.0
2	30.80363483188978	32.0	32.0	32.0	32.0	32.0
3	30.830341741049992	32.0	32.0	32.0	32.0	32.0
4	30.845029595777714	32.0	32.0	32.0	32.0	32.0
5	30.774277272393835	32.0	32.0	32.0	32.0	32.0
6	34.41989175061653	36.0	36.0	36.0	32.0	36.0
7	34.342864834982834	36.0	36.0	36.0	32.0	36.0
8	34.29291614643085	36.0	36.0	36.0	32.0	36.0
9	34.43241824766678	36.0	36.0	36.0	32.0	36.0
10	34.116112853423196	36.0	36.0	36.0	32.0	36.0
11	34.44637405402789	36.0	36.0	36.0	32.0	36.0
12	34.24426593623163	36.0	36.0	36.0	32.0	36.0
13	34.31614053211144	36.0	36.0	36.0	32.0	36.0
14	34.2255155155685	36.0	36.0	36.0	32.0	36.0
15	34.1582850405765	36.0	36.0	36.0	32.0	36.0
16	34.158617789834054	36.0	36.0	36.0	32.0	36.0
17	34.09445919548792	36.0	36.0	36.0	32.0	36.0
18	34.087702873062966	36.0	36.0	36.0	32.0	36.0
19	34.08732777389991	36.0	36.0	36.0	32.0	36.0
20	34.059708073026364	36.0	36.0	36.0	32.0	36.0
21	34.04518508043079	36.0	36.0	36.0	32.0	36.0
22	34.017073061905734	36.0	36.0	36.0	32.0	36.0
23	33.977120463549966	36.0	36.0	36.0	32.0	36.0
24	33.959921108176026	36.0	36.0	36.0	32.0	36.0
25	33.570424489740354	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	13.0
5	13.0
6	42.0
7	20.0
8	82.0
9	72.0
10	118.0
11	42.0
12	69.0
13	87.0
14	151.0
15	235.0
16	381.0
17	512.0
18	668.0
19	903.0
20	1425.0
21	2055.0
22	3201.0
23	4900.0
24	7293.0
25	10891.0
26	15338.0
27	20261.0
28	27411.0
29	37102.0
30	48426.0
31	65849.0
32	91893.0
33	130306.0
34	275334.0
35	577220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.001316378778164	17.70620241366371	11.754052025710239	25.538429181847892
2	15.338185167846602	20.486912828067936	39.809445875928056	24.365456128157405
3	18.613031595091563	25.213606051301753	29.36661589631353	26.80674645729316
4	11.915369588744179	16.34011890185568	37.44016904301476	34.304342466385386
5	13.389865632426105	37.9574404361236	34.47897963536364	14.173714296086656
6	32.011768754561714	36.99488331461375	18.063449924176243	12.929898006648289
7	28.67119854830523	31.376863501207602	21.649252935423334	18.30268501506383
8	26.910326901021886	34.65784783352044	20.062063685632754	18.369761579824914
9	27.688641058039327	14.117531342447926	18.99817657695829	39.19565102255446
10	15.29547342789734	27.762804050321815	33.233099319447874	23.708623202332976
11	35.87255563660136	21.27908947557982	24.294195270624055	18.55415961719476
12	24.580531262746	24.102738342162993	30.62392309304703	20.692807302043974
13	29.76512626790044	20.61444929760359	25.748859608498375	23.871564825997595
14	22.51847423444967	20.83363918770125	26.80865876868707	29.83922780916201
15	23.878311114205157	29.251687811285976	23.350439716269122	23.519561358239745
16	23.66633344709717	26.37320642233424	26.443356583028805	23.51710354753979
17	22.314826936217518	26.94439000323116	26.901635783995093	23.839147276556236
18	22.739433661672827	26.016900086657408	28.765567114081257	22.478099137588504
19	24.77768881125761	25.277406523348162	26.635794106918247	23.30911055847598
20	24.915270144059676	25.602645357681848	26.670264248163495	22.811820250094986
21	25.427850101485273	24.833695834790152	25.735560106374123	24.002893957350455
22	24.776622523472987	26.04181014844404	26.05573576446334	23.12583156361963
23	23.433125574742007	26.01137563434488	26.67991174990255	23.87558704101056
24	24.026470378998066	25.838665098544944	27.0004525756702	23.13441194678679
25	23.91868521931302	25.79443496293381	27.07341716586505	23.213462651888126
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	480.0
1	480.0
2	751.0
3	1022.0
4	1022.0
5	1022.0
6	2779.5
7	4537.0
8	4537.0
9	4537.0
10	4418.5
11	4300.0
12	4300.0
13	4300.0
14	4377.0
15	4454.0
16	4454.0
17	4454.0
18	7990.5
19	11527.0
20	11527.0
21	11527.0
22	18754.0
23	25981.0
24	25981.0
25	25981.0
26	39977.0
27	53973.0
28	53973.0
29	53973.0
30	69551.5
31	85130.0
32	85130.0
33	85130.0
34	94707.0
35	104284.0
36	104284.0
37	104284.0
38	113194.5
39	122105.0
40	122105.0
41	122105.0
42	133116.0
43	144127.0
44	144127.0
45	144127.0
46	157540.5
47	170954.0
48	170954.0
49	170954.0
50	169861.0
51	168768.0
52	168768.0
53	168768.0
54	152638.5
55	136509.0
56	136509.0
57	136509.0
58	122783.5
59	109058.0
60	109058.0
61	109058.0
62	93376.0
63	77694.0
64	77694.0
65	77694.0
66	63130.0
67	48566.0
68	48566.0
69	48566.0
70	36638.5
71	24711.0
72	24711.0
73	24711.0
74	18584.5
75	12458.0
76	12458.0
77	12458.0
78	9491.0
79	6524.0
80	6524.0
81	6524.0
82	4494.0
83	2464.0
84	2464.0
85	2464.0
86	1827.0
87	1190.0
88	1190.0
89	1190.0
90	887.5
91	585.0
92	585.0
93	585.0
94	408.5
95	232.0
96	232.0
97	232.0
98	458.0
99	684.0
100	684.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03849303911240648
2	0.0051424885258224765
3	0.0017393711190281905
4	0.0037812415631047623
5	0.009301854245237716
6	0.013007470977080384
7	0.020872453428338288
8	0.03395554923668077
9	0.04741676920133372
10	0.05618924962773676
11	0.05331550603977715
12	0.06231486095996648
13	0.0636004830914221
14	0.066701101173168
15	0.05921424287822057
16	0.0654911038729745
17	0.06125611332229715
18	0.07728857754986135
19	0.07343171115549448
20	0.08008669630655886
21	0.07131421588015582
22	0.07645670440597829
23	0.0810698191129661
24	0.07494420778073639
25	0.07804482586248229
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1322317.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.2840249232459
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47447822934875	39.682999541183534
2	14.975421498059452	15.959014646774246
3	4.898691926711969	7.830660681426722
4	2.0990355664698974	4.473802537542464
5	1.0943391958584923	2.9155398493304387
6	0.5821673030126742	1.8612130249935708
7	0.3724716708068506	1.389275285333269
8	0.25527347960625907	1.0881598759666888
9	0.1786277648254596	0.8566205645649149
>10	0.8999527424220731	8.791953921331107
>50	0.0875435716141779	3.262483158244472
>100	0.0747242894723316	7.669909757717646
>500	0.005133714205801619	1.8112475302223687
>1k	0.001996444413367296	2.0162542775706065
>5k	1.4260317238337829E-4	0.39086534779812326
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5144	0.38901413201221796	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3767	0.2848787393643128	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3458	0.26151066650432536	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2199	0.16629900394534744	No Hit
GTACATGGGGTGGTATCAACGCAAA	2060	0.15578715239991622	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2008	0.15185466117428725	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1972	0.14913216724885184	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1834	0.13869594053468268	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1650	0.12478097158245716	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1579	0.1194116085628484	No Hit
GTCCTACAGTGGACATTTCTAAATT	1572	0.11888223474401372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.562483126209524E-5	0.0
5	0.0	0.0	0.0	7.562483126209524E-5	0.0
6	0.0	0.0	0.0	7.562483126209524E-5	0.0
7	0.0	0.0	0.0	7.562483126209524E-5	0.0
8	0.0	0.0	0.0	7.562483126209524E-5	0.0
9	0.0	0.0	0.0	1.5124966252419049E-4	0.0
10	0.0	0.0	0.0	1.5124966252419049E-4	0.0
11	0.0	0.0	0.0	3.0249932504838097E-4	0.0
12	0.0	0.0	0.0	3.0249932504838097E-4	1.5124966252419049E-4
13	0.0	0.0	0.0	3.0249932504838097E-4	2.2687449378628574E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	50	8.7400884E-5	15.19434	1
TCGCGTA	40	0.0052743643	14.251703	9
CGCCAGT	155	0.0	14.099527	18
CGGTCCA	130	1.8189894E-12	13.886801	10
AGAACCG	135	1.8189894E-12	13.366403	5
ATTACCG	160	0.0	13.06505	15
GCTCTAC	95	7.419476E-8	12.995159	1
CGCATCG	140	3.6379788E-12	12.895375	13
TTACCGC	165	0.0	12.66962	16
CGTCTTA	90	5.3901204E-7	12.66914	15
CTCGTCT	105	1.9868821E-8	12.669139	13
CGCGGTC	85	3.9351144E-6	12.296054	10
GCGGTCG	85	3.9367806E-6	12.2955885	9
GTCCTAA	500	0.0	11.965543	1
CGGTCGG	90	7.4450218E-6	11.612939	10
GATATAC	535	0.0	11.537758	1
ATCGCCA	165	7.2759576E-12	11.517836	16
GCATCGC	165	7.2759576E-12	11.5174	14
ATTATAC	100	1.9408599E-6	11.394031	3
TGGCGAG	1090	0.0	11.332488	18
>>END_MODULE