FastQCFastQC Report
Thu 2 Feb 2017
SRR4064110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322317
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16440.12432722259488459No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13910.10519414028557449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1950.021.4341495
TAGGACC7550.020.6870524
TTAACGG1502.1827873E-1120.5355435
TGTAGGA12350.020.3068032
TTAGGAC9000.020.2883
GTCGTAG2300.020.08455711
CGTCGTA2300.020.08455710
TAGGACG12450.019.7895494
GATATAC5350.019.7389471
CCGTCGT2250.019.5539599
TACCGTC2250.019.5539597
GTCCTAC11950.019.5152651
GACGGTA2850.019.2966698
TACGACG2750.019.1984335
ATACCGT2300.019.1288726
GTCCTAA8150.018.8963071
CGACGGT2800.018.8556027
ACCGTCG2350.018.7218768
GTAGGAC12850.018.6606623
ACGGTAT2950.018.6425469
Produced by FastQC (version 0.11.5)