##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1322317
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276395902041642	32.0	32.0	32.0	32.0	32.0
2	31.441185434355	32.0	32.0	32.0	32.0	32.0
3	31.50287487796043	32.0	32.0	32.0	32.0	32.0
4	31.585767255506813	32.0	32.0	32.0	32.0	32.0
5	31.54193132206574	32.0	32.0	32.0	32.0	32.0
6	35.12515304575226	36.0	36.0	36.0	36.0	36.0
7	35.14828743788365	36.0	36.0	36.0	36.0	36.0
8	35.10716189839501	36.0	36.0	36.0	36.0	36.0
9	35.19590688163277	36.0	36.0	36.0	36.0	36.0
10	35.06885943385739	36.0	36.0	36.0	36.0	36.0
11	35.20338542119628	36.0	36.0	36.0	36.0	36.0
12	35.11494218103526	36.0	36.0	36.0	36.0	36.0
13	35.15314255205068	36.0	36.0	36.0	36.0	36.0
14	35.094796482235346	36.0	36.0	36.0	36.0	36.0
15	35.07772342032962	36.0	36.0	36.0	36.0	36.0
16	35.08868221462781	36.0	36.0	36.0	36.0	36.0
17	35.06278222241717	36.0	36.0	36.0	36.0	36.0
18	35.0482297361374	36.0	36.0	36.0	36.0	36.0
19	35.04495064345387	36.0	36.0	36.0	36.0	36.0
20	35.03475187870987	36.0	36.0	36.0	36.0	36.0
21	35.029408984381206	36.0	36.0	36.0	36.0	36.0
22	35.01578592727765	36.0	36.0	36.0	36.0	36.0
23	34.97441460708741	36.0	36.0	36.0	36.0	36.0
24	34.953756171931545	36.0	36.0	36.0	36.0	36.0
25	34.919481485906935	36.0	36.0	36.0	36.0	36.0
26	34.873468313573824	36.0	36.0	36.0	32.0	36.0
27	34.85309800902507	36.0	36.0	36.0	32.0	36.0
28	34.823788093172816	36.0	36.0	36.0	32.0	36.0
29	34.793944266011856	36.0	36.0	36.0	32.0	36.0
30	34.771824002867696	36.0	36.0	36.0	32.0	36.0
31	34.76146037599153	36.0	36.0	36.0	32.0	36.0
32	34.705696137915496	36.0	36.0	36.0	32.0	36.0
33	34.68608283792767	36.0	36.0	36.0	32.0	36.0
34	34.65580492423526	36.0	36.0	36.0	32.0	36.0
35	34.622150361827	36.0	36.0	36.0	32.0	36.0
36	34.586841884359046	36.0	36.0	36.0	32.0	36.0
37	34.549366755475425	36.0	36.0	36.0	32.0	36.0
38	34.51273181846713	36.0	36.0	36.0	32.0	36.0
39	34.4821824116305	36.0	36.0	36.0	32.0	36.0
40	34.46000694235951	36.0	36.0	36.0	32.0	36.0
41	34.444707282746876	36.0	36.0	36.0	32.0	36.0
42	34.38023106410944	36.0	36.0	36.0	32.0	36.0
43	34.368987920445704	36.0	36.0	36.0	32.0	36.0
44	34.2903365834365	36.0	36.0	36.0	32.0	36.0
45	34.24442701712222	36.0	36.0	36.0	32.0	36.0
46	34.175277940161095	36.0	36.0	36.0	32.0	36.0
47	34.13843730361177	36.0	36.0	36.0	32.0	36.0
48	34.06574217831277	36.0	36.0	36.0	32.0	36.0
49	34.03896418181117	36.0	36.0	36.0	32.0	36.0
50	33.47657256164747	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	3.0
20	14.0
21	43.0
22	140.0
23	436.0
24	1179.0
25	2598.0
26	5271.0
27	9611.0
28	16037.0
29	24249.0
30	33996.0
31	46907.0
32	67316.0
33	106172.0
34	218582.0
35	789758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18725159865527	18.594922875045853	12.330463127881078	25.887362398417796
2	14.7642495059778	21.23482484048736	39.14924575118221	24.85167990235263
3	17.900705658502424	25.717323685577746	29.69883786794483	26.683132787974994
4	11.560087331555142	17.047803212089082	37.47989324798819	33.91221620836758
5	12.976086672106613	38.23190657005846	34.44998438347234	14.342022374362578
6	32.09333630791453	37.37301626314456	17.689733535504022	12.84391389343689
7	28.444767782611883	31.602860736116984	21.686554736874744	18.26581674439639
8	26.807338936124996	34.944797654420235	19.936369266976072	18.311494142478697
9	27.494840425652594	14.008026928856484	19.018088949624858	39.47904369586606
10	15.116406577907519	27.96798015586419	33.29398285569517	23.621630410533122
11	35.87077521560656	21.237434924783486	24.23165113331458	18.66013872629538
12	24.477544667907924	24.16430590616147	30.69573490109006	20.662414524840543
13	29.83997700992959	20.545862921705197	25.783212711088925	23.83094735727628
14	22.58602917766066	20.754314598737214	26.868071326531616	29.79158489707051
15	23.8975840514878	29.33624754680451	23.450922855273756	23.315245546433932
16	23.685836474943375	26.462881105342568	26.317301984035453	23.533980435678608
17	22.342751397735945	26.97416731388918	26.951479864510553	23.731601423864323
18	22.867505651082613	25.803270481345947	28.9572136097883	22.372010257783142
19	24.77562095595909	25.107084842049858	26.924804660913125	23.192489541077926
20	24.899247599449748	25.53957079363927	26.848349959653667	22.71283164725732
21	25.55037861571771	24.736731056168832	25.941434618174007	23.77145570993945
22	24.846159721639278	25.858854900466078	26.314495887131113	22.980489490763535
23	23.416085045174768	25.82981738761515	26.968885487431454	23.78521207977863
24	24.152031260516658	25.66930372447548	27.111555788481795	23.067109226526064
25	23.901694564039424	25.6193247003109	27.281218071417175	23.1977626642325
26	22.788873065488456	26.567178401991175	27.860898948974956	22.783049583545413
27	23.392708043417755	26.30764530064497	26.898165515071575	23.401481140865695
28	22.890296076638542	26.422811264661462	26.974972376818762	23.711920281881234
29	23.41622380351321	25.63914852295338	26.6449211846019	24.29970648893151
30	22.963666587508904	26.157984774483573	27.086928835155728	23.791419802851795
31	24.027220947847443	25.503680560755154	26.677844862076345	23.79125362932106
32	23.259714929413	26.49740710853843	26.560177997064137	23.682699964984433
33	22.93943567276008	26.169921353574633	27.33089959742771	23.55974337623757
34	23.655897714364368	26.17068680998716	27.318571715958612	22.854843759689864
35	23.736025053751973	26.36648750602181	27.07942435662119	22.818063083605022
36	23.00652774195793	26.471502484868058	26.953349826010076	23.56861994716394
37	23.648400814661485	26.41811373950949	26.14358522144166	23.78990022438736
38	23.366447460472926	26.888393920715174	26.369408445804137	23.375750173007763
39	24.029268292682925	25.968084704102857	26.296691245982228	23.70595575723199
40	24.63120128627674	26.173175442762652	26.602211657866114	22.593411613094492
41	23.56358262595217	25.9865017757637	27.417700764153167	23.032214834130965
42	23.915361601592398	27.07345774432724	26.62945327932577	22.381727374754593
43	22.97684112770088	26.509092861610178	27.054581369899893	23.45948464078905
44	22.73988163031329	26.90906285875045	26.337104041600305	24.013951469335957
45	22.954629502885524	26.99367990858291	26.464598186027132	23.587092402504442
46	23.225755221036152	26.142199736064946	26.19581863351219	24.43622640938671
47	23.913229256236722	26.291604936591018	26.365870093451584	23.429295713720677
48	23.44209902200119	26.659467046613	25.890801729118834	24.007632202266972
49	23.566107796205127	26.620005898768067	26.049337901097324	23.764548403929485
50	23.125096895927157	27.50430123383965	25.63293364945039	23.73766822078281
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	212.0
1	143.5
2	75.0
3	217.5
4	360.0
5	377.5
6	395.0
7	383.5
8	372.0
9	537.5
10	703.0
11	1032.5
12	1362.0
13	2490.5
14	3619.0
15	4867.0
16	6115.0
17	6528.5
18	6942.0
19	6940.0
20	6938.0
21	7460.5
22	7983.0
23	9114.0
24	10245.0
25	11298.5
26	12352.0
27	15945.5
28	19539.0
29	23699.5
30	27860.0
31	31534.5
32	35209.0
33	41714.0
34	48219.0
35	55536.0
36	62853.0
37	66447.0
38	70041.0
39	69545.0
40	69049.0
41	70902.5
42	72756.0
43	72624.5
44	72493.0
45	77173.0
46	81853.0
47	84694.0
48	87535.0
49	88677.0
50	89819.0
51	88189.0
52	86559.0
53	84353.0
54	82147.0
55	80962.0
56	79777.0
57	75961.5
58	72146.0
59	66068.0
60	59990.0
61	52701.5
62	45413.0
63	39037.0
64	32661.0
65	28028.5
66	23396.0
67	19629.5
68	15863.0
69	13760.5
70	11658.0
71	9007.0
72	6356.0
73	5427.0
74	4498.0
75	3506.0
76	2514.0
77	2133.0
78	1752.0
79	1458.0
80	1164.0
81	907.0
82	650.0
83	545.0
84	440.0
85	323.0
86	206.0
87	139.0
88	72.0
89	62.5
90	53.0
91	41.0
92	29.0
93	26.0
94	23.0
95	22.5
96	22.0
97	19.5
98	17.0
99	14.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008469981101354667
2	6.049986500967619E-4
3	4.537489875725715E-4
4	0.0
5	0.0
6	1.5124966252419049E-4
7	0.0
8	0.0
9	0.0013612469627177143
10	9.07497975145143E-4
11	7.562483126209524E-5
12	2.2687449378628574E-4
13	5.293738188346667E-4
14	3.0249932504838097E-4
15	0.0046887395382499055
16	9.07497975145143E-4
17	0.0
18	3.0249932504838097E-4
19	7.562483126209524E-5
20	0.0012099973001935239
21	0.0
22	3.0249932504838097E-4
23	6.049986500967619E-4
24	7.562483126209525E-4
25	0.0022687449378628577
26	0.006352485826016
27	0.007033109307374858
28	0.004386240213201525
29	0.0037812415631047623
30	0.004764364369512
31	0.007562483126209525
32	0.003629991900580572
33	0.005293738188346667
34	0.004461865044463619
35	0.00302499325048381
36	0.008621230763878858
37	0.0037812415631047623
38	0.00922622941397562
39	0.005066863694560381
40	0.0038568663943668573
41	0.005218113357084572
42	0.002495619431649143
43	0.0012099973001935239
44	0.0014368717939798096
45	0.0019662456128144764
46	0.0016637462877660955
47	0.002117495275338667
48	0.0018906207815523812
49	5.293738188346667E-4
50	0.0016637462877660955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1322317.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.02624630401609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6610338787399	43.90325787464267
2	14.39982562022816	16.71135656348481
3	4.808275407560006	8.370185192898608
4	1.9710113922219958	4.574815700523808
5	0.991378086313415	2.876297450841317
6	0.542905967028216	1.8901677217619581
7	0.3317367746241397	1.3474607854708143
8	0.22082756934009326	1.0251035943396354
9	0.17700365930072468	0.92437721381664
>10	0.7700043384520957	8.01886093453715
>50	0.06719793147767598	2.7999282878050504
>100	0.05459944481550804	5.825666308995604
>500	0.003937434279436942	1.5013823033788667
>1k	2.624956186291295E-4	0.23114006750303645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1644	0.12432722259488459	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1391	0.10519414028557449	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	7.562483126209524E-5	0.0
7	0.0	0.0	0.0	7.562483126209524E-5	0.0
8	0.0	0.0	0.0	7.562483126209524E-5	0.0
9	0.0	0.0	0.0	2.2687449378628574E-4	0.0
10	0.0	0.0	0.0	2.2687449378628574E-4	0.0
11	0.0	0.0	0.0	2.2687449378628574E-4	0.0
12	0.0	0.0	0.0	3.0249932504838097E-4	2.2687449378628574E-4
13	0.0	0.0	0.0	3.0249932504838097E-4	2.2687449378628574E-4
14	0.0	0.0	0.0	3.0249932504838097E-4	2.2687449378628574E-4
15	0.0	0.0	0.0	3.0249932504838097E-4	2.2687449378628574E-4
16	0.0	0.0	0.0	3.0249932504838097E-4	2.2687449378628574E-4
17	0.0	0.0	0.0	3.0249932504838097E-4	2.2687449378628574E-4
18	0.0	0.0	0.0	3.0249932504838097E-4	2.2687449378628574E-4
19	0.0	0.0	0.0	4.537489875725715E-4	2.2687449378628574E-4
20	0.0	0.0	0.0	5.293738188346667E-4	2.2687449378628574E-4
21	0.0	0.0	0.0	7.562483126209525E-4	3.0249932504838097E-4
22	0.0	0.0	0.0	0.0012856221314556191	3.0249932504838097E-4
23	0.0	0.0	0.0	0.0026468690941733337	3.0249932504838097E-4
24	0.0	0.0	0.0	0.00461311470698781	3.0249932504838097E-4
25	0.0	0.0	0.0	0.005218113357084572	3.0249932504838097E-4
26	0.0	0.0	0.0	0.006352485826016001	3.0249932504838097E-4
27	0.0	0.0	0.0	0.008016232113782096	3.0249932504838097E-4
28	0.0	0.0	0.0	0.012553721989507811	3.0249932504838097E-4
29	0.0	0.0	0.0	0.01920870714057219	3.0249932504838097E-4
30	0.0	0.0	0.0	0.034031174067942864	3.0249932504838097E-4
31	0.0	0.0	0.0	0.06496173005413981	3.0249932504838097E-4
32	0.0	0.0	0.0	0.1002785262535383	3.0249932504838097E-4
33	0.0	0.0	0.0	0.13007470977080382	3.0249932504838097E-4
34	0.0	0.0	0.0	0.16629900394534744	3.0249932504838097E-4
35	0.0	0.0	0.0	0.20585079069542325	3.0249932504838097E-4
36	0.0	0.0	0.0	0.2603762940353939	3.0249932504838097E-4
37	0.0	0.0	0.0	0.34621047751787204	3.0249932504838097E-4
38	0.0	0.0	0.0	0.46199209418013987	3.7812415631047625E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	195	0.0	21.434149	5
TAGGACC	755	0.0	20.687052	4
TTAACGG	150	2.1827873E-11	20.53554	35
TGTAGGA	1235	0.0	20.306803	2
TTAGGAC	900	0.0	20.288	3
GTCGTAG	230	0.0	20.084557	11
CGTCGTA	230	0.0	20.084557	10
TAGGACG	1245	0.0	19.789549	4
GATATAC	535	0.0	19.738947	1
CCGTCGT	225	0.0	19.553959	9
TACCGTC	225	0.0	19.553959	7
GTCCTAC	1195	0.0	19.515265	1
GACGGTA	285	0.0	19.296669	8
TACGACG	275	0.0	19.198433	5
ATACCGT	230	0.0	19.128872	6
GTCCTAA	815	0.0	18.896307	1
CGACGGT	280	0.0	18.855602	7
ACCGTCG	235	0.0	18.721876	8
GTAGGAC	1285	0.0	18.660662	3
ACGGTAT	295	0.0	18.642546	9
>>END_MODULE