FastQCFastQC Report
Thu 2 Feb 2017
SRR4064109_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064109_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14946
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACTTTTTTTTTTTTTT4543.0376020339890273No Hit
ACGCAGAGTACATGGGGAATAATTG800.5352602703064365No Hit
GTATCAACGCAGAGTACTTTTTTTT750.5018065034122842No Hit
ACGCAGAGTACATGGGAATAACGCC730.4884249966546233No Hit
TATCAACGCAGAGTACTTTTTTTTT500.3345376689415228No Hit
ACGCAGAGTACATGGGTACCTGGTT450.3010839020473705No Hit
GGTATCAACGCAGAGTACTTTTTTT380.25424862839555734No Hit
CCGCAGCTAGGAATAATGGAATAGG310.20741335474374414No Hit
GCGCAAGACGGACCAGAGCGAAAGC280.18734109460725276No Hit
ACGCAGAGTACATGGGAGAAATCGT250.1672688344707614No Hit
GTACTTTTTTTTTTTTTTTTTTTTT220.14719657433427003No Hit
CTATTGGAGCTGGAATTACCGCGGC200.13381506757660913No Hit
GAATAGGACCGCGGTTCTATTTTGT190.12712431419777867No Hit
GGGTAGGCACACGCTGAGCCAGTCA180.12043356081894822No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG170.11374280744011775No Hit
GTATCTGATCGTCTTCGAACCTCCG160.1070520540612873No Hit
AAGCAGGCCCGAGCCGCCTGGATAC160.1070520540612873No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG160.1070520540612873No Hit
GAGTACTTTTTTTTTTTTTTTTTTT150.10036130068245684No Hit
GGCTCCACTCCTGGTGGTGCCCTTC150.10036130068245684No Hit
GAACTACGACGGTATCTGATCGTCT150.10036130068245684No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT150.10036130068245684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCT250.005702635719.03366912
CATGGGC503.914751E-617.13030211
CATGGGA402.5033846E-416.6544611
ACGCAGA2000.016.5985741
CGCAGAG2000.016.5985742
AGTACAT1500.016.4958487
GAGTACA1500.016.4958486
GCAGAGT2150.015.8816923
CAGAGTA2100.015.8613924
AGAGTAC2050.015.78401855
ACATGGG1550.014.73574410
TACATGG1600.014.2752529
GTACATG1650.013.84266958
GTACTTT657.127821E-411.7130288
ACTTTTT657.127821E-411.71302810
AGTACTT657.127821E-411.7130287
GAGTACT657.127821E-411.7130286
TACTTTT657.127821E-411.7130289
CTTTTTT700.001326764110.87638311
Produced by FastQC (version 0.11.5)