##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064109_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14946
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.90090994245952	32.0	32.0	32.0	14.0	32.0
2	27.48186805834337	32.0	21.0	32.0	14.0	32.0
3	28.00086979793925	32.0	21.0	32.0	14.0	32.0
4	27.46447209955841	32.0	21.0	32.0	14.0	32.0
5	27.5486417770641	32.0	21.0	32.0	14.0	32.0
6	31.34852134350328	36.0	32.0	36.0	14.0	36.0
7	30.620099023150008	36.0	21.0	36.0	14.0	36.0
8	30.895289709621302	36.0	27.0	36.0	14.0	36.0
9	31.123645122440788	36.0	32.0	36.0	14.0	36.0
10	29.92941255185334	36.0	21.0	36.0	14.0	36.0
11	31.589321557607388	36.0	32.0	36.0	14.0	36.0
12	30.479593202194568	36.0	21.0	36.0	14.0	36.0
13	30.99685534591195	36.0	32.0	36.0	14.0	36.0
14	30.53954235246889	36.0	27.0	36.0	14.0	36.0
15	30.527498996386992	36.0	27.0	36.0	14.0	36.0
16	30.46206342834203	36.0	21.0	36.0	14.0	36.0
17	30.231500066907532	36.0	21.0	36.0	14.0	36.0
18	30.34089388465141	36.0	21.0	36.0	14.0	36.0
19	30.321825237521743	36.0	21.0	36.0	14.0	36.0
20	30.36658637762612	36.0	21.0	36.0	14.0	36.0
21	30.32396627860297	36.0	21.0	36.0	14.0	36.0
22	30.144654088050313	36.0	21.0	36.0	14.0	36.0
23	29.95771443864579	36.0	21.0	36.0	14.0	36.0
24	29.99799277398635	36.0	21.0	36.0	14.0	36.0
25	29.191221731566973	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	1.0
12	0.0
13	2.0
14	17.0
15	51.0
16	109.0
17	180.0
18	299.0
19	423.0
20	428.0
21	446.0
22	480.0
23	414.0
24	405.0
25	431.0
26	448.0
27	467.0
28	540.0
29	622.0
30	682.0
31	830.0
32	1137.0
33	1321.0
34	2304.0
35	2903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.384929398380514	28.033192799304025	9.877534631600081	25.704343170715383
2	13.287836210357288	17.931219055265622	32.81814532316339	35.9627994112137
3	31.12538471831928	20.446942325705876	24.387796065837016	24.03987689013783
4	10.063566410170626	13.489461358313818	30.672465707594515	45.77450652392104
5	12.151221144195382	46.05553696888591	28.390766142522583	13.402475744396119
6	42.29791220556745	31.591274089935762	14.427194860813705	11.683618843683083
7	25.62232334047109	39.67478586723769	17.913543897216275	16.789346895074946
8	35.040160642570285	29.397590361445786	17.8045515394913	17.75769745649264
9	23.716446883994912	11.6875292857621	27.893433295401298	36.70259053484169
10	13.629669299772393	35.138572767438745	27.594055429106973	23.637702503681883
11	32.18852513891679	18.31023632590212	20.70696927093794	28.794269264243155
12	21.236271095633537	29.594160192874362	29.19233860166086	19.977230109831233
13	25.43519014461703	17.474558114622386	34.5607927155865	22.529459025174077
14	28.992299966521596	17.75025108804821	25.939069300301306	27.318379645128893
15	30.19347927964116	24.563165294235787	23.264377050277833	21.97897837584522
16	30.24236743438672	22.837439742903054	25.395018746652383	21.525174076057844
17	22.42083417018143	26.149829282988552	27.662850639351944	23.766485907478074
18	22.751925008369604	25.71811181787747	29.72882490793438	21.801138265818548
19	23.280883829929696	24.71375962504185	29.487780381653835	22.517576163374624
20	22.90592567793773	23.65584198192166	29.28021426180114	24.15801807833947
21	23.937060595915636	24.07766990291262	28.175426849681955	23.80984265148979
22	23.052709128658496	24.244859687897662	29.40861295291675	23.29381823052709
23	23.0295319091944	23.210339516507066	29.920310721221455	23.83981785307708
24	22.929083238465147	23.190249782361214	29.545302350498897	24.335364628674746
25	22.937315831770693	23.81462630592017	28.890972408250736	24.357085454058396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	5.0
3	9.0
4	9.0
5	9.0
6	20.0
7	31.0
8	31.0
9	31.0
10	29.5
11	28.0
12	28.0
13	28.0
14	34.0
15	40.0
16	40.0
17	40.0
18	70.5
19	101.0
20	101.0
21	101.0
22	401.0
23	701.0
24	701.0
25	701.0
26	606.0
27	511.0
28	511.0
29	511.0
30	630.0
31	749.0
32	749.0
33	749.0
34	864.5
35	980.0
36	980.0
37	980.0
38	1060.5
39	1141.0
40	1141.0
41	1141.0
42	1310.5
43	1480.0
44	1480.0
45	1480.0
46	1669.5
47	1859.0
48	1859.0
49	1859.0
50	1990.5
51	2122.0
52	2122.0
53	2122.0
54	1856.5
55	1591.0
56	1591.0
57	1591.0
58	1415.0
59	1239.0
60	1239.0
61	1239.0
62	1070.0
63	901.0
64	901.0
65	901.0
66	783.5
67	666.0
68	666.0
69	666.0
70	505.5
71	345.0
72	345.0
73	345.0
74	270.0
75	195.0
76	195.0
77	195.0
78	168.5
79	142.0
80	142.0
81	142.0
82	98.0
83	54.0
84	54.0
85	54.0
86	39.0
87	24.0
88	24.0
89	24.0
90	17.5
91	11.0
92	11.0
93	11.0
94	7.0
95	3.0
96	3.0
97	3.0
98	12.5
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02007226013649137
2	0.0
3	0.0
4	0.006690753378830456
5	0.006690753378830456
6	0.013381506757660913
7	0.013381506757660913
8	0.04014452027298274
9	0.04683527365181319
10	0.05352602703064365
11	0.06021678040947411
12	0.09367054730362638
13	0.06690753378830457
14	0.07359828716713501
15	0.06021678040947411
16	0.06690753378830457
17	0.06021678040947411
18	0.07359828716713501
19	0.07359828716713501
20	0.07359828716713501
21	0.07359828716713501
22	0.10036130068245684
23	0.08697979392479593
24	0.08697979392479593
25	0.09367054730362638
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	14946.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.97457513716044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.37848238935833	73.8792988090459
2	4.50932820212499	7.212632142379231
3	1.246548983518782	2.990766760337214
4	0.4685016313896093	1.498728756858022
5	0.3764745252237932	1.5054195102368526
6	0.2760813184974483	1.3247691690084302
7	0.1338576089684598	0.749364378429011
8	0.11712540784740233	0.749364378429011
9	0.05019660336317242	0.3613006824568446
>10	0.4099389274659081	5.165261608457112
>50	0.02509830168158621	1.5254917703733442
>100	0.008366100560528738	3.0376020339890273
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACGCAGAGTACTTTTTTTTTTTTTT	454	3.0376020339890273	No Hit
ACGCAGAGTACATGGGGAATAATTG	80	0.5352602703064365	No Hit
GTATCAACGCAGAGTACTTTTTTTT	75	0.5018065034122842	No Hit
ACGCAGAGTACATGGGAATAACGCC	73	0.4884249966546233	No Hit
TATCAACGCAGAGTACTTTTTTTTT	50	0.3345376689415228	No Hit
ACGCAGAGTACATGGGTACCTGGTT	45	0.3010839020473705	No Hit
GGTATCAACGCAGAGTACTTTTTTT	38	0.25424862839555734	No Hit
CCGCAGCTAGGAATAATGGAATAGG	31	0.20741335474374414	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	28	0.18734109460725276	No Hit
ACGCAGAGTACATGGGAGAAATCGT	25	0.1672688344707614	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	22	0.14719657433427003	No Hit
CTATTGGAGCTGGAATTACCGCGGC	20	0.13381506757660913	No Hit
GAATAGGACCGCGGTTCTATTTTGT	19	0.12712431419777867	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	18	0.12043356081894822	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	17	0.11374280744011775	No Hit
GTATCTGATCGTCTTCGAACCTCCG	16	0.1070520540612873	No Hit
AAGCAGGCCCGAGCCGCCTGGATAC	16	0.1070520540612873	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	16	0.1070520540612873	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	15	0.10036130068245684	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	15	0.10036130068245684	No Hit
GAACTACGACGGTATCTGATCGTCT	15	0.10036130068245684	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	15	0.10036130068245684	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCT	25	0.0057026357	19.033669	12
CATGGGC	50	3.914751E-6	17.130302	11
CATGGGA	40	2.5033846E-4	16.65446	11
ACGCAGA	200	0.0	16.598574	1
CGCAGAG	200	0.0	16.598574	2
AGTACAT	150	0.0	16.495848	7
GAGTACA	150	0.0	16.495848	6
GCAGAGT	215	0.0	15.881692	3
CAGAGTA	210	0.0	15.861392	4
AGAGTAC	205	0.0	15.7840185	5
ACATGGG	155	0.0	14.735744	10
TACATGG	160	0.0	14.275252	9
GTACATG	165	0.0	13.8426695	8
GTACTTT	65	7.127821E-4	11.713028	8
ACTTTTT	65	7.127821E-4	11.713028	10
AGTACTT	65	7.127821E-4	11.713028	7
GAGTACT	65	7.127821E-4	11.713028	6
TACTTTT	65	7.127821E-4	11.713028	9
CTTTTTT	70	0.0013267641	10.876383	11
>>END_MODULE