##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14946
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.941389000401447	32.0	32.0	32.0	21.0	32.0
2	29.718854543021543	32.0	32.0	32.0	21.0	32.0
3	30.68740800214104	32.0	32.0	32.0	32.0	32.0
4	31.054195102368528	32.0	32.0	32.0	32.0	32.0
5	30.80396092600027	32.0	32.0	32.0	32.0	32.0
6	34.293991703465814	36.0	36.0	36.0	32.0	36.0
7	34.44312859627994	36.0	36.0	36.0	32.0	36.0
8	34.240331861367586	36.0	36.0	36.0	32.0	36.0
9	34.54817342432758	36.0	36.0	36.0	32.0	36.0
10	34.063896694767834	36.0	36.0	36.0	32.0	36.0
11	34.624782550515185	36.0	36.0	36.0	32.0	36.0
12	34.39723002810116	36.0	36.0	36.0	32.0	36.0
13	34.489094071992504	36.0	36.0	36.0	32.0	36.0
14	34.39562424729024	36.0	36.0	36.0	32.0	36.0
15	34.359561086578346	36.0	36.0	36.0	32.0	36.0
16	34.35340559346982	36.0	36.0	36.0	32.0	36.0
17	34.300080289040544	36.0	36.0	36.0	32.0	36.0
18	34.3621035728623	36.0	36.0	36.0	32.0	36.0
19	34.3083768232303	36.0	36.0	36.0	32.0	36.0
20	34.367924528301884	36.0	36.0	36.0	32.0	36.0
21	34.305299076676036	36.0	36.0	36.0	32.0	36.0
22	34.323631740934026	36.0	36.0	36.0	32.0	36.0
23	34.229894286096616	36.0	36.0	36.0	32.0	36.0
24	34.145925331192295	36.0	36.0	36.0	32.0	36.0
25	34.112538471831925	36.0	36.0	36.0	32.0	36.0
26	34.033587581961726	36.0	36.0	36.0	32.0	36.0
27	34.061822561220396	36.0	36.0	36.0	32.0	36.0
28	34.0137160444266	36.0	36.0	36.0	32.0	36.0
29	33.977987421383645	36.0	36.0	36.0	32.0	36.0
30	33.840693162050044	36.0	36.0	36.0	32.0	36.0
31	33.881038404924396	36.0	36.0	36.0	32.0	36.0
32	33.85380703867256	36.0	36.0	36.0	32.0	36.0
33	33.799611936304025	36.0	36.0	36.0	32.0	36.0
34	33.76856684062626	36.0	36.0	36.0	32.0	36.0
35	33.720661046433825	36.0	36.0	36.0	32.0	36.0
36	33.67998126589054	36.0	36.0	36.0	27.0	36.0
37	33.70058878629734	36.0	36.0	36.0	32.0	36.0
38	33.559547705071594	36.0	36.0	36.0	27.0	36.0
39	33.634818680583436	36.0	36.0	36.0	27.0	36.0
40	33.546300013381504	36.0	36.0	36.0	21.0	36.0
41	33.58263080422856	36.0	36.0	36.0	27.0	36.0
42	33.431085240198044	36.0	36.0	36.0	21.0	36.0
43	33.451224407868324	36.0	36.0	36.0	21.0	36.0
44	33.28683259735046	36.0	36.0	36.0	21.0	36.0
45	33.322962665596144	36.0	36.0	36.0	21.0	36.0
46	33.277197912484944	36.0	36.0	36.0	21.0	36.0
47	33.23651813194166	36.0	36.0	36.0	21.0	36.0
48	33.130268968285826	36.0	36.0	36.0	14.0	36.0
49	33.17402649538338	36.0	36.0	36.0	21.0	36.0
50	32.30329185066238	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	1.0
23	7.0
24	16.0
25	57.0
26	102.0
27	174.0
28	318.0
29	500.0
30	695.0
31	1061.0
32	1525.0
33	2192.0
34	3475.0
35	4821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9277350284376	18.447641351622615	13.101371696219472	24.523251923720306
2	17.14171015656363	21.336812525090327	37.35447611401044	24.16700120433561
3	20.19938445068915	24.1870734644721	29.19844774521611	26.41509433962264
4	13.394888264418572	16.7402649538338	36.70547303626389	33.15937374548374
5	17.02127659574468	35.00602167804095	31.613809714973907	16.358892011240467
6	34.2700388063696	36.69209152950622	15.96413756188947	13.073732102234711
7	30.001338150675767	30.18198849190419	19.97858958918774	19.838083768232302
8	27.70640974173692	33.54074668807708	18.35942727151077	20.39341629867523
9	26.756322761942997	13.227619429947811	17.489629332262812	42.52642847584638
10	14.438645791516125	26.716178241670015	31.98849190418841	26.85668406262545
11	38.652482269503544	20.19938445068915	22.2534457379901	18.89468754181721
12	24.140238190820288	23.397564565770107	29.619965208082434	22.84223203532718
13	29.9210491101298	18.707346447209954	25.05018065034123	26.321423792319017
14	23.103171417101564	20.10571390338552	24.996654623310587	31.79446005620233
15	25.27266644362663	27.280026764804283	21.75978588156574	25.687520910003347
16	24.46303111408498	26.77818668450987	24.041485446637672	24.71729675476748
17	22.74856148802355	26.69610598153352	24.836076542218656	25.719255988224276
18	24.106784423926133	25.68580222133012	26.24113475177305	23.966278602970696
19	25.545296400374685	25.47838886658638	24.862839555733977	24.113475177304963
20	25.404790579419245	24.581827913823094	24.76247825505152	25.25090325170614
21	27.030643650475046	24.675498461126725	23.257058744814664	25.036799143583565
22	25.177304964539005	25.39140907266158	24.849458048976313	24.581827913823094
23	23.43101833266426	25.431553592934563	25.47838886658638	25.6590392078148
24	23.544761140104377	27.151077211293988	24.708952228020877	24.59520942058076
25	24.382736701237874	25.600535296085646	25.245901639344265	24.77082636333222
26	23.492806958849112	26.610906657745065	25.205754432920706	24.690531950485113
27	24.877885580461694	25.687520910003347	25.05854800936768	24.37604550016728
28	24.146928944199118	26.24113475177305	25.91997858958919	23.691957714438647
29	24.215456674473067	25.453328872532623	24.84442957510873	25.486784877885583
30	24.215456674473067	25.44663767146203	25.70759451321512	24.630311140849784
31	24.663767146202744	26.1559049849448	24.99832719973235	24.182000669120107
32	24.877885580461694	26.12914018066243	25.105386416861826	23.88758782201405
33	23.974573435931752	25.687520910003347	25.3864168618267	24.95148879223821
34	24.871194379391103	24.75075276012044	25.593844095015054	24.7842087654734
35	24.93643784290111	26.120701190954097	24.722333734778537	24.22052723136625
36	23.037805286048847	26.75811308129809	25.406490465038473	24.797591167614584
37	24.74909674829386	25.77947276863375	25.25090325170614	24.22052723136625
38	24.280744011775727	25.47838886658638	24.829385788839822	25.411481332798076
39	24.797591167614584	25.48009367681499	24.764135162261624	24.9581799933088
40	25.264284758463802	26.528837147062763	24.842767295597483	23.364110798875952
41	24.294125518533388	25.759400508497254	25.886524822695034	24.05994915027432
42	25.33788304563094	26.348186805834338	24.9565101030376	23.357420045497122
43	23.84074941451991	26.262964202074272	25.33957845433255	24.55670792907327
44	23.732102234711625	26.160845711227083	25.150541951023687	24.9565101030376
45	24.280744011775727	26.87006556938311	24.722333734778537	24.126856684062624
46	23.859226548909408	26.749632008564166	24.126856684062624	25.264284758463802
47	24.882911815870468	26.267897765288375	25.07694366385655	23.77224675498461
48	25.551987153753515	26.970426870065566	23.832463535394087	23.645122440786835
49	24.294125518533388	26.903519336277267	24.36772380570052	24.434631339488828
50	24.670458347273335	25.915021746403475	24.616928738708598	24.797591167614584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	1.5
4	1.0
5	1.5
6	2.0
7	5.5
8	9.0
9	9.0
10	9.0
11	10.5
12	12.0
13	19.0
14	26.0
15	32.0
16	38.0
17	38.0
18	38.0
19	51.5
20	65.0
21	69.0
22	73.0
23	95.5
24	118.0
25	120.0
26	122.0
27	151.5
28	181.0
29	231.5
30	282.0
31	321.5
32	361.0
33	397.0
34	433.0
35	487.5
36	542.0
37	606.0
38	670.0
39	686.0
40	702.0
41	729.5
42	757.0
43	760.0
44	763.0
45	823.0
46	883.0
47	933.5
48	984.0
49	972.5
50	961.0
51	911.5
52	862.0
53	891.0
54	920.0
55	939.5
56	959.0
57	960.0
58	961.0
59	843.5
60	726.0
61	684.5
62	643.0
63	546.0
64	449.0
65	397.5
66	346.0
67	332.5
68	319.0
69	276.0
70	233.0
71	183.5
72	134.0
73	126.0
74	118.0
75	93.0
76	68.0
77	57.0
78	46.0
79	47.0
80	48.0
81	35.5
82	23.0
83	25.5
84	28.0
85	21.0
86	14.0
87	9.5
88	5.0
89	4.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006690753378830456
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.006690753378830456
16	0.006690753378830456
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.006690753378830456
26	0.006690753378830456
27	0.006690753378830456
28	0.0
29	0.006690753378830456
30	0.006690753378830456
31	0.006690753378830456
32	0.006690753378830456
33	0.006690753378830456
34	0.006690753378830456
35	0.0
36	0.006690753378830456
37	0.0
38	0.0
39	0.006690753378830456
40	0.0
41	0.0
42	0.0
43	0.006690753378830456
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.006690753378830456
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	14946.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.03693295865115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.49004407964736	83.18613675899907
2	3.343973248214014	5.887862973370801
3	0.8207934336525308	2.1678040947410677
4	0.44079647362821095	1.552254783888666
5	0.2051983584131327	0.9032517061421117
6	0.15199878400972794	0.8028904054596547
7	0.12159902720778233	0.749364378429011
8	0.06839945280437756	0.4817342432757929
9	0.037999696002431985	0.3010839020473705
>10	0.31919744642042863	3.967616753646461
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	25	0.1672688344707614	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	23	0.1538873277131005	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	23	0.1538873277131005	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	20	0.13381506757660913	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	20	0.13381506757660913	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	20	0.13381506757660913	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	19	0.12712431419777867	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	19	0.12712431419777867	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	19	0.12712431419777867	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	19	0.12712431419777867	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18	0.12043356081894822	No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	18	0.12043356081894822	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	17	0.11374280744011775	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	17	0.11374280744011775	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG	16	0.1070520540612873	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	15	0.10036130068245684	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	15	0.10036130068245684	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.006690753378830456	0.0
30	0.0	0.0	0.0	0.006690753378830456	0.0
31	0.0	0.0	0.0	0.013381506757660913	0.0
32	0.0	0.0	0.0	0.02007226013649137	0.0
33	0.0	0.0	0.0	0.02007226013649137	0.0
34	0.0	0.0	0.0	0.02007226013649137	0.0
35	0.0	0.0	0.0	0.02007226013649137	0.0
36	0.0	0.0	0.0	0.026763013515321826	0.0
37	0.0	0.0	0.0	0.04014452027298274	0.0
38	0.0	0.0	0.0	0.06690753378830457	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE