FastQCFastQC Report
Thu 2 Feb 2017
SRR4064108_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064108_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523584
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54390.2155268063199006No Hit
TATCAACGCAGAGTACTTTTTTTTT37360.14804341761558165No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG31610.12525836270954324No Hit
GGGTAGGCACACGCTGAGCCAGTCA28590.11329125561106743No Hit
GCGCAAGACGGACCAGAGCGAAAGC28150.11154770358347493No Hit
GAATAGGACCGCGGTTCTATTTTGT27650.10956639446121072No Hit
GTCTTGCGCCGGTCCAAGAATTTCA27060.10722844969693897No Hit
GATTAAGAGGGACGGCCGGGGGCAT26740.10596041185868986No Hit
GTATCTGATCGTCTTCGAACCTCCG25920.10271106489817657No Hit
CTATTGGAGCTGGAATTACCGCGGC25800.10223555070883315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA400.00527183314.25345117
ACTAGAC1001.0140866E-813.2945193
CGCATCG3700.012.32535313
AGTCGAG550.003067894412.09167910
CGGAATT1501.4551915E-1112.03600915
ATCGCCA3900.011.93855116
GTCCTAC13650.011.8980821
ACTCTAA4450.011.74232110
CGAACGA2300.011.56778816
TCGCGTA2150.011.4886479
GCGTAAC2150.011.48819211
GCGTTAT2150.011.4854581
TCCAACG3900.011.45194418
TCCTACA15300.011.4196762
GTATTAA2750.011.397111
CGCCAGT4100.011.35685918
AGGCCCG6450.011.34180710
CCAACGA3100.011.34168119
TTCGTTC2600.011.33011618
TTTCGTT2600.011.32966617
Produced by FastQC (version 0.11.5)